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Purifying method of corn starch 3-grade particles

A corn starch and purification method technology, applied in the field of purification of corn starch granules of different sizes, can solve the problems of insufficient starch, consume a lot of time, and the separation liquid is expensive, so as to overcome the high price, low purification cost and good purification effect. Effect

Active Publication Date: 2016-02-24
JIANGSU FOCUS AGRICULTURE SCIENCE AND TECHNOLOGY CO. LTD.
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some defects in the above several methods: 1. The separation effect of the step-by-step natural sedimentation method is better, but a) the amount of separated starch granules is very small each time, because the concentration of the starch granules should be controlled at 2mg / mL to ensure the sedimentation Following Stokes' law, the amount of starch obtained each time is insufficient; b) the separation of each level takes a lot of time to ensure the purity of each level; ③ The centrifugal sedimentation method can separate a large number of starch granules, but a) the purity of each part of the starch granules separated from sucrose-water solution and maltose-water solution is not high; b) the separation effect of Precoll cell separation liquid is very good, and the purity is very high. However, the separation liquid is expensive and not suitable for separating large amounts of starch granules

Method used

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  • Purifying method of corn starch 3-grade particles
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  • Purifying method of corn starch 3-grade particles

Examples

Experimental program
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Effect test

Embodiment 1

[0067] Embodiment 1: the purification of common corn starch granule: (the flow process of whole purification sees figure 1 )

[0068] (1) Prepare starch suspension:

[0069] ① Prepare 85% glycerin-water solution: 212.5mL pure glycerin + 37.5mL distilled water, mix well;

[0070] ②Weigh 1.0g of ordinary corn starch (purchased from Penford Company) into six 50mL plastic round-bottomed centrifuge tubes (group A centrifuge tubes), add 40mL85% glycerol-water solution to each of the six centrifuge tubes, and make the concentration 2.5% starch suspension;

[0071] (2) Stir evenly: use a glass rod to gently stir the starch suspension in the centrifuge tube for 5 minutes, so that the starch particles are evenly dispersed in the glycerol-water solution;

[0072] (3) 85% glycerol-water solution centrifugation:

[0073] ① Centrifugation: 6 centrifuge tubes were centrifuged immediately at 100g for 5 minutes, and the supernatant was carefully drawn into a beaker with a pipette for purif...

Embodiment 2

[0100] Embodiment 2: the purification of high amylose corn starch granules: (see the flow process of whole purification figure 1 )

[0101] (1) Prepare starch suspension:

[0102] ① Prepare 85% glycerin-water solution: 212.5mL pure glycerin + 37.5mL distilled water, mix well;

[0103] ② Weigh 1.0 g of high-amylose corn starch (purchased from Penford, Australia, number: Gelose50) into six 50 mL plastic round-bottomed centrifuge tubes (group A centrifuge tubes), and add 40 mL of 85% glycerol to each of the six centrifuge tubes- Aqueous solution, made into a starch suspension with a concentration of 2.5%;

[0104] (2) Stir evenly: use a glass rod to gently stir the starch suspension in the centrifuge tube for 5 minutes, so that the starch particles are evenly dispersed in the glycerol-water solution;

[0105] (3) 85% glycerol-water solution centrifugation:

[0106] ① Centrifugation: 6 centrifuge tubes were centrifuged immediately at 100g for 5 minutes, and the supernatant was...

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Abstract

The invention discloses a purifying method of corn starch 3-grade particles. According to the purifying method, corn starch particles are divided into large particles (with a particle size larger than 15<mu>m), middle particles (with a particle size ranging from 10 to 15<mu>m), and small particles (with a particle size smaller than 10<mu>m). The purifying method comprises following steps: starch suspension preparation; uniform stirring; adding of 85% glycerinum-water solution and centrifugation; collection of large starch particles; adding of 55% glycerinum-water solution and centrifugation; and collection of middle and small starch particles. The purifying method is excellent in purifying effect; operation is simple and convenient; time is saved; cost is low; the purifying method is capable of providing technical support for research on physical and chemical properties of corn starch particles with different particle size, and is helpful for processing and application of corn particles.

Description

technical field [0001] The invention relates to the field of purification of starch granules of different sizes, in particular to a purification method of corn starch granules of different sizes. Background technique [0002] Starch granules are semi-crystalline granular structures composed of amylose and amylopectin, which are synthesized in amyloplasts and are important energy storage substances in plants. The size of starch granules is an important parameter affecting its functional properties. Starch granules of different sizes are endowed with different uses in both food and non-food industries. The particle size can be expressed by the average particle size of starch particles, the average value of the short axis and long axis, the maximum diameter, volume, etc. According to different plant sources, the size of starch particles ranges from 0.5 μm to 100 μm, such as canna starch The diameter of the grain is up to 100 μm; the potato starch grain is 15-75 μm; the corn st...

Claims

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Application Information

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IPC IPC(8): C08B30/04
Inventor 裴柏平陈志华陈玲丁红梅韦平
Owner JIANGSU FOCUS AGRICULTURE SCIENCE AND TECHNOLOGY CO. LTD.
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