- A method for preparing protein peptides from Phnom Penh Leech

A technology of Phnom Penh leeches and protein peptides, which is applied in the field of protein peptide preparation, can solve the problems of certain risks in pollution and safety control, and achieve the effects of strong physiological functions, high separation rate, and fast absorption

Active Publication Date: 2017-09-29
深圳知本康业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of these processes involves the utilization of a large amount of protein after the hirudin is extracted, and conventional methods use a large amount of solvents, and there are certain risks in the control of pollution and safety.

Method used

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  • - A method for preparing protein peptides from Phnom Penh Leech

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Experimental program
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Effect test

Embodiment 1

[0043] A method for preparing protein peptides from Phnom Penh leech, including the following steps:

[0044] (1) Rinse the fresh leeks with clean water or thaw the pollutants on the body surface of the leeks, then transfer them into a potassium permanganate solution with a concentration of 3‰, stir and soak for 10 minutes; after picking it up, wash off the potassium permanganate solution with water, and then Transfer it into a 5% sodium chloride solution and stir and soak for 10 minutes. After picking it up, wash away the salt on its body surface with clean water, then rinse it with deionized water once, drain off the water, and place it in a container;

[0045] (2) After disinfecting and sterilizing the body surface of the leech, add 3‰ sodium bisulfite, 1‰ ascorbic acid and 3 times deionized water according to the weight of the leech, mix well, put it into a colloid mill for grinding, and control the grinding temperature at less than 50℃ , To obtain leech slurry; the fineness of...

Embodiment 2

[0059] A method for preparing protein peptides from Phnom Penh leech, including the following steps:

[0060] (1) Rinse the fresh leeks or thaw the pollutants on the body surface of the leeks with clean water, then transfer them into a potassium permanganate solution with a concentration of 3‰, stir and soak for 15 minutes; after picking up, wash off the potassium permanganate solution with water, and then Transfer it into a 5% sodium chloride solution and stir and soak for 10 minutes. After picking it up, wash away the salt on its body surface with clean water, then rinse it with deionized water once, drain off the water, and place it in a container;

[0061] (2) After disinfecting and sterilizing the body surface of the leech, add 3‰ sodium bisulfite, 1‰ ascorbic acid and 3 times deionized water to calculate the leech weight, mix well, put it into a colloid mill for grinding, and control the grinding temperature at less than 50°C , To obtain the leech slurry; the fineness of the ...

Embodiment 3

[0075] A method for preparing protein peptides from Phnom Penh leech, including the following steps:

[0076] (1) First rinse the fresh leeks or thaw the pollutants on the body surface of the leeks with clean water, then transfer them into a potassium permanganate solution with a concentration of 3‰, stir and soak for 12 minutes; after picking it up, wash off the potassium permanganate solution with water, and then Transfer it into a 5% sodium chloride solution and stir and soak for 10 minutes. After picking it up, wash away the salt on its body surface with clean water, then rinse it with deionized water once, drain off the water, and place it in a container;

[0077] (2) After disinfecting and sterilizing the body surface of the leech, add 3‰ sodium bisulfite, 1‰ ascorbic acid and 3 times deionized water according to the weight of the leech, mix well, put it into a colloid mill for grinding, and control the grinding temperature at less than 50℃ , To obtain leech slurry; the finen...

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Abstract

The invention belongs to the technical field of biological engineering, and specifically relates to a method for extracting protein peptide from poecilobdella manillensis. The method comprises the following steps: disinfection, grinding, coarse filtration, microfiltration, ultrafiltration, alkali dissolution, microwave treatment, centrifugation, precipitation, discoloring, enzymatic hydrolysis, precipitation, microfiltration, ultrafiltration, nano filtration, condensation, and spray drying. A physical method is used to replace a chemical method to separate acidic polypeptide so as to obtain a coarse product of acidic polypeptide; the coarse product can be used to produce Chinese patent medicine containing acidic polypeptide and purified acidic polypeptide, the separation rate is high, the activity can be well protected, no pollution is generated, and clean production is achieved.

Description

Technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to a method for preparing protein peptides from golden leeches. Background technique [0002] Leech, also known as leech, belongs to the class of Annelids. It has no bristles on the body surface and suckers on the front and back. It has developed body muscles and small body cavity. It is a highly specialized annelid. According to reports, there are more than 500 species of leeches in the world. They are divided into two categories: blood-sucking and non-blood-sucking. The majority of lees live in fresh water. They are also found at the intersection of salt water and brackish water, and a few live on land. The blood-sucking mountain lees live in the moist shrubs and grasses in the mountainous areas. In China, the larger individual is the wide-body golden leeches, with a body length of 30cm and a weight of 40g. However, a kind of appearance has recently been found in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30
Inventor 梁尚文梁满水林栋才曾志亮
Owner 深圳知本康业有限公司
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