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A method for rapid detection of exosomes by SERS signaling

A technology of exosomes and signals, which is applied in the field of rapid detection of exosomes, can solve the problems of complex sample processing and time-consuming detection process, and achieve the effects of sensitive quantitative detection, improved reliability, and simplified steps

Active Publication Date: 2019-04-09
SOUTHEAST UNIV
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  • Abstract
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Problems solved by technology

[0005] Purpose of the invention: In order to overcome the deficiencies in the prior art, the present invention provides a method for rapid detection of exosomes through SERS signals, through Raman probes, magnetic capture substrates and immune recognition, to realize the detection of exosomes in samples. Rapid, high-sensitivity quantitative analysis can solve the problems of complex sample processing and time-consuming detection process in the prior art

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  • A method for rapid detection of exosomes by SERS signaling
  • A method for rapid detection of exosomes by SERS signaling

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Embodiment Construction

[0015] The present invention will be further described below in conjunction with the accompanying drawings. The PBS buffer solution involved in the examples has a pH of 7.4 and a concentration of 10 mM.

[0016] With 5,5-dithiobis(2-nitrobenzoic acid) (DTNB) as the Raman molecule and the exosomes of human cervical cancer cells (HeLa) as the exosomes to be tested, the method of the present invention is used Exosome detection experiments were carried out.

[0017] Step 1, preparation of iron ferric oxide nanoparticles

[0018] Ferric oxide nanoparticles were prepared by solvothermal method. Add 2.7g ferric chloride hexahydrate (FeCl 3 ·6H 2 (2), 1g polyethylene glycol (PEG, molecular weight 200) and 3.6g sodium acetate, stir half an hour to make it fully mix. Subsequently, the mixed solution was charged into a polytetrafluoroethylene reactor at 200° C. for 8 hours. The product is magnetically separated and washed repeatedly with deionized water to obtain ferric chloride nan...

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Abstract

The invention discloses a method for quickly detecting exosomes through an SERS (Surface Enhanced Raman Scattering) signal. The method comprises the following steps: firstly, identifying the exosomes in a sample through an immune response by utilizing a magnetic capture substrate and a Raman microprobe to form a sandwich structure in the form of magnetic capture substrate-exosome-Raman microprobe; then, separating a collected product by utilizing an externally applied magnetic field; finally, analyzing the SERS signal of the product by utilizing a confocal Raman microscopy system, wherein the SERS signal is stronger, and the concentration of the exosomes is higher. According to the method, the SERS technology is applied to detecting the exosomes; precise qualitative and quantitative analysis is performed on the sample by adopting the SERS technology, and quick and high-sensitivity detection of the exosomes can be realized.

Description

technical field [0001] The invention relates to a method for rapidly detecting exosomes through SERS signals, which belongs to exosome detection technology. Background technique [0002] Exosomes are small exocrine vesicles with a lipid bilayer membrane structure and a diameter of about 40-100 nm. Although exosomes were discovered as early as 1983, they were long thought to be just waste products of cells. In recent years, it has been discovered that these tiny membrane vesicles contain cell-specific proteins, lipids and nucleic acids, which can be used as signal molecules to be transmitted to other cells to change the function of other cells. Recent studies have found that exosomes play an important role in physiological and pathological phenomena, such as antigen presentation in immunity, tumor growth and migration, and repair of tissue damage. Exosomes secreted by different cells have different components and functions and can be used as biomarkers for disease diagnosis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 宗慎飞王乐陈晨王著元崔一平
Owner SOUTHEAST UNIV
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