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Method for extracting sargassum thunbergii polysaccharide

A technology of sargassum polysaccharides and extraction methods, which is applied in the field of extraction of sargassum polysaccharides, can solve the problems of time-consuming, low yield, energy consumption, etc., and achieve strong cancer cell growth inhibition, high yield, and strong resistance to cancer cells. The effect of oxidation activity

Inactive Publication Date: 2016-03-23
HUAQIAO UNIVERSITY
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Problems solved by technology

[0004] Therefore, aiming at the above content, the present invention provides a method for extracting sargassum polysaccharides, which solves the problems of time-consuming, energy-consuming and low-yield extraction methods in the prior art

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Embodiment Construction

[0015] The implementation of the present invention will be described in detail below in conjunction with specific examples, so as to fully understand and implement the process of how to apply technical means to solve technical problems and achieve technical effects in the present invention.

[0016] Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the reagents and products used are also commercially available. The sources, trade names and, where necessary, listing of constituents of reagents used are indicated the first time they appear.

[0017] Embodiments of the invention are:

[0018] A method for extracting sargassum polysaccharides, comprising the following processing steps:

[0019] a. Constant temperature water bath treatment: Sargassum and dilute acid solution (adjust pH=2 with hydrochloric acid solution) with a material-to-liquid ratio of 1:120, bathe in a constant temperature wat...

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Abstract

The invention relates to the technical field of sargassum thunbergii polysaccharide extraction, provides a method for extracting sargassum thunbergii polysaccharide and solves the problems that in the prior art, the extraction methods consume time and energy and are low in yield. The method comprises the following processing steps: 1, constant temperature water bath treatment; 2, pre-treatment of an extracting solution, wherein the extracting solution is concentrated and cooled to the room temperature, anhydrous ethanol with the volume four times the volume of the extracting solution is added for alcohol precipitation, the mixture is placed in a refrigerator with the temperature kept at 4 DEG C for a night, centrifugation is conducted, supernatant is discarded, distilled water is added for dissolution and sedimentation, repeated freezing-thawing is conducted on an obtained solution, centrifugation is conducted, impurities are removed, the freezing condition is minus 20 DEG C, the dissolving condition is 50 DEG C, the operation is repeated seven times, then a freeze-drying machine is adopted for drying, and a crude product (1) is obtained; 3, deproteinization of the crude product (1); 4, separation and purification of an obtained crude product (2).

Description

technical field [0001] The invention relates to the technical field of extracting seaweed polysaccharides, in particular to a method for extracting sargassum polysaccharides. Background technique [0002] Sargassum belongs to the family of Entomia, Fucusaceae, Sargassumaceae, and Sargassum genus. It grows on rocks or in bogs in the mid-tidal zone. It is widely distributed in coastal areas of my country and can be cultivated on a large scale. It has rich raw materials and is harvested Contained in "Chinese Marine Drug Dictionary". Because of its functions of softening firmness and dispelling stagnation, diuresis and swelling, clearing away heat and resolving phlegm, and having comprehensive nutritional value, it has been used in medicine, health care, aquaculture and chemical industry, and has many potentials to be developed. [0003] Sargassum is rich in Sargassum polysaccharides, which can promote the release of superoxide anion free radicals in human granulocytes and incre...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00A61P39/06
CPCC08B37/0003C08B37/00
Inventor 罗巅辉王昭晶袁秀梅曾亚威聂开颖李志明
Owner HUAQIAO UNIVERSITY
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