Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of quickly detecting content of cell infective virions in circovirus

A virus particle and circovirus technology, applied in the field of biological genes, can solve the problems of long detection cycle, large human error, and inability to distinguish infectious virus particles, so as to shorten detection time, improve accuracy and repeatability Effect

Inactive Publication Date: 2016-02-17
SICHUAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECHN CENT +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has a long detection period, complicated operation steps, and large human error.
PCR / qPCR methods cannot distinguish between infectious and non-infectious virions
At present, there is no report to distinguish infectious and non-infectious circular virus particles by fluorescent quantitative PCR method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of quickly detecting content of cell infective virions in circovirus
  • Method of quickly detecting content of cell infective virions in circovirus
  • Method of quickly detecting content of cell infective virions in circovirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The present invention will be further described below in conjunction with the accompanying drawings and specific examples, but not as a limitation to the present invention.

[0025] 1. Test material

[0026] 1.1 Main test instruments

[0027] Bio-Rad C-1000 PCR instrument, Bio-Rad CFX96 fluorescence quantitative PCR instrument, ultra-low temperature refrigerator, ultraviolet spectrophotometer, high-speed centrifuge, biological safety cabinet, electrophoresis apparatus, chemiluminescent gel imaging system

[0028] 1.2 Test materials

[0029] Virus DNA / RNA magnetic bead method extraction kit was purchased from Chengdu Tuoyang Technology Co., Ltd.; fluorescence quantitative PCR detection reagent 2×SsoFastTMProbesSupermix was purchased from Bio-Rad, USA; DNA molecular weight standards and agarose were purchased from Bao Bioengineering (Dalian) Co., Ltd.; All other reagents were of domestic analytical grade. The circovirus vaccine strain was provided by Sichuan Huapai Bio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method of quickly and quantitatively detecting the content of cell infective virions in circovirus and belongs to the technical field of virus detection. The method comprises construction of a circovirus standard plasmid and establishment of a detection standard curve, a target cell is infected with a virus to be detected and a control virus under the same conditions, viral genomes infected with the virus to be detected and the control virus are extracted separately, and the relative contents of the virus to be detected and the control virus are obtained by fluorescent quantitative PCR (polymerase chain reaction) detection. A cell adsorption process is added before the step of fluorescent quantitative PCR detection, and the content of circovirus that is cell infective is quickly measured.

Description

technical field [0001] The invention relates to the field of biological genes, in particular to a method for detecting the content of infectious circular virus particles. Background technique [0002] Porcine circovirus (Porcine circovirus, PCV) is one of the smallest known mammalian viruses. It has two subtypes, PCV1 and PCV2. Among them, PCV1 is not pathogenic to pigs, and PCV2 is the cause of multisystemic wasting syndrome in weaned piglets. The main pathogen is one of the main diseases threatening the global pig industry. Porcine circovirus can replicate in porcine macrophages, porcine monocytes and other cells. In vitro, porcine circovirus can be propagated by PK-15 and IBRS-2 cells, but the virus does not produce cytopathic changes during the passage. Cannot grow on Vero cells, BHK-21 cells, ST cells, etc. At present, the detection methods of porcine circovirus mainly include virus isolation, indirect immunofluorescence test, immunoperoxidase monolayer test, PCR / flu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
Inventor 林华陈世界杨苗肖艳严玉宝胡娟赵姗薛昌华
Owner SICHUAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECHN CENT
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com