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Preparation method and application of biological nanopatch

A bio-nano and patch technology, applied in the fields of biology and medicine, can solve problems such as the failure to find repair materials

Active Publication Date: 2016-03-30
HANGHZOU HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the application of biological mesh in the repair of abdominal wall hernia and abdominal wall defect has become a research hotspot, but so far no ideal repair material has been found in the world.

Method used

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  • Preparation method and application of biological nanopatch
  • Preparation method and application of biological nanopatch
  • Preparation method and application of biological nanopatch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1, a kind of polylactic acid-glycolic acid nanoemulsion (PLGA) preparation method, carries out following steps successively:

[0096] 1) At room temperature, dissolve 0.1g PLGA in 16ml of acetone: ethanol (V / V) = 3:1 organic phase (organic solvent I) to obtain an organic solution (colorless transparent liquid); 0.4g poloxamer 188 It was dissolved in 40 ml of deionized water under 4 cm magneton and 800 rpm / s stirring to obtain an aqueous solution (colorless transparent liquid).

[0097] 2) At room temperature, slowly drop the organic solution obtained in step 1) into the aqueous solution at a speed of 800 rpm / s with a 4cm magnet (30-60 minutes). After the dropwise addition, continue to stir for 30 min to evaporate most of the organic solvent to obtain an opalescent nano-suspension.

[0098] 3) The opalescent nano-suspension obtained in step 2) was rotary-evaporated with a rotary evaporator for 120 minutes at 37° C. to remove residual organic solvent and part ...

Embodiment 2

[0099] Embodiment 2, a kind of polylactic acid-glycolic acid nanoparticle (PLGA) preparation method, carries out following steps successively:

[0100] 1), at room temperature, get 0.1g PLGA and dissolve in 16ml acetone: ethanol (V / V)=3:1 in the organic phase (organic solvent) to obtain organic solution (colorless transparent liquid); 0.4g poloxamer 188 in 2cm magneton, dissolved in 40ml deionized water under stirring at 800rpm / s to obtain an aqueous solution (colorless transparent liquid).

[0101] 2) At room temperature, slowly drop the organic solution obtained in step 1) into the aqueous solution under a 2cm magnet and a stirring speed of 800rpm / s (30-60 minutes). After the dropwise addition, continue to stir for 30 min to evaporate most of the organic solvent to obtain an opalescent nano-suspension.

[0102] 3) The nano-suspension with opalescence obtained in step 2) was rotary-evaporated with a rotary evaporator for 120 min at 37° C. to remove residual organic solvent a...

Embodiment 3

[0103] Embodiment 3, a kind of polylactic acid-glycolic acid nanoparticle (PLGA) preparation method, carries out following steps successively:

[0104] 1), at room temperature, take 0.1g PLGA and dissolve in 8ml of acetone: ethanol (V / V) = 3:1 in the organic phase to obtain an organic solution (colorless transparent liquid); Dissolve in 40 ml of deionized water under stirring at 800 rpm / s to obtain an aqueous solution (colorless transparent liquid).

[0105] 2) At room temperature, slowly drop the organic solution obtained in step 1) into the aqueous solution under a 2cm magnet and a stirring speed of 800rpm / s (30-60 minutes). After the dropwise addition, continue to stir for 30 min to evaporate most of the organic solvent to obtain an opalescent nano-suspension.

[0106] 3) The opalescent nanosuspension obtained in step 2) was evaporated with a rotary evaporator for 90 minutes at 37° C. to remove residual organic solvent and part of water to obtain a polymer colloidal suspen...

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Abstract

The invention discloses a method for preparing a biological nanopatch constructed by astragaloside induced BMSC compound polylactic acid-glycolic acid nano-particle modified small intestinal submucosa matrix. The method comprises the following steps: preparing polylactic acid-glycolic acid nano-particles (PLGA) by adopting a multiple emulsion method; preparing a small intestinal submucosa matrix (SIS) having a natural three-dimensional ultra micro-structure by adopting a decellation technology to adsorb the PLGA nano-particles on the surface of SIS and favor adsorption and growth of host cells by means of the SIS after PLGA nano-particle surface modification; inducing BMSC by astragaloside to promote proliferation and differentiation of BMSC and enable the BMSC to compound and grow on the PLGA nano-particle surface modified small intestinal submucosa matrix (PLGA-SIS) of pigs. Therefore, the AS-BMSC-PLGA-SIS biological patch has stable physicochemical property, good biocompatibility and high mechanical strength, and is beneficial to tissue regeneration.

Description

technical field [0001] The invention belongs to the technical field of biology and medicine, and relates to material chemistry, specifically, through decellularization technology, synthesizes astragaloside IV with good biocompatibility to induce BMSC composite polylactic acid-glycolic acid nanoparticles to modify the small intestinal submucosa matrix to construct bionano Patch (AS-BMSC-PLGA-SIS), the present invention also relates to the application of the biological nanopatch in medicine. Background technique [0002] Abdominal wall hernia, abdominal wall defect caused by trauma, tumor, infection, is a common disease in surgery, and its treatment is one of the most difficult clinical problems in surgery. The artificial repair materials used in clinical operations mainly rely on imports, which are expensive. Non-degradable polymer materials are currently the most used materials for abdominal wall repair and reconstruction. After implantation of non-absorbable polymer materi...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/18A61L27/54
Inventor 黄海陈亚倩刘殿雷李晓文
Owner HANGHZOU HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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