Water-permeable brick for pavement
A technology of permeable bricks and bricks, applied in the field of road surface purification, can solve the problems of loss of permeable function, shortened service life, cracking of brick surface, etc., and achieve the effect of improving water quality, reducing pollution and preventing odor
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[0035] In the present invention, the preparation method of the microbial inoculum can be well known to those skilled in the art, for example, it can include: separately inoculating Bacillus subtilis, Rhodopseudomonas palustris, Bacillus licheniformis and Lactobacillus plantarum into the culture medium. to cultivate. Among them, Bacillus subtilis, Rhodopseudomonas palustris, Bacillus licheniformis, and Lactobacillus plantarum can be cultured in separate culture systems.
[0036] Specifically, the method for culturing Bacillus subtilis (Bacillus subtilis) according to the present invention is not particularly limited, and is well known to those skilled in the art. For example, Bacillus subtilis can be first cultivated in a seed medium (LB medium). Density OD 600 The value is 0.6-0.8, to obtain the bacterial liquid, and then inoculate 2-5 weight parts of Bacillus subtilis in 100 weight parts of production medium (glucose 5g, soybean meal 30g, peptone 2g, distilled water 1000ml, pH7....
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[0053] Example 1
[0054] This embodiment is used to illustrate the microbial inoculum of the present invention and the filtering device 3 of the permeable brick for pavement using the inoculum.
[0055] (1) Inoculate 5 parts by weight of Bacillus subtilis broth (purchased from China Agricultural Microbial Culture Collection) in 100 parts by weight of medium (5 g glucose, 30 g soybean meal, 2 g peptone, 1000 ml distilled water, pH 7.0-7.2) And the product number is CFCC10442, cultivated to the bacteria density OD in the same LB medium as above 600 The value is 0.6-0.8, and the bacterial solution is obtained), cultivated at 37°C, samples are taken during the cultivation process and observed by direct counting with a microscope, until the number of viable Bacillus subtilis is 10 8 cfu / gram.
[0056] (2) Inoculate 5 parts by weight of Bacillus licheniformis (purchased) in 100 parts by weight of the culture medium (0.45g of corn steep liquor, 1.50g of peptone, 1:15 of soybean meal powder...
Example Embodiment
[0061] Example 2
[0062] This embodiment is used to illustrate the microbial inoculum of the present invention and the filtering device 3 of the permeable brick for pavement using the inoculum.
[0063] According to the method of Example 1, the Bacillus subtilis obtained in steps (1) to (4) were mixed with Rhodopseudomonas palustris, Bacillus licheniformis and Lactobacillus plantarum according to the proportions. The difference is that the mixing ratio In the resulting microbial inoculum, the cfu ratio of Bacillus subtilis to Rhodopseudomonas palustris, Bacillus licheniformis and Lactobacillus plantarum is 1:0.8:0.1:0.3. The obtained mixed bacterial liquid is vacuum dried, pulverized, and passed through a 40-mesh sieve to form a solid bacterial agent, which is placed in a permeable brick filter barrel 31 together with quartz sand with a particle size of 0.8-1.8 mm, and the top plate 32 is covered. , The height of the quartz sand filler layer is 118mm, and the amount of bacteria a...
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