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A fluorescence detection method for oxytetracycline based on graphene-based composite hydrogel

A composite hydrogel and graphene-based technology, applied in fluorescence/phosphorescence, measurement devices, and material analysis through optical means, can solve the problems of poor practicability, time-consuming, easy agglomeration, etc., and achieve a convenient and reliable detection process. The effect of controlling the detection range

Active Publication Date: 2018-08-21
DALIAN UNIV OF TECH
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  • Description
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  • Application Information

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Problems solved by technology

[0005] The present invention solves the shortcomings of the existing antibiotic detection methods such as complex preparation of nanomaterials, high energy consumption, instability and easy aggregation, time-consuming, poor practicability, etc., and provides a simple, fast, economical and practical method for detecting oxytetracycline in water method

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  • A fluorescence detection method for oxytetracycline based on graphene-based composite hydrogel
  • A fluorescence detection method for oxytetracycline based on graphene-based composite hydrogel
  • A fluorescence detection method for oxytetracycline based on graphene-based composite hydrogel

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Experimental program
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Effect test

Embodiment 1

[0030] Configure the determination of oxytetracycline content in the water sample:

[0031] (1) Preparation of graphene oxide:

[0032]Graphene oxide was prepared using a modified Hummers chemistry. The specific steps are as follows: Weigh 1.0g of graphite powder and slowly add it to 23mL of concentrated sulfuric acid. After fully stirring, slowly add 3g of KMnO 4 , slowly added while fully stirring, and then continuously ultrasonicated for 12 hours to obtain a dark brown solution, then slowly added 46mL deionized water, heated and boiled for 15min, then added 140mL high-purity water and 10mL hydrogen peroxide to terminate the reaction, and obtained bright yellow graphene oxide aqueous solution. After centrifugation, use 5% dilute hydrochloric acid 10000r / min centrifugal washing 2 times to remove impurities, and then use high-purity water 10000r / min centrifugal washing 5 times to remove impurities. After washing, the purified graphite oxide was taken out, put into a dialysi...

Embodiment 2

[0042] Configure the determination of oxytetracycline content in the water sample:

[0043] (1) Preparation of graphene oxide:

[0044] Graphene oxide was prepared using a modified Hummers chemistry. The specific steps are as follows: Weigh 1.0g of graphite powder and slowly add it to 23mL of concentrated sulfuric acid. After fully stirring, slowly add 3g of KMnO 4 , slowly added while fully stirring, and then continuously ultrasonicated for 12 hours to obtain a dark brown solution, then slowly added 46mL deionized water, heated and boiled for 15min, then added 140mL high-purity water and 10mL hydrogen peroxide to terminate the reaction, and obtained bright yellow graphene oxide aqueous solution. After centrifugation, use 5% dilute hydrochloric acid 10000r / min centrifugal washing 2 times to remove impurities, and then use high-purity water 10000r / min centrifugal washing 5 times to remove impurities. After washing, the purified graphite oxide was taken out, put into a dialys...

Embodiment 3

[0054] Configure the determination of oxytetracycline content in the water sample:

[0055] (1) Preparation of graphene oxide:

[0056] Graphene oxide was prepared using a modified Hummers chemistry. The specific steps are as follows: Weigh 1.0g of graphite powder and slowly add it to 23mL of concentrated sulfuric acid. After fully stirring, slowly add 3g of KMnO 4 , slowly added while fully stirring, and then continuously ultrasonicated for 12 hours to obtain a dark brown solution, then slowly added 46mL deionized water, heated and boiled for 15min, then added 140mL high-purity water and 10mL hydrogen peroxide to terminate the reaction, and obtained bright yellow graphene oxide aqueous solution. After centrifugation, use 5% dilute hydrochloric acid 10000r / min centrifugal washing 2 times to remove impurities, and then use high-purity water 10000r / min centrifugal washing 5 times to remove impurities. After washing, the purified graphite oxide was taken out, put into a dialys...

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Abstract

The invention belongs to the technical field of environmental monitoring, and relates to an oxytetracycline fluorescence detection method for antibiotics in water based on graphene-based compound hydrogel. Graphene dispersed liquid is adopted as a monomer, adenosine and an aptamer are adopted as a cross-linking agent together, graphene oxide lamellas originally dispersed in a solution are connected together, and a three-dimensional macrostructure is formed. Oxytetracycline aqueous solutions with different concentrations are added to a hydrogel system for a simple soaking process, supernate is taken for fluorescence intensity testing, and quantitative fluorescence detection of the concentration of oxytetracycline can be achieved. In the prepared hydrogel system with different proportions, the detection range of oxytetracycline can be controllably adjusted, and the method can be applied to different types of actual water for detection. Compared with a traditional nano material sensing detecting method, the method is fast in nano material preparation, simple, convenient to use, mild in preparation condition and high in environmental stability and practicality of the hydrogel material.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and relates to a graphene-based composite hydrogel-based oxytetracycline fluorescence sensing and detection method. Background technique [0002] Tetracycline is a class of broad-spectrum antibiotics produced by actinomycetes, including naturally occurring tetracycline (tetracycline, TET), oxytetracycline (oxytetracycline, OTC), doxycycline (Doxycycline), metacycline (methacycline) etc., all of which contain naphthacene basic skeletons, and are widely used in various bacteria and Rickettsia, Chlamydia, and Mycoplasma infections. However, the abuse of antibiotics, including tetracyclines, will cause antibiotics to accumulate in environmental media (Environmental Science & Technology, 2015, 49 (11), 6772-6782.), and then enrich in the human body through food chains (Environmental Science & Technology, 2015, 49 (8), 5070-5079.). In addition, the abuse of antibiotics can also induc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N2021/6491
Inventor 赵慧敏谭冰甘小荣全燮
Owner DALIAN UNIV OF TECH
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