Bacillus stratosphericus strain and application thereof

A bacillus and stratosphere technology, applied in the direction of application, bacteria, chemicals for biological control, etc., can solve the problems of nutrient element decline, lack of research on dry farming tobacco fertilization technology, and nutritional imbalance

Active Publication Date: 2016-02-24
CHINA TOBACCO HUNAN INDAL CORP
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The reason is that there is a lack of in-depth research on the correlation technology of fertilizer use efficiency, especially the control of fertilizer loss in southern tobacco areas and the nutrient absorption obstacles in northern tobacco areas have not been fundamentally solved; (2) the soil fertility of tobacco fields is different. There is a downward trend, due to the lack of good soil fertility measures, coupled with poor crop rotation conditions, continuous cropping in tobacco fields all year rou

Method used

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  • Bacillus stratosphericus strain and application thereof
  • Bacillus stratosphericus strain and application thereof
  • Bacillus stratosphericus strain and application thereof

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with embodiment.

[0026] Stratospheric Bacillus (Bacillus stratosphericus) PR1 of the present invention, preservation unit: China Microbiological Culture Collection Management Committee General Microbiology Center (CGMCC), address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, preservation date: August 2014 On March 28, the deposit number: CGMCCNo.9612.

[0027] (1) Screening of CGMCCNo.9612

[0028]Enrichment process in beef extract peptone liquid medium: Take tobacco rhizosphere soil collected from Hunan Sangzhi Guandiping and Liuyang Tobacco Institute, a total of 10 samples, numbered G1, G2, G3, G4, G5, L1 , L2, L3, L4, L5, each soil sample was weighed 10g, added to 100mL beef extract peptone liquid medium, 191r / min, 37°C shaker shaking culture for 24h.

[0029] The process of degrading protein with skimmed milk powder as the substrate: after taking the enriched culture solution a...

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Abstract

The invention discloses a Bacillus stratosphericus strain and application thereof. The strain is classified and named as Bacillus stratosphericus PR1, collected at the China General Microbiological Culture Collection Center, in Number 3 of Number 1 yard on the Beichen west road in the Chaoyang District of Beijing, on 28th, August, 2014 and numbered as CGMCC No.9612. The strain has good degrading effect on protein, and enzyme activity, of protease generated by the strain is 7.493 IU, measured by a Folin-phenol method; the strain has good phosphorus dissolving effect measured by using a phosphate dissolving ring method and a molybdenum blue spectrophotometry method; the strain has good green-keeping effect by using a green-keeping method, and cytokinin can be generated. It shows that the PR1 strain has potential application value in promoting tobacco growth.

Description

technical field [0001] The invention belongs to the technical field of plant rhizosphere growth-promoting bacteria and the application thereof, and specifically relates to a stratosphere bacillus capable of degrading protein, dissolving phosphorus, and producing cytokinin to promote the growth of tobacco and the application of the growth-promoting characteristics thereof. Background technique [0002] Tobacco is an annual herbaceous plant belonging to the genus Nicotiana in the family Solanaceae. Likes temperature, likes light, tolerates drought, is afraid of waterlogging, tolerates barrenness, and needs more potassium. Tobacco leaves, stems, calyxes and fruits all have multicellular hairs, and the glandular hair cells contain chlorophyll, which can synthesize exudates containing gums and resins, which have a certain relationship with the aroma of tobacco. Tobacco petioles are inconspicuous or winged, with terminal panicles, tubular calyx, funnel-shaped corolla, and pink at...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/07
Inventor 周曙光易建华周东波王东蒲文宣汪耀富孙在军彭宇简永兴杜秉海丁延芹姚良同
Owner CHINA TOBACCO HUNAN INDAL CORP
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