Petroleum-degrading bactericide and preparation method thereof

A technology of oil-degrading bacteria and bacillus, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as pollution, achieve environmental safety, convenient transportation and storage, and strong environmental adaptability

Active Publication Date: 2016-04-13
NORTH CHINA SEA ENVIRONMENTAL MONITORING CENT OF STATE OCEANIC ADMINISTATION
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies in the prior art, and provide a kind of petroleum degrading bacterial agent and preparation method thereof, ai

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Petroleum-degrading bactericide and preparation method thereof
  • Petroleum-degrading bactericide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example 1

[0034] A method for preparing petroleum degrading bacteria agent, including the following steps:

[0035] (1) Carry out the expansion culture to the strains of Caulobacter inert, Candida parapsilosis, Brevibacterium phenol-resistant, Arthrobacter sphaeroides, and Sporobolomyces salmonicola to a cell concentration of 10 9 ~10 10 cfu / g, mix inert bacillus, Candida parapsilosis, Brevibacterium phenolic resistant, Arthrobacter sphaeroides and Sporobolomyces salmonicola according to the mass ratio of 3:2:2:1:1 to obtain mixed bacteria Species

[0036] (2) Autoclave the liquid fermentation medium for 15-30 minutes, inoculate the sterilized liquid fermentation medium in an aseptic operation table, inoculate the mixed strain obtained in step (1), mix the strain and the liquid fermentation medium The weight ratio is 1:12, the liquid fermentation medium inoculated with mixed strains is placed on a constant temperature water bath shaker, and continuous fermentation is carrie...

Example Embodiment

[0043] Example 2

[0044] A method for preparing petroleum degrading bacteria agent, including the following steps:

[0045] (1) Carry out the expansion culture to the strains of Caulobacter inert, Candida parapsilosis, Brevibacterium phenol-resistant, Arthrobacter sphaeroides, and Sporobolomyces salmonicola to a cell concentration of 10 9 ~10 10 cfu / g, mix inert bacillus, Candida parapsilosis, Brevibacterium phenolic resistant, Arthrobacter sphaeroides and Sporobolomyces salmonicola according to the mass ratio of 3:2:2:1:1 to obtain mixed bacteria Species

[0046] (2) Autoclave the liquid fermentation medium for 15-30 minutes, inoculate the sterilized liquid fermentation medium in an aseptic operation table, inoculate the mixed strain obtained in step (1), mix the strain and the liquid fermentation medium The weight ratio is 1:10, the liquid fermentation medium inoculated with mixed strains is placed on a constant temperature water bath shaker, and continuous fermentation is carrie...

Example Embodiment

[0053] Example 3

[0054] A method for preparing petroleum degrading bacteria agent, including the following steps:

[0055] (1) Carry out the expansion culture to the strains of Caulobacter inert, Candida parapsilosis, Brevibacterium phenol-resistant, Arthrobacter sphaeroides, and Sporobolomyces salmonicola to a cell concentration of 10 9 ~10 10 cfu / g, mix inert bacillus, Candida parapsilosis, Brevibacterium phenolic resistant, Arthrobacter sphaeroides and Sporobolomyces salmonicola according to the mass ratio of 3:2:2:1:1 to obtain mixed bacteria Species

[0056] (2) Autoclave the liquid fermentation medium for 15-30 minutes, inoculate the sterilized liquid fermentation medium in an aseptic operation table, inoculate the mixed strain obtained in step (1), mix the strain and the liquid fermentation medium The weight ratio is 1:15, the liquid fermentation medium inoculated with mixed strains is placed on a constant temperature water bath shaker, and continuous fermentation is carrie...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a petroleum-degrading bactericide and also relates to a preparation method of the petroleum-degrading bactericide. The preparation method of the petroleum-degrading bactericide comprises the following steps: (1) performing enlage cultivation on such bacteria as caulobacter segnis, candida parapsilosis, brachybacterium phenoliresistens, arthrobacter globiformis and sporidiobolus salmonicolor till the concentration of the bacteria is 10<9>-10<10> cfu/g, and uniformly mixing according to the mass ratio of 3: 2: 2: 1: 1 to obtain a mixed culture; (2) fermenting the mixed culture in a liquid fermentation culture medium for 48-60 h; (3) carrying out centrifugal separation to obtain thick strain cells; (4) uniformly mixing the thick strain cells with a 0.9% NaCl solution and a sea mud leachate; (5) adding nutrient substances, and then preserving at 4 DEG C in a sealed manner. The petroleum-degrading bactericide is high in petroleum degradation rate.

Description

technical field [0001] The invention belongs to the technical field of petroleum degradation, and in particular relates to a petroleum-degrading bacterial agent; the invention also relates to a preparation method of the above-mentioned petroleum-degrading bacterial agent. Background technique [0002] With the increasing development of oil development, shipping and oil industry, marine oil spill accidents occur frequently, and oil pollution has brought serious harm to the marine environment and marine ecosystem, and can directly or indirectly affect the survival and sustainability of human beings. Continuous development. According to the United Nations Environment Program report, 2 to 20 million tons of oil flow into the sea every year, and oil pollution has become a worldwide pollution problem. The oil spill in the Gulf of Mexico in 2010 was one of the most serious marine pollution incidents in the history of the United States, and the ecological damage and economic losses...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N1/16B09C1/10C12R1/72C12R1/06C12R1/01C12R1/645
Inventor 赵升韩龙江耿晓潘玉龙刘欣禹周瑞佳
Owner NORTH CHINA SEA ENVIRONMENTAL MONITORING CENT OF STATE OCEANIC ADMINISTATION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap