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Method for quickly multiplying detoxicated garlic seedlings as seed

A technology for seedlings and garlic, applied in the field of virus-free and rapid propagation of garlic seedlings, and the field of propagation of garlic-free seedlings, can solve the problems of non-compliance with virus-free and rapid propagation, variation, etc., and achieve simple operation, high survival rate, The effect of low labor intensity

Inactive Publication Date: 2002-09-18
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on directly obtaining virus-free seedlings with garlic sprouts as explants. Using garlic sprouts to induce the formation of callus and then differentiate into seedlings will inevitably cause variation, which does not meet the requirements of virus-free rapid propagation to maintain species requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) Obtaining of virus-free test-tube seedlings: the tested variety is Taicang white garlic (Allium sativum L.). Take uncooked garlic sprouts as explants, disinfect the surface with 70% ethanol for 1 minute, then disinfect with 0.1% mercuric chloride for 15 minutes, rinse with sterile water 4 times, peel off the outer bract leaves, and cut the sprout tip into four Received in the same bottle, with B 5 For basic medium, add 1.0mg / L BA and 0.1mg / L NAA. After 60 days of cultivation, 50 seedlings can be obtained from each shoot.

[0016] 2) Induction and expansion of test tube bulbs: select test tube seedlings with uniform growth, cut the base of the seedlings and inoculate them in B 5 In the basic medium, add 120g / L sucrose and 0.5mg / L ethephon. Adjust the pH to 6.4 before sterilization. The temperature of the cultivation room is 25°C±2°C, the light is 14 hours a day, and the light intensity is 2000Lux. After 85 days, the bulb formation rate is 85%, and the fresh weight...

Embodiment 2

[0018] 1) Acquisition of virus-free test-tube seedlings: the variety of garlic tested was Xuzhou cabbage (Allium sativum L.). Take uncooked garlic sprouts as explants, disinfect the surface with 70% ethanol for 1 minute, then sterilize with 0.1% mercuric chloride for 15 minutes, rinse with sterile water for 4 times, peel off the outer bract leaves, cut the tips of the sprouts and inoculate them. In the same bottle, use MS as the basic medium, add 0.5mg / LBA and 0.06mg / L NAA. After 50 days of cultivation, 65 seedlings can be obtained from each shoot. Choose the test-tube seedlings that grow neatly and uniformly, and cut the base of the seedlings as the processing material.

[0019] 2) Bulb induction and expansion in test tubes: select test tube seedlings with uniform growth, cut out the base of the seedlings and inoculate them in MS basic medium, add 4.0 mg / L BA, 90 g / L sugar, and 10 mg / L ethephon. Adjust the pH to 6.0 before sterilization. The temperature of the cultivation ...

Embodiment 3

[0021] The variety of garlic used in the test was Xuzhou white garlic, and immature garlic sprouts were taken as explants. According to the above method, after adjusting the composition of the medium, the results were as follows:

[0022] 1) test tube seedling medium: B 5 +2.0mg / L BA+0.2mg / L NAA; medium for inducing bulb formation and promoting bulb expansion: B 5 +3.0mg / L BA+0.1mg / L NAA+white sugar 100g / L+ethephon 20mg / L, after 80 days, the bulb formation rate was 82%, the fresh weight of the bulb was 300mg, and the survival rate of the bulb transplanted in the field was 95.6%.

[0023] 2) Test-tube seedling culture medium: B 5 +3.0mg / L BA+0.2mg / L NAA; medium for inducing bulb formation and promoting bulb expansion: B 5 +2.5mg / L BA+0.1mg / L NAA+sucrose 100g / L+ethephon 0.5mg / L, after 70 days, the bulb formation rate was 83%, the bulb fresh weight was 320mg, and the survival rate of bulb transplanted in field was 95.8%.

[0024] 3) Test-tube seedling culture medium: B 5 +2.5...

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Abstract

A method for quickly multiplying the detoxicated garlic seedlings as seed features use of unripened garlic bolts as explant, selecting culture medium and culture time, changing the kind of saccharide, its concentration and hormone ratio, and use of jasmonic acid and salicylic acid as new plant hormones to obtain a lot of detoxicated test-tube seedlings. Its reproductive base number can reach 50-76. The inductivity of test-tube bulb is more than 80%. The weight of fresh bulb is more than 300 mg. Obtained bulb can directly be transplanted to field. Its advantages are high speed and high detoxicating effect.

Description

technical field [0001] The invention relates to a method for breeding virus-free garlic seedlings, which belongs to the technical field of vegetable seedling production and is specially used for the virus-free and rapid propagation of garlic seedlings. Background technique [0002] Garlic (Allium sativum L.) is a worldwide vegetable that is used both as medicine and as a vegetable, and is also an important foreign exchange earning vegetable for export in my country. Because of long-term asexual reproduction with garlic cloves, the virus accumulates, the species degrades, and the garlic head (bulb) becomes smaller, which seriously damages its commodity quality and weakens its international and domestic market competitiveness. Efforts to improve garlic (bulb) quality is a top priority. The currently commonly used detoxification method is combined with heat treatment for shoot apex tissue culture, but the reproduction coefficient is low, usually only 2 to 3 seedlings can be ob...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 熊正琴李式军刘高琼黄保健周素平
Owner NANJING AGRICULTURAL UNIVERSITY
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