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Strain L252 capable of efficiently degrading lignocellulose substances and application of strain

A lignocellulose-based, high-efficiency degrading bacteria technology, applied in bacteria, microorganisms, microorganisms, etc., can solve the problem of low enzyme activity, and achieve the effects of stable enzyme activity, stable enzyme production, and rapid value-added.

Active Publication Date: 2016-04-06
北京中农国泰科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of fungi to degrade lignocellulose generally has the problem of low enzyme activity

Method used

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  • Strain L252 capable of efficiently degrading lignocellulose substances and application of strain
  • Strain L252 capable of efficiently degrading lignocellulose substances and application of strain
  • Strain L252 capable of efficiently degrading lignocellulose substances and application of strain

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specific Embodiment approach 1

[0024] Specific embodiment 1: A lignocellulosic substance degrading bacterium of this embodiment, it is Klebsiella pneumoniae (Klebsiellapneumoniae) L252, preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures, and the preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the city, the date of preservation is December 12, 2014, and the preservation number is CGMCCNO.10161.

[0025] The Klebsiella pneumoniae (Klebsiellapneumoniae) L252 of the present embodiment is a gram-negative bacterium, and the shape of the bacterial strain is rod-shaped, and the size of the bacterial cell is 0.57~0.62×1.4~1.5 μm, does not form spores, and has no flagella. Capsulated; forms round, opaque, milky-white colonies with smooth protrusions and neat edges on LB medium (eg figure 1 shown).

[0026] The results of the physiological and biochemical reactions of the strain included 18 items and 28 indicators; co...

specific Embodiment approach 2

[0087] Embodiment 2: The application of a lignocellulosic substance-degrading bacteria in this embodiment, which is used to degrade lignocellulosic substances.

[0088] Bacterial strain of the present invention is carried out following functional detection:

[0089] The Klebsiella pneumoniae (Klebsiellapneumoniae) L252 of the specific embodiment was tested for the weight loss rate and lignin, cellulose and hemicellulose degradation of bagged fungus waste and corn stalks to verify its unique functions. details as follows:

[0090] 1. Determination of weight loss rate of bagged fungus waste and corn stalks

[0091] 1.1 Corn stalk powder

[0092] Corn stalks were taken from the experimental field of the laboratory in Hulan Campus, Heilongjiang University, Harbin City, Heilongjiang Province in October 2012, dried, crushed and passed through a 40-mesh sieve, and set aside.

[0093] 1.2 bags of fungus waste

[0094] Bags of fungus waste were provided by Mudanjiang Branch of Heil...

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Abstract

The invention relates to a strain L252 capable of efficiently degrading lignocellulose substances and application of the strain. The stain is Klebsiella pneumoniae L252 which is preserved in China General Microbiological Culture Collection Center on Dec. 12th, 2014, and the preservation number is CGMCC No.10161. The strain is used for degrading the lignocellulose substances. The strain can be proliferated rapidly, the adaptability is high, and application is wide. The weight-loss ratio of bagged agaric waste subjected to fermentation treatment of the Klebsiella pneumoniae L252 is 30% or above, and the weight-loss ratio of maize straw subjected to fermentation treatment of the Klebsiella pneumoniae L252 is 26% or above.

Description

technical field [0001] The invention relates to a high-efficiency degradation bacterium L252 of lignocellulosic substances and its application. Background technique [0002] my country is a large agricultural country, and the amount of agricultural waste generated is extremely huge. Due to the limitations of economic benefits and technology popularization, most of the agricultural waste is extensively and inefficiently used and the state of idleness is serious, resulting in waste of resources and environmental pollution. Waste has become China's largest source of pollution. However, there is a large amount of lignocellulose in agricultural waste, which needs to be developed and utilized urgently. [0003] Lignocellulose is the second most abundant organic renewable resource after cellulose in nature, and it is also one of the most difficult components for microorganisms to degrade. In recent years, some lignin-degrading fungi have begun to be applied in practice, but furthe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/22
CPCC12N1/205C12R2001/22
Inventor 杨微刘春光杨峰山孙永帅吴奇王鲲鹏王庆华魏才强张慧曲金玲于文全
Owner 北京中农国泰科技有限公司
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