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Cordyceps militaris culture medium capable of improving cordycepin content, and preparation method of cordyceps militaris culture medium

A culture medium and technology of Cordyceps militaris, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of rich nutrition, increased content, and convenient preparation

Inactive Publication Date: 2016-04-20
辽宁省农业科学院食用菌研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many disadvantages in the chemical synthesis of cordycepin. The main source of nucleosides such as cordycepin is the culture of Cordyceps militaris. Therefore, the optimization of the solid culture method of Cordyceps militaris has always been a hot spot in the research of Cordyceps militaris.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: A kind of production method of Cordyceps militaris that improves content of cordycepin

[0025] 1. Requirements for Cordyceps militaris cultivation area

[0026] A buffer zone greater than 30m must be set in the edible fungi cultivation area directly adjacent to the conventional farmland to avoid the influence of prohibited substances. The use of chemically synthesized pesticides is prohibited in and around the cultivation site. The water source should meet the requirements of GB5749.

[0027] 2. Cultivation substrate: The substrate of organic production or natural materials should be used.

[0028] 3. Production of mother species: inoculate the Cordyceps militaris strains in the PDA-enriched medium under sterile conditions, culture at a constant temperature of 23°C and avoid light, and culture them under light after the mycelium germinates, and select mycelia to grow neatly and robustly. , the color changes quickly after exposure to light, and when obs...

Embodiment 2

[0040] Embodiment 2: A kind of production method of Cordyceps militaris that improves content of cordycepin

[0041] 1. Requirements for Cordyceps militaris cultivation area

[0042] A buffer zone greater than 30m must be set in the edible fungi cultivation area directly adjacent to the conventional farmland to avoid the influence of prohibited substances. The use of chemically synthesized pesticides is prohibited in and around the cultivation site. The water source should meet the requirements of GB5749.

[0043] 2. Cultivation substrate: The substrate of organic production or natural materials should be used.

[0044] 3. Production of parent species: inoculate the Cordyceps militaris strains in the PDA-enriched medium under aseptic conditions, culture at a constant temperature of 23°C and avoid light, and culture them under light after the mycelia germinate. Select the mycelium that grows neatly and robustly , the color changes quickly after exposure to light, and when ob...

Embodiment 3

[0055] Embodiment 3 comparative example

[0056] 1) Production of mother seed: PDA medium: potato juice (heat 200g potato with 1000g water, cook, filter and extract the juice), glucose 20g, peptone 3g, MgSO 4 1.5g, KH 2 PO 4 3g, agar 15g, purified water 1000ml. The Cordyceps militaris strains were inoculated in PDA medium under aseptic conditions, cultured at a constant temperature of 23°C and protected from light, and cultured under light after the mycelia germinated. Select mycelia to grow neatly, strong, and change color quickly after exposure to light, and observe under a microscope, the strain with strong and erect mycelia is the original mother species.

[0057] 2) Production of liquid strains: Liquid culture medium: potato juice (heat 200g of potatoes and 1000g of water, cook and filter to obtain the juice), 20g of brown sugar, 3g of peptone, MgSO 4 1.5g, KH 2 PO 4 3g, purified water 1000ml. Take 1 piece of 8-10mm 2 The size of the original mother seed was inocu...

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Abstract

The invention relates to a cordyceps militaris culture medium capable of improving cordycepin content, and a preparation method of the cordyceps militaris culture medium. The method comprises the following steps: (1) preparing stock culture; (2) preparing liquid strain; (3) culturing sporocarp. The cordyceps militaris culture medium is prepared from the following main raw materials: potatoes, millet, barley, soybean meal, cane sugar, cocoon chrysalis powder, milk powder, brown sugar, peptone, purified water and the like. According to the preparation method, adenine is added into the formula of a liquid strain culture medium, and the adenine is coordinated and complementary with the soybean meal, the cane sugar, the cocoon chrysalis powder and other nutrient materials, so that the cordycepin content is effectively improved; the millet and the barley are taken as a sporocarp substrate; the cordycepin content of the cordyceps militaris sporocarp is 8.0-8.5mg / g. Furthermore, the cordyceps militaris culture is easy in obtaining of components, convenient to prepare, low in cost, simple in practical application and operation and easy to popularize, thus being suitable for large-scale production of cordyceps militaris.

Description

technical field [0001] The invention relates to the field of cultivation of medicinal edible fungi, in particular to a formula and a production method of a Cordyceps militaris culture medium for increasing the content of cordycepin. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris and Cordyceps militaris, belongs to the genus Cordyceps genus Ergotaceae of the fungus Ascomycetes, Hypocreae, and Cordyceps sinensis. , adenosine and other ingredients have nutritional and health effects such as enhancing immunity, regulating endocrine, protecting liver and lungs, enhancing sexual function, and various medicinal effects, and can be used as a substitute for Cordyceps sinensis. [0003] Cordycepin (Cordycepin) is an important secondary metabolite produced by Cordyceps militaris. As an important bioactive component of Cordyceps militaris, its chemical structure is 3′-deoxyadenosine. It was first discovered from Cordyceps militaris by Cunningham (19...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05G1/00C12R1/645
CPCC12N1/14C05C11/00C05F1/00C05F11/00
Inventor 李红刘娜宋莹田茂季肖千明张敏张季军肖军孙利平
Owner 辽宁省农业科学院食用菌研究所
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