Multifunctional cell simulation cabin

A multi-functional, simulated cabin technology, applied in the direction of biochemical instruments, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as unstable oxygen concentration, simple design, and the influence of test gas cannot be ruled out. Easy access, precise control, and simple operation

Inactive Publication Date: 2016-02-10
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The design of this type of model is simple, the oxygen concentration in the cabin is unstable, the real-time detection of oxygen concentration cannot be realized, and the influence of the test gas itself on the test cannot be ruled out.

Method used

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Experimental program
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Embodiment Construction

[0015] 1. Intermittent hypoxia model

[0016] The original state is normal pressure and normal oxygen. Start the test program. After the high pressure nitrogen in the nitrogen cylinder (19) is collected by the nitrogen collection device (20) and adjusted for preliminary pressure, it is input to the nitrogen pressure reducer (21), and the nitrogen pressure is once again Adjust to 0.3KPa, pass through the nitrogen heat exchanger (22), the program controlled nitrogen solenoid valve (23) to open, the nitrogen flows through the nitrogen flow regulating valve (24), the nitrogen sterilization filter device (25) into the gas humidification device (12) ), the wet gas enters the test chamber body (27) through the gas silencer (9), and the mixing fan (30) is turned on at the same time to quickly and fully mix the gas filled into the test chamber. When the oxygen concentration in the chamber drops to the required level When the concentration is high, the nitrogen solenoid valve is closed, an...

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Abstract

The invention provides a multifunctional cell simulation cabin. The multifunctional cell simulation cabin is characterized by comprising an experimental cabin body, a PLC (programmable logic controller) and upper computer system, a nitrogen infusion device, an oxygen infusion device, a carbon dioxide infusion device, a concentration and humidity detecting device, an atomization device, a filtering device, a one-way valve and a mixing fan; the nitrogen infusion device, the oxygen infusion device and the carbon dioxide infusion device are connected to the inside of the experimental cabin body; according to preset requirements of environmental models, a certain proportion of gas can be inputted into the experimental cabin body through the nitrogen infusion device, the oxygen infusion device and the carbon dioxide infusion device, and various numerical values of the gas in the experimental cabin body are monitored in real time through the concentration and humidity detecting device, and are controlled through preset programs of an upper computer, so that starting and closing of the nitrogen infusion device, the oxygen infusion device and the carbon dioxide infusion device are controlled.

Description

Technical field [0001] The invention relates to a multifunctional cell simulation cabin, which is used for simulating a cell model of intermittent hypoxia, continuous hyperoxia, and special gas exposure. Background technique [0002] Obstructive sleep apnea hypopnea syndrome (Obstructivesleepapneahypopneasyndrom, OSAHS) is a common sleep disorder, mainly due to hypoventilation or sleep disturbance caused by repeated upper airway collapse or obstruction during sleep. The characteristic hypoxia of OSAHS is intermittent hypoxia, but its specific pathophysiological mechanism has not been clarified. In vitro cell experiments are widely used in the research of human diseases due to their single influencing factors, large maneuverability, and small differences between individuals. However, because oxygen and nitrogen are inert gases, it is difficult to effectively diffuse into the cell culture medium, and it is difficult to establish an effective intermittent hypoxic cell model. There...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/04C12Q3/00
Inventor 蔡晓红王华锐李秀翠王红霞林函曹红超梅红芳曹淑彦
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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