Numerically-controlled machine tool capable of preventing illegal operation
A CNC machine tool, illegal operation technology, applied in metal processing machinery parts, maintenance and safety accessories, metal processing equipment, etc., can solve the problems of misoperation, identification of operators, low safety, etc., to achieve fast computing speed, high safety The effect of sex, high security
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Embodiment 1
[0115] Example 1 Constructing a microfluidic chip detection system:
[0116] Microfluidic chip detection system, the detection device of the system is a microfluidic chip, which includes:
[0117] Eight-channel automatic syringe pump mixer area, sensor signal detection area and liquid outlet area;
[0118] The nano-sensor detection solution and sample solution are added to the microfluidic chip, and the eight-channel automatic syringe pump is used as the power to enter the liquid into the micro-mixer area through the micro-channel;
[0119] The micro-mixer area is mainly used for the full mixing of the nano-sensor detection liquid and the sample, and enters the sensing signal detection area after the mixing is complete;
[0120] The sensing signal detection area is mainly used for the detection of the fluorescent signal generated after the nanosensor detects the target gene, and enters the liquid outlet area after the detection is completed;
[0121] The detected samples and...
Embodiment 2
[0132] Example 2 Preparation of the detection solution and molecular probes used in the detection area of the system:
[0133] The detection solution used in the detection area of the system includes the concentration of aminated graphene oxide and molecular probes, and also includes POSSFF; more preferably, it also includes 0.5g / L malic acid.
[0134] The present invention determines the optimal composition of nano-biosensor aminated graphene oxide and fluorescent molecular probe, increases the amino group, thereby increasing the water solubility of the organic carrier, and increasing the negative charge on the surface of the carrier, so that the single-stranded DNA molecular probe The combination with the carrier is more stable, increasing the sensitivity and stability of the reaction.
[0135] POSSFF is used in the detection solution to improve the detection limit of the detection solution. POSSFF (Blue‐fluorescentconjugated‐oligomersubstituted polyhedraloligomeric sils...
Embodiment 3
[0144] Example 3 The detection process of genes based on microfluidic chips:
[0145] The detection solution and the sample solution are simultaneously added to the microfluidic chip injection area through the microflow pump from different injection ports; the two solutions are fully mixed through the micro mixer, and the detection is performed after incubation for 20 minutes; after the detection, the waste liquid is passed through The sample outlet is discharged to complete the entire detection process. Fluorescence signals were collected and recorded by the CCD photosensitive device of the fluorescence microscope, and the quantitative data of the fluorescence light intensity were obtained after being processed by the image processing software ImageJ.
[0146] Carry out routine saliva, sweat, blood detection with described system, its detection limit is shown in table 2 below:
[0147] Table 2
[0148]
sweat
the blood
sample 1
8
...
PUM
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