Method for preparing beta-cyclodextrin-gold nano-particles and method for detecting cholesterol
A gold nanoparticle and cyclodextrin technology, which is applied in nanostructure manufacturing, nanotechnology, nanotechnology, etc., can solve the problems of restricting the application of gold nanoparticles, complicated and cumbersome post-processing, etc.
Inactive Publication Date: 2016-03-23
ANHUI NORMAL UNIV
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Problems solved by technology
Although the above two types of methods can synthesize β-cyclodextrin-modified gold nanoparticles, they are either cumbersome or require the addition of additives, resulting in complicated post-processing, which greatly limits the production of β-cyclodextrin-modified gold nanoparticles. particle application
Method used
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Embodiment 1
[0047] 1) Add 5 mL of 0.1 mol / L phosphate buffer solution (pH=7) to 35 mL of ultrapure water, then add 1 mL of 0.01 mol / L chloroauric acid trihydrate solution and 10 mL of 0.01 mol / L β-ring Stir the dextrin solution vigorously at 100°C until the reaction system gradually turns wine red;
[0048] 2) The above reaction system was centrifuged at 6000 rpm for 5 minutes, and then the lower layer was removed to obtain β-cyclodextrin-gold nanoparticles A1.
Embodiment 2
[0050] β-cyclodextrin-gold nanoparticles A2 were prepared according to the method of Example 1, except that the amount of β-cyclodextrin was 2 mL.
Embodiment 3
[0052] β-cyclodextrin-gold nanoparticles A3 were prepared according to the method of Example 1, except that the amount of β-cyclodextrin was 10 mL.
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Abstract
The invention discloses a method for preparing beta-cyclodextrin-gold nano-particles and a method for detecting cholesterol. The method for preparing the beta-cyclodextrin-gold nano-particles includes enabling water, gold source solution and beta-cyclodextrin solution to carry out contact reaction in the presence of buffer solution to obtain the beta-cyclodextrin-gold nano-particles. The gold source solution can be one or a plurality of types of chloroauric acid trihydrate solution, chloroaurate solution, tetra-nitroxyl alloy acid solution and tetra-nitroxyl alloy salt solution. The methods have the advantages that the beta-cyclodextrin-gold nano-particles can be prepared at one step, raw materials of the beta-cyclodextrin-gold nano-particles are easily available, and post-treatment is simple; the cholesterol can be sensitively and quantitatively detected by the aid of the beta-cyclodextrin-gold nano-particles, and the beta-cyclodextrin-gold nano-particles are excellent in anti-interference capacity.
Description
technical field [0001] The invention relates to β-cyclodextrin-gold nanoparticles, in particular to a method for preparing β-cyclodextrin-gold nanoparticles and a method for detecting cholesterol. Background technique [0002] Nano-gold particles with diameters at the nanometer scale, whose basic units are tiny-sized particles, have many physical properties that macro-particles do not have, such as optical effects, small-size effects, surface effects, macroscopic quantum tunneling effects, and dielectric limitations. domain effect, Kubo effect, and some other special effects. These effects make gold nanoparticles widely used in materials, medical testing, clinical medicine, food, chemical industry, ceramics, dyes and other fields. [0003] Gold nanoparticles with a supramolecular structure (ie, gold nanoparticles modified by macromolecular substances) have both the characteristics of gold nanoparticles and macromolecular substances, and are currently widely used in the fiel...
Claims
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IPC IPC(8): B82B3/00B82Y30/00B82Y40/00G01N21/64
CPCB82B3/0019B82Y30/00B82Y40/00G01N21/6486
Inventor 夏云生赵燕顾文亮鹿淋淋
Owner ANHUI NORMAL UNIV
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