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Preparation and application of a highly sensitive lateral flow chromatography immunoassay test paper

A lateral flow chromatography and immune detection technology, applied in the field of biomedical detection, can solve the problems of limiting the sensitivity of test strips, random fluctuations, etc., and achieve the effects of high-sensitivity detection, good repeatability, and high-sensitivity quantitative detection.

Active Publication Date: 2022-03-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These non-specific adsorption signals have nothing to do with the concentration of the analyte and will fluctuate randomly, which greatly limits the sensitivity of the test paper
At present, there is no test paper system based on the above gap-enhanced Raman probe that can solve the problem of non-specific adsorption and further improve the sensitivity

Method used

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  • Preparation and application of a highly sensitive lateral flow chromatography immunoassay test paper
  • Preparation and application of a highly sensitive lateral flow chromatography immunoassay test paper
  • Preparation and application of a highly sensitive lateral flow chromatography immunoassay test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Preparation of core-shell gold nanoparticles embedded with 4,4'-biphenyldithiol (4,4'-BPDT) molecules

[0039] 20mL, 1nM 20nm gold particles were mixed with 1mL, 10mM 4,4'-biphenyldithiol for 19 hours, then centrifuged and dispersed in 20mL, 0.05M cetyltrimethylammonium chloride solution Then take 1mL of the above-mentioned adsorbed product, mix and shake with 20mL, 0.05M cetyltrimethylammonium chloride, 1mL, 5mM chloroauric acid and 800μL, 0.04M ascorbic acid, to obtain the core-shell structure embedded 1, Gold nanoparticles of 4-benzenedithiol molecules.

Embodiment 2

[0040] Example 2: Preparation of core-shell gold nanoparticles embedded with 4-nitrobenzenethiol (4-NBT) molecules

[0041] 20mL, 1nM 20nm gold particles were mixed with 1mL, 10mM 4-nitrobenzenethiol for 10 minutes, then centrifuged and dispersed in 20mL, 0.05M cetyltrimethylammonium chloride solution, and then Take 1mL of the above-mentioned adsorbed product, mix and shake with 16mL, 0.05M hexadecyltrimethylammonium chloride, 800μL, 5mM chloroauric acid and 500μL, 0.04M ascorbic acid, to obtain 4-nitrobenzene embedded in the core-shell structure Gold nanoparticles of thiol molecules.

Embodiment 3

[0042] Example 3: Preparation of gold nanorod particles with 1,4-benzenedithiol (1,4-BDT) molecules adsorbed on the surface

[0043] Step 1: Mix 5mL, 0.45mM chloroauric acid solution with 5mL, 0.2M hexadecyl ammonium bromide, add 0.5mL, 0.01M frozen sodium borohydride solution, stir for 2 minutes, and stand for 2 hours to obtain gold species solution.

[0044] Step 2: Mix 5 mL, 0.2M cetyl ammonium bromide, 5 mL, 1 mM chloroauric acid solution, 0.5 mL, 1 mM silver nitrate and 0.08 mL, 0.1 M ascorbic acid, and stir for 2 minutes.

[0045] Step 3: Add 0.012 mL of the gold seed solution obtained in Step 1 to the solution obtained in Step 2, stir for 2 minutes, and stand at room temperature for 3 hours to obtain a gold nanorod solution.

[0046] Step 4: Take 20mL, 1nM of the gold nanorod solution obtained in Step 3, mix and adsorb with 1mL, 10mM 1,4-benzenedithiol for 27 hours, and wash by centrifugation to obtain the gold nanorod solution with 1,4-benzenedithiol adsorbed on the s...

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Abstract

The invention discloses the preparation and application of a high-sensitivity lateral flow chromatography immunoassay test paper, relates to the technical field of biomedical detection, and includes a binding pad, a dual-probe calibration system and a detection line, and the binding pad is provided with the double-probe Needle calibration system, the detection line is used to measure the Raman signal, the dual-probe calibration system includes a signal surface enhanced Raman probe and a calibration surface enhanced Raman probe, the calibration surface enhanced Raman probe is used for Signal calibration is performed on the signal surface-enhanced Raman probe. The probe prepared by the invention has strong signal, good stability, high sensitivity of the dual-probe calibration system, strong anti-interference ability and good repeatability, and realizes the highly sensitive and repeatable quantitative detection of target objects on the test paper base.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to the preparation and application of a high-sensitivity lateral flow chromatography immunoassay test paper. Background technique [0002] Lateral flow chromatography immunoassay test paper is a paper-based detection method, which is widely used in timely on-site detection. Its advantages are short detection time, convenient use, low cost and easy storage. Lateral flow chromatography immunoassay test paper is generally composed of the following parts: substrate, sample pad, binding pad, reaction membrane and water-absorbing pad. Among them, the reaction membrane is generally a nitrocellulose membrane with a detection line and a control line on it. The corresponding antibody is carried on the line, and there will be a label coupled with the antibody on the binding pad. Traditional markers are red colloidal gold nanoparticles. The surface of this gold nanoparticle is mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/532G01N21/65
CPCG01N33/558G01N33/532G01N21/658
Inventor 叶坚徐宏谭自扬古宏晨
Owner SHANGHAI JIAO TONG UNIV
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