Method for cultivating wild truffles
A technology of wild truffles, truffles, applied in the directions of microorganism-based methods, botanical equipment and methods, biochemical equipment and methods, etc., to achieve the effect of high survival rate
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[0029]A. Pick fresh and large wild truffles, scrape off the skin with a knife to clean the soil, then clean the skin of the truffle with 75% alcohol by volume, cut off the skin with a knife under sterile conditions, and clean the surface with alcohol , cut into small pieces with a knife, the length is 1cm, the thickness is 0.2cm, and the width is 0.3cm;
[0030] B. Inoculate the massive truffles obtained in step A into the following medium: 1200 parts of cornmeal, 1200 parts of soybean flour, 1440 parts of pine oil, 1200 parts of glucose powder, 5.2 parts of vitamin B, 46 parts of yeast, and sodium selenite 1.2 parts ﹑ 1.2 parts calcium carbonate ﹑ 4.8 parts magnesium sulfate ﹑ 4.8 parts potassium dihydrogen phosphate ﹑ Incline culture at 26°C for 25 days to obtain truffle strains, its preservation number: CGMCCNO, No. 11601;
[0031] C﹑Inoculate the wild truffle strain with the preservation number CGMCCNO11601 into the culture medium of the nutrition bag, cut the surviving pi...
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