A kind of freeze-dried preparation of nematode-predating fungus and its preparation method and application
A technology of freeze-drying preparations and vacuum freeze-drying, which is applied in nematicides, botanical equipment and methods, applications, etc., and can solve the problems of high viscosity, insignificant protection of chlamydospores, batch production of biocontrol agents, Standardization and standardization of effective content and other issues, to achieve the effect of cost saving and convenient implementation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0017] Embodiment 1: Preparation of lyophilized preparation of nematode-preying fungi
[0018] (1) Isolation, purification, isolation and identification of the nematode-predating fungus Arthrobotrys (Duddingtonia) flagrans isolate SDH 035
[0019] In August 2014, SDH035 was isolated from 5 samples collected from sheep manure piles in Wuzhong area, Ningxia, using the improved plate spreading method. During the separation, the third stage larvae of sheep nematodes were added to the plate as bait and incubated at 25°C. Or check under an inverted microscope every other day for 3 weeks, pick out a single predator structure or prey nematodes, transfer to 0.2% bran agar at 25°C for 3-14 days, and wait for conidia to grow , pick single conidia in another 0.2% bran agar plate until a pure culture is obtained. The morphology and data measurement of the conidia of this strain were obtained by the slide insertion method, and the DNA of this strain was extracted, and the primers ITS1 and ...
Embodiment 2
[0024] Embodiment 2: Germination rate experiment of freeze-dried preparation
[0025] Weigh an appropriate amount of freeze-dried preparation prepared in Implementation 1 and add it to 30ml sterilized distilled water to dilute it to 1.5×10 4 chlamydospores per ml, absorb 0.35~0.4ml and spread it on the water agar (WA) plate and incubate at 25~26°C. After 24 hours of incubation, use a puncher to take the agar culture and put it on a glass slide with cotton blue staining solution Staining, observe under the light microscope one by one to count the number of chlamydospores germinated and not germinated (directly place the plate for observation under an inverted microscope, it is difficult to obtain a more accurate counting result after testing), according to the formula for predicting the size of the sample: To predict the sample size, the total number of counted spores should be greater than >2000. In addition, after repeated tests, the germination rate of chlamydospores just ...
Embodiment 3
[0048] Embodiment 3: Insecticidal rate experiment of freeze-dried preparation
[0049] Dilute the lyophilized preparation with sterile distilled water to 1 × 10 6 / ml suspension of chlamydospores, take by weighing the fresh feces 10g of the artificially infected Haemonchus contortus and Trichostrongylus serpentine sheep during the test and put it into a petri dish with a diameter of 4.5cm, then add 1ml of test bacterium liquid to make Mix it with feces evenly, add appropriate amount of distilled water and sawdust according to the dry humidity of feces, put the small plate into a larger plate with a diameter of 9 cm, and add 2 ml of distilled water. The experimental group was paralleled three times, and the same number of plates without fungal spores was set up as the control group, cultured in the dark at 26°C for 12 days, and then the three-stage larvae (L3) were collected by the Bellman method and L3 counted. The above experiments were repeated twice.
[0050]
[0051] ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More