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Composition and application containing bacillus subtilis and sclerotin

A technology of bacillus subtilis and sclerotium netting, applied in the field of pesticides, can solve the problems of decreased control effect, excessive pesticide residues, enhanced drug resistance of pathogenic bacteria, etc., and achieves the effect of reducing the amount of use, synergistic effect, and reducing the risk of drug resistance

Active Publication Date: 2019-01-11
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The first purpose of the present invention is to solve the problems that a large number of chemical pesticides are used in the current production to prevent and control rape sclerotinia, serious environmental pollution, excessive pesticide residues, decline in rapeseed quality, and enhanced drug resistance of pathogenic bacteria, resulting in decreased control efficiency and even failure of control. A kind of subtilis that has good control effect and synergistic effect on rape sclerotia, not only can play the role of Bacillus subtilis Pc3 to effectively control rape sclerotinia, but also can reduce the risk of existing pathogenic bacteria's potential resistance to sclerotia net Composition and application of bacillus and sclerotin

Method used

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  • Composition and application containing bacillus subtilis and sclerotin
  • Composition and application containing bacillus subtilis and sclerotin
  • Composition and application containing bacillus subtilis and sclerotin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Preparation of Bacillus subtilis Pc3 strain spore preparation

[0015] Preparation process: first insert Pc3 into nutrient agar medium and shake culture (200r / min, 28℃) for 48h, treat in a water bath at 80℃ for 15min, centrifuge at 8000r / min for 15min, remove the supernatant to obtain bacterial precipitation, add 10mL to filter and sterilize MgSO 4 Solution (0.lmol / L) and 1mL sterile glycerin, shake and mix, and then add 1mL of 1.5% (w / v) sodium alginate solution, and then mix the above mixture with 50g talc and 4g Ca-lignosulphate. Mix and store at room temperature under sterile conditions. The number of live spores of Bacillus subtilis Pc3 spore preparation is 10 10 cfu / g.

Embodiment 2

[0016] Example 2: Preparation of 20% wettable powder (each component is by mass)

[0017]

Embodiment 3

[0018] Example 3: The control effect of 20% wettable powder against sclerotinia sclerotiorum of potted rape (%)

[0019] Inoculate Rapeseed Sclerotinia sclerotiorum into the center of the PDA plate, and place it in an incubator at 25°C for 3 days for use.

[0020] The ratio of Bacillus subtilis Pc3 (10 billion live spores / g) to Sclerotium 1:1 (mass ratio) is formulated into a 20% wettable powder, and Bacillus subtilis Pc3 and Sclerotin are separately formulated into the above wettable powder For comparison, spray the prepared rapeseed sclerotinia sclerotiorum into a plate with a diameter of 0.5cm after 24 hours to inoculate the middle of the rape leaves, each plant with 4 leaves, 10 rapeseed, and absorbent cotton on the hyphae block for moisture retention , After 5 days of inoculation, the incidence index was investigated, and the control effect was calculated. The results of the control effect of 20% Bacillus subtilis Pc3·Sclerotium WP on potted rape sclerotia are shown in Table 1...

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Abstract

The invention provides a composition containing bacillus subtilis and dimethachlon and application thereof and belongs to the field of pesticides. The composition containing bacillus subtilis Pc3 and dimethachlon takes bacillus subtilis Pc3 and dimethachlon as active components and the mass ratio of bacillus subtilis Pc3 to dimethachlon ranges from (1000:1) to (1:1000). The quantity of live spores in bacillus subtilis Pc3 can be 0.01-100 billion per gram. Wettable powder, suspension emulsion or emulsion in water can be prepared by adding a pesticide auxiliary agent to the composition containing bacillus subtilis Pc3 and dimethachlon. For example, the pesticide auxiliary agent is added to the active components including bacillus subtilis Pc3 and dimethachlon and the pH value is adjusted to be 5-7, and then the wettable powder is prepared. Dimethachlon can adopt an active compound and bacillus subtilis can adopt raw powder. The composition containing bacillus subtilis and dimethachlon can be applied to preparation of pesticides for preventing and treating sclerotinia rot of colza.

Description

Technical field [0001] The invention belongs to the field of pesticides, and specifically relates to a composition containing Bacillus sp. Pc3 and Sclerotium isolated from Antarctica and its application in the prevention and treatment of rape sclerotia. Background technique [0002] Rapeseed sclerotinia caused by Sclerotinia sclerotiorum is one of the important diseases in rape production. The annual disease rate is 10%-30%, and the serious disease can reach more than 80%. The diseased plant generally reduces the yield by 10%~ 70%. Rapeseed is one of the oil crops with the highest oil production efficiency. At present, the main control measures for rape sclerotinia disease are chemical control, agricultural control and biological control. But so far, no high-efficiency antibacterial nuclear disease varieties have been found. Therefore, chemical control methods are mainly used for the prevention and control of the disease, and benzimidazole fungicides are the most used. With the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01N37/32A01P3/00
CPCA01N37/32A01N63/00A01N2300/00
Inventor 陈新华陈雨郭文斌
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION