Pathogen verticillium antagonistic bacterium and application thereof

A technology of Verticillium wilt bacteria and antagonistic bacteria is applied to cotton Verticillium wilt bacteria antagonistic bacteria. The application field of the antagonistic bacteria can solve the problems of no high-resistant cotton varieties, lack of specificity, environmental pollution and the like.

Inactive Publication Date: 2016-05-11
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of high-quality resistant source materials, disease-resistant breeding work is limited, and there is currently no high-resistant cotton variety against the disease
However, the commonly used chemical fungicides lack specificity, which will cause the production of drug-resistant strains and lead to environmental pollution.

Method used

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  • Pathogen verticillium antagonistic bacterium and application thereof
  • Pathogen verticillium antagonistic bacterium and application thereof
  • Pathogen verticillium antagonistic bacterium and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The isolation and screening method of Verticillium dahliae antagonistic bacteria comprises the following steps:

[0032] 1) After the cotton was harvested, 53 copies of cotton rhizosphere soil were taken from each cotton planting area in Henan as materials for the isolation of antagonistic bacteria;

[0033] 2) The improved LB medium was selected, and 53 soil materials were isolated by the dilution coating plate method, and a total of 537 strains of bacteria were isolated;

[0034] 3) The isolated strains were screened by the plate confrontation culture method, among which 14 strains had obvious antagonistic effect on the pathogen of Verticillium dahliae in cotton, and the strain numbered Y106 had the most obvious antagonistic effect, and the bacteriostatic rate was about 30%.

[0035] 1. Morphological observation results of antagonistic bacteria

[0036] The colony of Bacillus pumilus Y106 on the LB plate is opaque, milky white, round, viscous, moist and smooth; after...

Embodiment 2

[0054] Application of Verticillium dahliae antagonistic bacteria (CCTCCNO: M2014423) in the preparation of biocontrol agents for preventing and treating cotton Verticillium wilt. The specific steps are: taking Verticillium dahliae antagonistic bacteria for expanded culture, collecting the bacteria, and diluting with sterile water to obtain a liquid microbial agent. The methods for expanding the culture of strains and collecting thallines are all prior art, and will not be repeated here.

[0055] Application of Verticillium dahliae antagonistic bacteria (CCTCCNO: M2014423) in the control of cotton Verticillium wilt. Specifically, the cotton seeds are placed in the above-mentioned liquid bacterial agent, soaked, and then the seeds are taken out to dry. Bacteria agent concentration and seed soaking operation are prior art, no longer repeat them here.

Embodiment 3

[0057] Application of Verticillium dahliae antagonistic bacteria (CCTCCNO: M2014423) in the preparation of microbial organic fertilizers. The specific steps are: taking Verticillium dahliae antagonistic bacteria for expanded culture, collecting the bacteria, and diluting with sterile water to obtain a liquid microbial agent. Method is with embodiment 2.

[0058] Application of antagonistic bacteria against Verticillium dahliae (CCTCCNO:M2014423) in promoting the growth of cotton plants. Specifically: take the above-mentioned liquid bacterial agent and irrigate (or spray) the roots at the cotton seedling stage (or early flowering stage).

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Abstract

The invention discloses a pathogen verticillium antagonistic bacterium and application thereof, and belongs to the technical field of biological control over crop diseases. Bacillus pumilus Y106 is adopted as the pathogen verticillium antagonistic bacterium, the preservation number is CCTCC NO:M2014423, the preservation date is September 17, 2014, the preservation place is China Center for Type Culture Collection (CCTCC), and the preservation address is Wuhan University in Wuhan China. The bacterial strain is Gram-positive, mesophilic and aerobiotic and can resist the high temperature of 65 DEG C; the bacterial strain comprises the main morphological characteristics that the bacterial strain is shaped like a thin rod, the two ends are bluntly round, and the bacterial strain mostly exists in a single mode or in a chain-arranged mode. The pathogen verticillium antagonistic bacterium has the antagonistic effect on pathogen verticillium-verticillium dahliae, can inhibit germination of pathogen spores and can inhibit paroxysm of cotton verticillium wilt to a certain degree.

Description

technical field [0001] The invention relates to an antagonistic bacteria against Verticillium dahliae of cotton, and also relates to the application of the antagonistic bacteria, belonging to the technical field of biological control of crop diseases. Background technique [0002] Cotton is an important economic crop in my country, and it is grown in most provinces. Cotton Verticillium wilt (Verticillium wilt) is a soil-borne disease caused by Verticillium dahliae. It is distributed in various cotton producing areas in the world and is spreading day by day. The disease infects the vascular system of cotton, which is known as "the cancer of cotton", and can make fruits, beans, vegetables and other plants susceptible to diseases, resulting in a decrease in the quality and yield of agricultural products. How to effectively control the occurrence of cotton verticillium wilt, reduce its damage and ensure the stable and high yield of cotton has aroused widespread concern. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 安国勇王淼王刚程慧洁
Owner HENAN UNIVERSITY
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