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A kind of rapid detection method of orlistat

A detection method and orlistat technology, applied in the field of analytical chemistry detection, can solve the problems of high operating cost, expensive equipment, and high operator requirements, and achieve the effects of improved sensitivity, simple operation and obvious phenomenon.

Active Publication Date: 2016-11-30
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the above method can achieve the purpose of detection, the equipment is expensive, the operating cost is high, and the requirements for operators are also high, which is not conducive to widespread implementation in grassroots departments. People need a simple, fast, and low-cost detection method , in order to quickly identify whether orlistat is added to health products, food, and Chinese patent medicines

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Detection of tea

[0032] Reagent preparation: extraction liquid is petroleum ether (60-90℃); washing liquid is 70% (v / v) ethanol aqueous solution; hydroxylamine hydrochloride solution is 3.5wt% hydroxylamine hydrochloride-70% (v / v) ethanol-water solution; strong The alkaline solution is 1mol / L sodium hydroxide-70% (v / v) ethanol-water solution; the acidification solution is 1mol / L hydrochloric acid aqueous solution; the color developing solution is 9wt% iron trichloride aqueous solution.

[0033] Sample: Banshengyuan Tianxin Slimming Tea (the tea, the labelled dosage is 1 bag, the LC-MS / MS method determines that this product does not contain orlistat.)

[0034] The detection method, the steps are:

[0035] Take 1 bag of the above sample to be tested, grind the contents, take 1g of powder, add 2mL of petroleum ether (60-90℃), shake well for 3min, let stand for 4min, take 2mL of supernatant, then add 2mL of 70% (v / v) ethanol aqueous solution, shake gently for 2 minute...

Embodiment 2

[0037] Example 2: Detection of capsules

[0038] Reagent preparation: extraction liquid is n-hexane; washing liquid is 80% (v / v) ethanol solution; hydroxylamine hydrochloride solution is 4.0wt% hydroxylamine hydrochloride-80% (v / v) ethanol-water solution; strong alkali solution is 1.5mol / L sodium hydroxide-80% (v / v) ethanol-water solution; acidification solution is 1.5mol / L nitric acid solution; color developing solution is 12wt% ferric chloride solution.

[0039] Sample to be tested: Shuerjia Orlistat capsules (capsules, the indicated dosage is 1 capsule, and the LC-MS / MS method determines that this product contains orlistat.)

[0040] The detection method, the steps are:

[0041] Take 0.5g of the above sample powder to be tested, add 1mL of n-hexane, fully shake for 4min, let stand for 5min, take the supernatant, then add 1mL of 80% (v / v) ethanol solution, gently shake for 2min, and then Let the layers separate for 5 minutes, take the supernatant, and add 0.5 mL of 4.0wt% hydroxyl...

Embodiment 3

[0042] Result analysis: The color of the sample solution to be tested was wine-red, indicating that the sample contained orlistat, and the result was consistent with the actual situation. Example 3: Detection of tablets

[0043] Reagent preparation: the extraction solution is cyclohexane; the washing solution is 60% (v / v) methanol aqueous solution; the hydroxylamine hydrochloride solution is 3.0wt% hydroxylamine hydrochloride-60% (v / v) ethanol-water solution; the strong alkali solution is 0.5mol / L of sodium hydroxide-70% (v / v) ethanol-water solution; acidification solution is 0.5mol / L sulfuric acid solution; color developing solution is 6wt% ferric chloride solution.

[0044] Sample to be tested: Xiumeier slimming effervescent tablets (tablet, the indicated dosage is 1 tablet, and the LC-MS / MS method determines that this product does not contain orlistat.)

[0045] The detection method, the steps are:

[0046] Take 0.5g of the above sample powder to be tested, add 1mL of cyclohexane...

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Abstract

The present invention discloses an orlistat rapid detection method, a to-be-tested sample is extracted, treated with a washing solution, reacted with a hydroxylamine hydrochloride solution and a strong alkali solution for extraction, and then acidified for coloration, and whether orlistat is contained can be judged by color. The detection method is simple and low in cost for analysis, the to-be-tested sample is pretreated, and then reacted with a detection agent, so that the interference of relevant factors can be eliminated, observation of the color of the solution is facilitated, and whether the orlistat is contained in the sample can be judged more accurately. The detection method is strong in specificity, interfering substances can be eliminated by extraction, washing, extraction and separation, the sensitivity of the coloration reaction is increased, test result correct rate is high, the coloration reaction is rapid, the phenomenon is obviously, and the method is suitable for on-site rapid detection.

Description

Technical field [0001] The invention belongs to the field of analytical chemistry detection, and relates to a rapid detection method of orlistat. Background technique [0002] In recent years, with the improvement of people's living standards, obesity caused by factors such as unreasonable diet and lack of exercise has become increasingly serious. World Health Organization data show that in 2014, 39% of adults aged 18 and over were overweight and 13% were obese. Obesity can induce or aggravate diabetes, fatty liver, cardiovascular and cerebrovascular diseases, sleep apnea syndrome, musculoskeletal diseases, and certain cancers. It has become one of the ten major threats to human health. [0003] With people's urgent need for weight loss, various weight loss products are also increasing day by day. Chemical drugs for rapid weight loss usually bring obvious adverse reactions, while health products, foods and Chinese patent medicines are mostly derived from natural substances, so th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 咸瑞卿石峰巩丽萍梁翠荣王蕾蕾郭常川邢晟姜树银由鹏飞
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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