Strain of bacillus thuringiensis for production of antioxidant peptides and screening method thereof

A technology of Bacillus thuringiensis and Bacillus aureus, applied in the biological field, can solve the problems of preparing antioxidant peptides, achieve efficient decomposition and good application prospects

Inactive Publication Date: 2016-05-04
YANTAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been many related studies in recent years, so far there is no strain of ba

Method used

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  • Strain of bacillus thuringiensis for production of antioxidant peptides and screening method thereof
  • Strain of bacillus thuringiensis for production of antioxidant peptides and screening method thereof
  • Strain of bacillus thuringiensis for production of antioxidant peptides and screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The isolation and identification of embodiment 1 bacteria

[0027] 1. Isolation of bacteria and determination of enzyme activity

[0028] Put spoiled mackerel meat into sterile water at a ratio of 1:10 (g / v), mix well, and after gradient dilution, culture at 28°C for 24 hours, spread on beef extract peptone medium for preliminary separation, and use Pick the strains with different shapes, sizes, colors, and transparency on the plate with the inoculation loop to streak and purify the plate, and the isolated single colony is streaked on the solid casein medium, and the ratio of the diameter of the transparent circle of the single colony to the diameter of the colony Carry out further separation and purification. Select the strain with a larger ratio of the diameter of the transparent circle to the diameter of the colony for re-screening.

[0029] Using beef extract peptone medium, liquid casein medium without peptone, liquid casein medium without L-tyrosine, and liquid ...

Embodiment 2

[0033] The application example of this strain of embodiment 2

[0034] Dry and grind the mackerel meat, after sieving, add 50ml of pH6.8 phosphate buffer solution in a 250ml conical flask, the ratio of material to liquid is 2.9%, and inoculate HY-4 seed solution at 2%, at 28°C, 120r / min Under culture for 48h, the total antioxidant activity of the fermentation broth can reach 570.11U / g.

[0035]

[0036]

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Abstract

A strain of bacillus thuringiensis for production of antioxidant peptides is provided; the bacillus thuringiensis has the nucleotide sequence shown in SEQ ID No.1. A screening method comprises the steps: adding corrupted Spanish mackerel flesh into sterile water according to the ratio of 1:10 (g/V), mixing uniformly, carrying out gradient dilution, then culturing for 24 h under a condition of the temperature of 28 DEG C, coating a beef extract peptone culture medium, carrying out primary separation, and carrying out further separation purification according to the ratio values of the transparent circle diameters of single bacterial colonies to the diameter of a bacterial colony; selecting strains with the larger ratio values of the transparent circle diameters to the diameter of the bacterial colony, and rescreening; with the beef extract peptone culture medium, a liquid casein culture medium without addition of peptone, a liquid casein culture medium without addition of L-tyrosine and a liquid casein culture medium as culture media, determining the enzyme activity values of the strains with the larger ratio values of the transparent circle diameters to the diameter of the bacterial colony; screening out 5 strains of bacteria with stronger enzyme activity; and according to sequencing results, showing the nucleotide sequence in SEQIDNO.1.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bacillus thuringiensis strain producing antioxidant peptides and a screening method thereof. Background technique [0002] Since Harman proposed the free radical theory of aging in 1956, people have realized that free radicals in the human body are related to human aging and many diseases, so many antioxidants have been used in health food and medicine industries. In addition, antioxidants can delay the spoilage of fatty foods, and are also used in food preservation and storage. There are many types of antioxidants, and most of them are chemically synthesized antioxidants that have been widely used. Due to their toxicity and side effects, the governments of various countries strictly control their addition amount. At present, more researches are on the natural antioxidant active substances extracted from plants. In recent years, it has been found that peptides obtaine...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12R1/07
CPCC12N1/02C12N1/20C12N1/205C12R2001/075
Inventor 冯俊荣牟伟丽王爱敏康玲玲艾冰花
Owner YANTAI UNIV
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