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A method for increasing epa and dha content in glyceride type fish oil

A technology for glyceride-type and ethyl-ester-type fish oil, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of large amount of commercial lipase, limited industrial production, and high production cost, and achieves strong production efficiency. Transesterification activity, low production cost, and low environmental pollution

Active Publication Date: 2019-05-31
ZHEJIANG UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all can improve the content of EPA and DHA in the glyceride to a certain extent, but the consumption of the commercialized lipase of these methods is big, and production cost is high, thereby has limited its suitability for industrialized production

Method used

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  • A method for increasing epa and dha content in glyceride type fish oil
  • A method for increasing epa and dha content in glyceride type fish oil
  • A method for increasing epa and dha content in glyceride type fish oil

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Slant cultivation: Aspergillus oryzae WZ007 (preservation number CCTCC No. M 206105) was inoculated into the slant medium and cultured at 30° C. for 3 days to serve as slant activation seeds. Final concentration composition of slant medium quality: NaNO 3 0.2%, K 2 HPO 4 0.1%, KCl 0.5%, FeSO 4 0.001%, MgSO 4 0.05%, 3% sucrose, 1.5% agar, deionized water as solvent, natural pH, sterilized at 121°C for 20 minutes.

[0021] Fermentation culture: inoculate slant seeds into 1L liquid fermentation medium, culture at 30°C and 200r / min for 48 hours, filter the culture solution, and obtain 80g wet weight of mycelia. The final concentration of the liquid fermentation medium consists of: glucose 20g / L, peptone 20g / L, sucrose 10g / L, urea 3g / L, NaCl 5g / L, K 2 HPO 4 2g / L, MgSO 4 1g / L, (NH 4 ) 2 SO 45g / L, the solvent is deionized water, the pH is natural, the liquid volume is 80ml in a 500ml Erlenmeyer bottle, and it is sterilized at 121°C for 20 minutes.

[0022] 80 g...

Embodiment 2

[0023] Embodiment 2: the preparation of ethyl ester type fish oil

[0024] Preparation of ethyl ester type fish oil (EPA and DHA total content 60-70%, EPA mass content 35-40%, DHA mass content 25-30%):

[0025] Sardine fish oil (21% EPA mass content, 9% DHA mass content) was enriched by molecular distillation and urea embedding method. Using 21% EPA mass content and 9% DHA mass content sardine fish oil as raw material, carry out molecular distillation in POPE 2inch WFS molecular distillation apparatus (POPE Science Company, USA). Add urea for urea embedding, and prepare ethyl ester type fish oil with 38% EPA mass content and 28% DHA mass content, wherein molecular distillation conditions: distillation temperature 90°C, preheating temperature 40°C, internal cooling temperature 15°C, scraping film The concentration of ethylated fish oil was carried out under the conditions of 360r / min rotating speed, 2mL / min feeding rate and 0.1Pa vacuum degree. Urea embedding conditions: mass...

Embodiment 3

[0027] 1g of Aspergillus oryzae WZ007 freeze-dried thalline prepared by the method of Example 1, anchovy fish oil (11% of EPA mass content, 14% of DHA mass content) and ethyl ester type fish oil (prepared by the method of Example 2, EPA mass content of 38%) , DHA mass content 28%) each 5ml (glyceride and ethyl ester molar ratio 1:3), under 70 ℃, 200rpm condition, after water bath reaction 20h, monitor the total content of EPA and DHA in the ethyl ester type fish oil by gas chromatography Change, when the total content of EPA and DHA is stable, end the reaction, filter the reaction solution, collect the dry cells, distill the filtrate under reduced pressure until no liquid flows out (50Pa, 240°C) to remove EPA\DHA ethyl ester, and obtain the concentrated The product is a high-content EPA and DHA glyceride type fish oil, and the total content of EPA and DHA is 50.2% after testing, of which EPA content is 29.2%, and DHA content is 21.0%.

[0028] Detection method of EPA and DHA c...

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Abstract

The invention discloses a method for improving contents of EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) in glyceride type fish oil. According to the method, the glyceride fish oil, which is high in EPA\DHA content, is synthesized by taking a freeze-dried strain as a catalyst and taking glyceride type fish oil and ethyl ester fish oil as substrates, and by conducting an ester interchange reaction under the action of aspergillus oryzae lipase, wherein the freeze-dried strain is prepared by freeze-drying a wet strain which is obtained from the fermented culture of aspergillus oryzae WZ007 after suspending the wet strain with a phosphate buffer which is 7.0 in pH value. The lipase adopted by the intermediate product preparation method disclosed by the invention is high in catalysis efficiency and the catalyst is recyclable; and the method is simple in downstream separation, low in energy consumption, low in production cost and light in environment pollution, and the method is applicable to industrial production.

Description

(1) Technical field [0001] The present invention relates to a method for increasing the content of EPA and DHA in fish oil, in particular to a method of using glyceride type fish oil and ethyl ester type fish oil as reaction substrates, and carrying out ester exchange reaction under the action of Aspergillus oryzae lipase to increase glycerol Method for EPA and DHA content in ester-type fish oils. (2) Background technology [0002] cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and cis-4,7,10,13,16,19-docosahexaenoic acid (DHA) are two The important n-3 polyunsaturated fatty acids (PUFA) have important nutritional and health effects on the human body. The content of EPA and DHA in naturally occurring fish oil is low, and the glyceride form of EPA and DHA is easily digested and absorbed by the human body. Therefore, the preparation of fish oil products with high content of EPA and DHA glyceride has become a research hotspot in this field. Traditional physical and chemical met...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/69
CPCY02P20/584
Inventor 汪钊郑建永王升帆许新德章银军邵斌
Owner ZHEJIANG UNIV OF TECH
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