Preparation method of instant tea powder
A technology of instant tea powder and tea leaves, which is applied in the field of food processing, can solve the problems of continuous production of instant tea, difficulty in supply of enzyme preparations, small types and quantities, etc., and achieve no toxins, low price, and short fermentation cycle Effect
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Embodiment 1
[0030] (1) Source of bacteria
[0031] Aspergillus niger: purchased from China General Microorganism Culture Collection Management Center, strain preservation number: CGMCC3.316;
[0032] Aspergillus oryzae: purchased from China General Microorganism Culture Collection Management Center, strain preservation number: CGMCC3.863.
[0033] (2) Activation of bacteria
[0034] The species of Aspergillus niger and Aspergillus oryzae were inoculated on Chapeauer's agar medium for activation. Cha's agar medium: 30.0g sucrose, NaNO 3 3.0g, MgSO 4 ·7H 2 O7.5g, KCl0.5g, FeSO 4 4H 2 O0.01g, K 2 HPO 4 1.0g, 15.0g agar, 1L distilled water, pH6.0-6.5. Activation conditions: 24-48h activation culture at 28°C.
[0035] (3) Preparation of semi-finished tea leaves rich in enzyme activity
[0036] Tea solid culture medium: 50kg of summer and autumn green tea raw materials, add 15kg of water, mix well and sterilize at 110°C for 20min.
[0037] The activated Aspergillus niger and Aspergi...
Embodiment 2
[0043] (1) Source of bacteria
[0044] Aspergillus niger: purchased from China General Microorganism Culture Collection Management Center, strain preservation number: CGMCC3.316;
[0045] Aspergillus oryzae: purchased from China General Microorganism Culture Collection Management Center, strain preservation number: CGMCC3.863.
[0046] (2) Activation of bacteria
[0047] The species of Aspergillus niger and Aspergillus oryzae were inoculated on Chapeauer's agar medium for activation. Cha's agar medium: 30.0g sucrose, NaNO 3 3.0g, MgSO 4 ·7H 2 O7.5g, KCl0.5g, FeSO 4 4H 2 O0.01g, K 2 HPO 4 1.0g, 15.0g agar, 1L distilled water, pH6.0-6.5. Activation conditions: 24-48h activation culture at 28°C.
[0048] (3) Strain fermentation
[0049] Fermentation medium: corn stalk powder 68.0g, bran 40.0g, soybean meal powder 20.0g, (NH 4 ) 2 SO 4 6.0g, KH 2 PO 4 1.0g, FeSO 4 4H 2 O0.05g, MgSO 4 ·7H 2 O1.5g, distilled water 1L.
[0050] Inoculate the activated Aspergillus n...
Embodiment 3
[0057] With the method of embodiment 1, difference is:
[0058] (3) Preparation of semi-finished tea leaves rich in enzyme activity
[0059] Tea solid culture medium: 50kg of summer and autumn green tea raw materials, add 10kg of water, mix well and sterilize at 110°C for 20min.
[0060] The activated Aspergillus niger and Aspergillus oryzae were inoculated into the solid medium of tea leaves, and mixed and fermented at 35°C and RH60% for 10 days to obtain semi-finished tea products rich in cellulase, pectinase, tannase and protease activities.
[0061] (4) Tea extraction
[0062] The tea semi-finished raw material rich in enzyme activity is extracted at 45°C for 60 minutes according to the weight ratio of tea semi-finished product and water at 1:10, the extract is filtered to remove slag, and then passed through a ceramic membrane to obtain a tea extract, which is concentrated, After drying, instant tea powder is obtained.
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