Method for biodegrading residual polyacrylamide in coal slurry water
A polyacrylamide, biodegradation technology, applied in the directions of microorganism-based methods, methods of using microorganisms, biochemical equipment and methods, etc., can solve the problems of carcinogenicity, low operating costs, high costs, etc., and achieve obvious effects and low costs. , the effect of easy operation
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example 1
[0031] (1) Preparation of seeds: pick a ring from the Rhodopseudomonas test tube stored in the refrigerator and insert it into the enrichment medium. Cultivate in the culture medium for 36 hours at a temperature of 30°C, an initial pH of 6, and a stirring speed of 130 r / min to obtain degrading bacteria for acclimation;
[0032] (2) Domestication of degrading bacteria: get the seeds prepared in the above step 1 and insert them into the domestication medium containing 50ml enrichment medium and 20ml polyacrylamide slime water by 2% by volume, at a temperature of 30°C, Initial pH = 6, cultured for 72 hours at a stirring speed of 130r / min, poured out 20ml of the supernatant, then added 20ml of polyacrylamide slime water, and continued to cultivate for 5 days; take the first-stage domesticated bacterial solution by volume percentage 2% Insert into the acclimatization medium containing 150ml of polyacrylamide-containing slime water and 50ml of enrichment medium, cultivate for 72 hou...
example 2
[0037] (1) Preparation of seeds: pick a ring from the Rhodopseudomonas test tube stored in the refrigerator and insert it into the enrichment medium. Cultivate in the culture medium for 36 hours at a temperature of 30°C, an initial pH of 6, and a stirring speed of 130 r / min to obtain degrading bacteria for acclimation;
[0038] (2) Domestication of degrading bacteria: get the seeds prepared in the above step 1 and insert them into the domestication medium containing 50ml enrichment medium and 20ml polyacrylamide slime water by 2% by volume, at a temperature of 30°C, Initial pH = 6, cultured for 72 hours at a stirring speed of 130r / min, poured out 20ml of the supernatant, then added 20ml of polyacrylamide slime water, and continued to cultivate for 5 days; take the first-stage domesticated bacterial solution by volume percentage 2% Insert into the acclimatization medium containing 150ml of polyacrylamide-containing slime water and 50ml of enrichment medium, cultivate for 72 hou...
example 3
[0043] (1) Preparation of seeds: pick a ring from the Rhodopseudomonas test tube stored in the refrigerator and insert it into the enrichment medium. Cultivate in the culture medium for 36 hours at a temperature of 30°C, an initial pH of 6, and a stirring speed of 130 r / min to obtain degrading bacteria for acclimation;
[0044] (2) Domestication of degrading bacteria: get the seeds prepared in the above step 1 and insert them into the domestication medium containing 50ml enrichment medium and 20ml polyacrylamide slime water by 2% by volume, at a temperature of 30°C, Initial pH = 6, cultured for 72 hours at a stirring speed of 130r / min, poured out 20ml of the supernatant, then added 20ml of polyacrylamide slime water, and continued to cultivate for 5 days; take the first-stage domesticated bacterial solution by volume percentage 2% Insert into the acclimatization medium containing 150ml of polyacrylamide-containing slime water and 50ml of enrichment medium, cultivate for 72 hou...
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