A selective medium for identification of Vibrio cholerae

A selective technology of Vibrio cholerae, applied in the field of microbial identification and detection, can solve the problems of low sensitivity, long detection process, affecting detection results, etc.

Inactive Publication Date: 2019-01-01
林青
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method is low cost and easy to implement, it may affect the detection results due to the growth of other alkaliphiles
On the other hand, the results of gene sequencing are the most reliable, so this method is usually used in the scientific research field. However, because this method requires complex operations such as DNA extraction, PCR amplification, and sequenci...

Method used

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  • A selective medium for identification of Vibrio cholerae
  • A selective medium for identification of Vibrio cholerae

Examples

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Effect test

Embodiment 1

[0018] A selective culture medium for identification of Vibrio cholerae, consisting of the following components by weight: 8 parts of peptone, 4 parts of silkworm chrysalis powder, 2 parts of poria cocos powder, 1 part of alfalfa powder, 1 part of walnut powder, and 1 part of black soybean hulls , 1 part of lactose, 1 part of acacia rice, 1 part of Chaga fungus, and 1 part of p-dimethylaminobenzaldehyde.

Embodiment 2

[0020] A selective culture medium for identification of Vibrio cholerae, consisting of the following components by weight: 10 parts of peptone, 6 parts of silkworm chrysalis powder, 3 parts of poria cocos powder, 2 parts of alfalfa powder, 2 parts of walnut powder, and 2 parts of black soybean hulls , 2 parts of lactose, 2 parts of acacia rice, 2 parts of Chaga fungus, and 2 parts of p-dimethylaminobenzaldehyde.

Embodiment 3

[0022] A selective culture medium for identification of Vibrio cholerae, consisting of the following components by weight: 9 parts of peptone, 5 parts of silkworm chrysalis powder, 2.3 parts of poria cocos powder, 1.8 parts of alfalfa powder, 1.5 parts of walnut powder, and 1.6 parts of black soybean hulls , 1.7 parts of lactose, 1.8 parts of acacia rice, 1.2 parts of Chaga fungus, and 1.8 parts of p-dimethylaminobenzaldehyde.

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Abstract

The invention provides a selective culture medium for identifying Vibrio cholerae. In the technical scheme, on the basis of nutrition metabolism characteristics of Vibrio cholerae, the inventor preferably selects nutrition facts suitable for growth of Vibrio cholerae, and experiments find that growth of most gram positive bacteria is obviously inhibited under the nutrition condition, so that range of microorganisms possibly exerting an influence on detection results is narrowed. On this basis, the characteristic that Vibrio cholerae can decompose tryptophan in peptone to generate indole is utilized, and a proper amount of paradimethylaminobenzaldehyde is added to serve as a selective indicator which can react with indole to generate red rosindol so as to serve as a positive indicator of Vibrio cholerae. Protruding technical effect is realized through creative technical improvement, and the selective culture medium is low in cost and easy to implement, thereby having a protruding popularization prospect.

Description

technical field [0001] The invention relates to the technical field of microorganism identification and detection, in particular to a selective culture medium for identification of Vibrio cholerae. Background technique [0002] As an ancient and widespread severe infectious disease, cholera has caused many pandemics in the world, mainly manifested as severe vomiting, diarrhea, dehydration, and high mortality rate. It belongs to the international quarantine infectious disease. Vibrio cholerae is the pathogen of human cholera. It belongs to Gram-negative bacteria. Its body is curved in the shape of an arc or a comma. There is a single flagella and pili at one end of the body. It has no capsule and spores. After artificial cultivation, it is easy to lose Curved and rod-shaped. Because cholera is highly contagious and has a high mortality rate, the detection technology of Vibrio cholerae is very important for public health safety. [0003] The strain has low nutritional requir...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
CPCY02A50/30
Inventor 林青
Owner 林青
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