Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for biosynthesizing biocompatible gold nanoparticles using Staphylococcus aureus

A staphylococcus and biosynthesis technology, applied in the field of nano-gold, can solve the problem of long time consumption and achieve the effects of avoiding pollution, reducing energy consumption and high yield

Active Publication Date: 2018-02-16
SOUTHERN MEDICAL UNIVERSITY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages are: 1. The existing nano-gold synthesis method takes a long time, and this method can synthesize a large amount of nano-gold in a short time
However, it is still unknown whether it can synthesize gold nanoparticles.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for biosynthesizing biocompatible gold nanoparticles using Staphylococcus aureus
  • A method for biosynthesizing biocompatible gold nanoparticles using Staphylococcus aureus
  • A method for biosynthesizing biocompatible gold nanoparticles using Staphylococcus aureus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1) Configure 1mmol / L chloroauric acid (HAuCl 4 ) in aqueous solution.

[0066] 2) Add an appropriate amount of Staphylococcus aureus to 25ml of bacterial culture medium and culture overnight at 3000rpm to collect bacteria, and resuspend in 2ml of sterile water.

[0067] 3) Take 1ml of Staphylococcus aureus suspension (5×10 8 CFU / mL) with 2ml HAuCl 4 Stir magnetically, mix well, and react for 6 hours.

[0068] 4) The reaction solution in the previous step was centrifuged at 4000rpm to remove precipitated impurities, and the supernatant was reacted at 20°C for 48h, and centrifuged at 12000rpm for 30min to collect the precipitate, that is, gold nanoparticles.

[0069] Precipitated gold nanoparticles obtained in the present embodiment such as figure 1 As shown in the bottom part of the centrifuge tube in Figure A, it turns pink after being resuspended in water. figure 1 As shown in Figure B (inverting the centrifuge tube can be more convenient and more accurate to obse...

Embodiment 2

[0074] 1) Configure 1mmol / L chloroauric acid (HAuCl 4 ).

[0075] 2) Add appropriate amount of Staphylococcus aureus to 25ml bacterial culture medium and culture overnight at 3000rpm to collect bacteria, and resuspend in 2ml sterile water.

[0076] 3) Take 1ml of Staphylococcus aureus suspension (5×10 8 CFU / mL) with 2ml HAuCl 4 Stir magnetically, mix well, and react for 6 hours.

[0077] 4) Collect the obtained product, centrifuge at 4000rpm to remove precipitated impurities, take the supernatant and react it in a water bath at 80°C for 0.5h, and centrifuge at 12000rpm for 30min to collect the precipitate as gold nanoparticles.

[0078] After resuspending the precipitated gold nanoparticles obtained in this embodiment with water, it is pink (such as image 3 The inset in ), its UV-visible absorption curve is shown as image 3 As shown, there is only a single absorption peak at 524nm, indicating that the nano-gold particle size distribution range is 5-20nm, and the purity ...

Embodiment 3

[0081] 1) Configure 10mmol / L chloroauric acid (HAuCl 4 ).

[0082] 2) Add appropriate amount of Staphylococcus aureus to 25ml bacterial culture medium and culture overnight at 3000rpm to collect bacteria, and resuspend in 2ml sterile water.

[0083] 3) Take 1ml of Staphylococcus aureus suspension (5×10 8 CFU / mL) with 2ml HAuCl 4 Mix well and react for 6 hours.

[0084] 4) Collect the obtained product, centrifuge at 4000rpm to remove precipitated impurities, take the supernatant and react in a water bath at 80°C for 0.5h, and centrifuge at 12000rpm for 30min to collect the precipitate, that is, gold nanoparticles.

[0085] After resuspending the precipitated gold nanoparticles obtained in this embodiment with water, it is blue-purple (such as Figure 4 The inset in ), its UV-visible absorption curve is shown as Figure 4 As shown, there is only a single absorption peak at 540nm, indicating that the nano-gold particle size distribution range is 30-80nm, and the purity is hi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for biosynthesis of nanogold with biocompatibility through staphylococcus aureus. The method comprises the steps that staphylococcus aureus liquid and chloroauric acid are evenly mixed, and mixed liquor is made to react for 4-6 h; reaction liquor is centrifuged, supernatant is collected, and the reaction continues for 0.5-48 h; and reaction liquor is centrifuged again, and the nanogold can be obtained after sediment is collected. The nanogold obtained through biosynthesis has the biocompatibility and has no toxicity on cells; the particle size of the obtained nanogold is uniform and controllable, and the nanogold of the needed particle size can be synthesized according to requirements. The obtained nanogold can be kept stable without agglomeration for a long time, and the nanogold can be kept stable without the need of interfacial agents. According to the method, as biosynthesis is carried out at the room temperature and under the room pressure, pollution caused by organic solvents can be avoided, and energy consumption can be reduced; the staphylococcus aureus is easy and convenient to obtain, economical and renewable.

Description

technical field [0001] The invention relates to a method for biosynthesizing nano gold with biocompatibility by staphylococcus aureus. Background technique [0002] Nanogold: also known as "colloidal gold", because it is easy to gather into blocks in the air, so nanogold is generally prepared in a solution system. Nanogold in solution with a size below 100 nanometers usually makes the solution red, while a size larger than 100 nanometers makes the solution blue or purple. Nanogold has special optical properties, electrical properties, molecular recognition properties and good biocapacity, making nanogold the most widely studied nanomaterial, and has been used in various fields, such as electron microscopy, electronics , material science, biological detection, optical detection, drug delivery, catalytic reaction, disease treatment, electronic engineering and template crystallization. [0003] At present, the synthesis methods of nano gold include physical synthesis, chemica...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B82Y40/00
CPCB22F9/24B82Y40/00
Inventor 邱小忠华文熙王乐禹
Owner SOUTHERN MEDICAL UNIVERSITY