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Eurotium cristatum strain and breeding method and application thereof

The technology of Coronidium coronoidi strain and Coronidium coronoidum strain is applied in the fields of microorganisms and food biology, which can solve the problems of unclear strain selection process, shortened fermentation period, unclearness, etc. The effect of stable flower quantity and controllable cycle

Active Publication Date: 2016-03-02
中国土产畜产进出口有限责任公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0006] Chinese patent document CN201310195681.7 (application number) uses CGMCCNo.5891 to ferment Fuzhuan brick tea, which is a breakthrough in tea fermentation technology, and realizes pure fermentation of a single strain, but the breeding process of this strain is not clear. It can grow rapidly under unfavorable conditions such as high temperature, whether it can shorten the fermentation cycle to the greatest extent, whether the genetic stability can achieve long-term stability to meet the needs of the factory, and whether the strain is suitable for fermentation of other tea varieties is not yet clear
And the application of above-mentioned strains is all limited to a certain tea variety

Method used

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  • Eurotium cristatum strain and breeding method and application thereof

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preparation example Construction

[0059] The preparation process of the golden flower dark tea is improved from the traditional Fuzhuan brick tea, and also includes initial preparation, screening, steaming, and fermentation, and the flowering process is preferably after fermentation;

[0060] The preparation process of Jinhua Liubao tea also includes initial preparation, screening, fermentation, drying, autoclaving, and aging in cellars, and the flowering process is preferably after aging in cellars;

[0061] The preparation process of various teas such as Jinhua Puer tea, Jinhua Dahongpao, Jinhua black tea, Jinhua white tea or Jinhua green tea also includes the conventional initial preparation and refining steps of various tea leaves, and the flowering process is preferably refined. after.

[0062] The raw materials used in Examples 1-15 of the present invention are obtained through initial preparation and refining according to the traditional procedures of various tea leaves. Wherein the flowering experimen...

Embodiment 2

[0064] Embodiment 2 and embodiment 3 increase the inoculation process on the basis of embodiment 1. Embodiment 1 is the traditional natural flowering of Anhua black tea Fu brick tea. The flowering process used in embodiment 2 and embodiment 3 is different in removing bacteria. In addition, other process conditions are completely the same, wherein the strain in Example 2 is the Chrysanthemum chrysogenum strain that has not been bred in step S5, and the strain in Example 3 is the Mycocystis coronis strain with the preservation number CGMCC NO.11304 of the present invention. Wherein the blooming process includes steaming and humidification, inoculation and flowering and drying, the autoclaving and humidification is to autoclave for 0.5min under the condition of pressure 0.2MPa, and humidify to 20%-35% of the tea moisture, and inoculation and flowering are inoculated. Into 10×10 6 pcs / kg tea solid seeds; the inoculation and flowering are carried out after the inoculation of the st...

Embodiment 5

[0065] Embodiment 5 and embodiment 6 have increased the inoculation process on the basis of embodiment 4, and embodiment 4 has carried out steaming humidification, flowering and drying on the basis of traditional technology, and the flowering process has not been inoculated, and embodiment 5 and The flowering process used in Example 6 is identical except for the different strains, and the other process conditions are exactly the same, wherein the strain in Example 5 is the golden flower fungus that has not been bred in step S5, and the preservation number of the present invention in Example 6 is CGMCCNO. 11304 strains of Cystis coronaris. Wherein the blooming process includes steaming and humidification, inoculation and flowering, and drying. The steaming and humidification is to autoclave for 20 minutes under a pressure of 0.2 MPa, and to humidify to a tea moisture of 20%-35%. 10×10 6 pcs / kg tea solid seeds; the inoculation and flowering are carried out after the inoculation...

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Abstract

The invention belongs to the technical field of microorganisms and food biology, and particularly relates to a eurotium cristatum strain which has been preserved in the common microorganism center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the eurotium cristatum strain is CGMCC NO.11304. Strict breeding authentication shows that the eurotium cristatum strain has a series of characteristics suitable for tea leaf production and fermentation and can be used for producing various golden flower tea leaves such as golden flower dark tea, golden flower Liupu tea, golden flower Pu'er tea and golden flower clovershrub tea, and produced tea leaves are clear in characteristic and good in quality.

Description

technical field [0001] The invention belongs to the field of microbiology and food biotechnology, and in particular relates to a coronoid loose capsule strain, its breeding method and its use in the fermentation process of various teas such as Anhua black tea, Liubao tea, Pu'er tea, Dahongpao tea or black tea. Applications. Background technique [0002] Coronoidis is the earliest fungus isolated and identified from Anhua black tea Fuzhuan tea. It multiplies in large numbers during the late fermentation process of Anhua dark tea, and forms a large number of golden closed capsules in the tea, so it is also named "Golden Flower Fungus". Anhua Dark Tea Fuzhuan Tea also has a unique process of "flowering". . Coronarysis coronis secretes a large amount of secondary metabolites such as enzymes and polysaccharides, which promote the full transformation of substances in dark tea. Therefore, Fuzhuan tea has a special "fungal fragrance". The aroma, taste, and soup color of Fuzhuan te...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23F3/08A23F3/14C12R1/645
Inventor 郝彬秀陈然李颂田海霞马跃王春玲
Owner 中国土产畜产进出口有限责任公司
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