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Method for rapidly identifying species of salted rhopilema esculentum kishinouye

A pickling and fast technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effect of high accuracy, strong specificity and high sensitivity

Active Publication Date: 2016-02-17
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far no one has applied this method to the identification of pickled jellyfish species

Method used

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  • Method for rapidly identifying species of salted rhopilema esculentum kishinouye
  • Method for rapidly identifying species of salted rhopilema esculentum kishinouye
  • Method for rapidly identifying species of salted rhopilema esculentum kishinouye

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Experimental program
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Effect test

Embodiment 1

[0053] 1. Desalinize the pickled cotton stings, change the water once every 12 hours, and change 4 times in total, use ethanol solution as the stock solution, store the dehydrated cotton stings in a solution with a final concentration of 70% ethanol (v / v), and store ;

[0054] 2. Take out 150 mg of the jellyfish stings stored in the ethanol solution, cut them into pieces, centrifuge at 10,000 rpm for 5 minutes, put them on a dust-free paper to absorb the water, and use the deep-processed food DNA extraction kit to extract jellyfish genomic DNA;

[0055] 3. Use 16SrRNA gene universal primers for PCR amplification, and store the product at -20°C;

[0056] 4. The PCR amplification product was digested with HindⅢ, HpaI, DraI and XhoI endonucleases, and the digested products were separated by 3% agarose gel electrophoresis and detected by imaging.

[0057] The result is as Figure 5 Shown, wherein, A represents HindⅢ, B represents HpaI, C represents XhoI, D represents DraI. It c...

Embodiment 2

[0060] 1. Purchase 4 kinds of commercially available pickled jellyfish from Shandong and Liaoning, numbered 1, 2, 3, 4, respectively, and carry out desalination treatment, change the water once every 12 hours, and change the water 4 times in total. Use ethanol solution as the stock solution, and dehydrate the jellyfish. The jellyfish is stored in a solution with a final concentration of 70% ethanol (v / v), for future use;

[0061] Among them, 1 represents the pickled jellyfish purchased from Yantai, Shandong, 2 represents the pickled jellyfish purchased from Yingkou, Liaoning, and 3 and 4 both represent the pickled jellyfish purchased from Dalian, Liaoning.

[0062] 2. Take out 150 mg of jellyfish stored in ethanol solution, cut it into pieces, centrifuge at 7500rpm for 10 minutes, put it on a dust-free paper to absorb the water, and use the deep-processed food DNA extraction kit to extract jellyfish genomic DNA;

[0063] 3. Use 16SrRNA gene universal primers for PCR amplificat...

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Abstract

The invention discloses a method for rapidly identifying species of salted rhopilema esculentum kishinouye. The method comprises steps as follows: preprocessing rhopilema esculentum kishinouye; extracting DNA (deoxyribonucleic acid); adopting rhopilema16S rRNA gene universal primers for PCR (polymerase chain reaction) amplification; performing RFLP (restriction fragment length polymorphism) analysis by the aid of restriction enzyme. The method has the characteristics of simplicity and high efficiency, the detection is convenient and is not time-consuming, the species of the commercially available salted rhopilema esculentum kishinouye can be rapidly determined, and the customer's benefit is guaranteed.

Description

technical field [0001] The invention relates to a method for identifying the species of pickled jellyfish in the market, and more specifically, relates to a rapid identification method for the species of pickled jellyfish. Background technique [0002] Cotton stinger (Rhopilema esculentum Kishinouye) is an important breed of jellyfish in northern my country. The jellyfish near the Liaodong Peninsula are mainly divided into cotton stinger and sand stinger (Stomolophus meleagris). Sand stings are low-grade products, while cotton stings are high-quality products of jellyfish. Usually, the market prices of the two kinds of jellyfish are: sand stings 3-5 yuan / catties, cotton stings 8-10 yuan / catties. The identification of cotton stingers and sand jellyfish is currently mainly focused on morphology, but after processing, it is not suitable for product identification in terms of morphology, so it is difficult for consumers to distinguish species. The phenomenon of filling is also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q2521/301C12Q2531/113
Inventor 吴海涛赵晨晨王凤林于翠平唐越孙娜阎佳楠石晓琳
Owner DALIAN POLYTECHNIC UNIVERSITY
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