Method for bletilla striata seedling production based on liquid medium

The technology of a liquid culture medium and a production method is applied in the field of Bletilla striata seedling production based on the liquid culture medium, and can solve the problems of long time, large proliferation and usage, increased cost, etc., and achieves the effects of easy operation, low cost, and consistent emergence.

Active Publication Date: 2016-02-17
成都极谷基因科技有限公司
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with common hormones such as BA, TDZ is slightly more expensive. It is used in a large amount for proliferation and takes a long time, which increases the cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for bletilla striata seedling production based on liquid medium
  • Method for bletilla striata seedling production based on liquid medium
  • Method for bletilla striata seedling production based on liquid medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 immature bletilla striata culture

[0031] 1. Seed surface sterilization and germination

[0032] After 15 weeks of flowering and pollination, uncracked Bletilla striata capsules (including seeds) were surface-sterilized with 75% ethanol for 30 seconds on an ultra-clean workbench, and washed 1-2 times with sterile water. Carefully peel off the fruit with a scalpel and tweezers, carefully shake off the seeds into a sterile tissue culture bottle, add an appropriate amount of 5% sodium hypochlorite solution (1-2 drops of Tween 20) for disinfection for 20 minutes. Filter the seeds with a sterilized 50-mesh cell sieve; rinse and filter with sterile water, repeat 4-6 times, and wash away the residual disinfectant on the surface. Wash the filtered seeds on the cell sieve into the Erlenmeyer flask with MS+1.0mg / LTDZ+30g / L white granulated sugar, pH5.8 liquid seed germination medium. 25°C, 100rpm, dark culture for 15-20 days.

[0033] About 3 days later, the seed...

Embodiment 2

[0042] Example 2 Cultivation of nearly mature bletilla striata

[0043] 1. Seed surface sterilization and germination:

[0044] More than 20 weeks after flowering, the nearly mature Bletilla striata capsules (uncracked, containing seeds) are wrapped in gauze and washed with running water for 30 minutes; the surface is disinfected with 75% ethanol for 30 seconds on an ultra-clean workbench, and washed 1-2 times with sterile water . Carefully peel off the fruit with a scalpel and tweezers, carefully put the seeds into a sterile tissue culture bottle, add an appropriate amount of 5% sodium hypochlorite solution (add 2-3 drops of Tween) to disinfect for 20 minutes. Filter the seeds with a sterilized cell sieve (50 mesh); rinse and filter with sterile water, repeat 4-6 times, and wash away the residual disinfectant on the surface. Wash the filtered seeds on the cell sieve into the Erlenmeyer flask with MS+2.0mg / LTDZ+30g / L white granulated sugar, pH5.8 liquid seed germination liqu...

Embodiment 3

[0051] Embodiment 3 mature bletilla striata culture

[0052] 1. Seed surface sterilization and germination:

[0053] Mature Bletilla striata capsules (uncracked, containing seeds) were wrapped in gauze and rinsed with running water for 30 minutes; the surface was disinfected with 75% ethanol for 30 seconds on an ultra-clean workbench, and rinsed with sterile water 1-2 times. Carefully peel off the fruit with a scalpel and tweezers, carefully put the seeds into a sterile tissue culture bottle, add an appropriate amount of 5% sodium hypochlorite solution (add 2-3 drops of Tween) to disinfect for 20 minutes. Filter the seeds with a sterilized cell sieve (50 mesh); rinse and filter with sterile water, repeat 4-6 times, and wash away the residual disinfectant on the surface. Wash the filtered seeds on the cell sieve into the Erlenmeyer flask with MS+0.5mg / LTDZ+30g / L white granulated sugar, pH5.8 liquid seed germination liquid medium. 25° C., 100 rpm, and culture in the dark for 1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of traditional Chinese medicine materials and particularly relates to a method for bletilla striata seedling production based on a liquid medium. The to-be-solved technical problem is how to provide a novel method for quickly acquiring bletilla striata seedlings. The method comprises a step a of sterilization, a step b of germination, a step c of growth into buds, a step d of rooting culture and a step e of seedling hardening of adult seedlings and transplantation. The method is simple and convenient to operate and low in cost, and each bottle of liquid medium can be used for germinating several tens of thousands of seed. The seeds are germinated tidily, the seedlings emerge consistently, and the pollution rate is low. According to the method, the efficient quick production of the bletilla striata tissue culture seedlings is achieved finally, operating procedures are effectively simplified, the production cost is reduced, and large-scale production is facilitated.

Description

technical field [0001] The invention belongs to the technical field of planting Chinese medicinal materials, in particular to a method for producing bletilla striata seedlings based on a liquid medium. Background technique [0002] Bletilla striata is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae, and it is a national key protected wild plant. The flowers of Bletilla striata are large and colorful, and have high ornamental value. At the same time, Bletilla striata has massive pseudobulbs with thick flesh, which is one of the important Chinese medicinal materials. [0003] In the natural wild state, mature Bletilla striata seeds are powdery, very small, and mostly spindle-shaped under the microscope, about 1.2mm long and 0.2mm wide. The structure of its seeds is very simple, consisting of a transparent seed coat and a seed embryo. The seed coat is composed of a single layer of cells, the organelles and protoplasm have disappeared, and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 邓科君郑雪莲张勇
Owner 成都极谷基因科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap