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A kind of high-efficiency induction method of Polygonatum chinensis in vitro rhizomes

A rhizome and in vitro technology, which is applied in the field of high-efficiency induction of Polygonatum rhizome in vitro, can solve problems such as cumbersome and complicated production process of Polygonatum tissue culture, unsuitability for large-scale production, and contradictory market demand, and achieve the application value of large-scale production and the survival rate High, strong proliferative effect

Active Publication Date: 2017-09-15
遵义市龙驰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the above-mentioned technical problems of rhizome high-efficiency induction method, the present invention has adopted a kind of Polygonatum in vitro high-efficiency induction method, has solved the cumbersome and complicated procedure of Polygonatum tissue culture production process in the prior art, is not suitable for the problem of large-scale production, both guaranteed uncertain The induction rate of buds also promotes the efficient induction of isolated rhizomes, and finally solves the problem of contradiction between the protection of Polygonatum resources and market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take the rhizomes with buds and wash them, scrub the surface with 75% ethanol, rinse with tap water, soak in 75% ethanol for 30s, rinse with sterilized deionized water twice, sterilize with 2.5% sodium hypochlorite for 5 minutes, rinse with sterilized deionized water for 2 Once again, sterilized with 2.5% sodium hypochlorite for 5 min, rinsed with deionized water for 5 times, blotted the surface moisture of the material with sterile filter paper, cut the material into bud tubers with a diameter of 0.5 cm and placed them on the adventitious bud induction medium (MS+ 6-BA 1.0mg / L+2,4-D 0.1mg / L+commercial white sugar 3%+agar powder 5g / L, pH 5.8~6.0), 25~27℃, light 1600Lx, light 12h / d. After 35 days, 90% of the explants had differentiated adventitious buds; the tubers with adventitious buds differentiated were cut into 0.5 cm in diameter and placed in adventitious bud growth medium (MS+6-BA0.5mg / L+2,4-D 0.1mg / L+ commercially available white sugar 3%+ agar powder 5g / L, pH 5....

Embodiment 2

[0027] Take the rhizomes with buds and wash them, scrub the surface with 75% ethanol, rinse with tap water, soak in 75% ethanol for 30s, rinse with sterilized deionized water twice, sterilize with 2.5% sodium hypochlorite for 5 minutes, rinse with sterilized deionized water for 2 Once again, sterilized with 2.5% sodium hypochlorite for 5 min, rinsed with deionized water for 5 times, blotted the surface moisture of the material with sterile filter paper, cut the material into bud tubers with a diameter of 0.5 cm and placed them on the adventitious bud induction medium (MS+ 6-BA 1.5mg / L+2,4-D 0.2mg / L+commercially available white sugar 3%+agar powder 5g / L, pH 5.8~6.0), 25~27℃, light 1600Lx, light 12h / d. After 35 days, 90% of the explants had differentiated adventitious buds; the tubers with differentiated adventitious buds were cut into 0.5 cm in diameter and placed in adventitious bud growth medium (MS+6-BA1.0mg / L+2,4-D 0.2mg / L + commercially available white sugar 3% + agar powd...

Embodiment 3

[0029] Take the rhizomes with buds and wash them, scrub the surface with 75% ethanol, rinse with tap water, soak in 75% ethanol for 30s, rinse with sterilized deionized water twice, sterilize with 2.5% sodium hypochlorite for 5 minutes, rinse with sterilized deionized water for 2 Once again, sterilized with 2.5% sodium hypochlorite for 5 min, rinsed with deionized water for 5 times, blotted the surface moisture of the material with sterile filter paper, cut the material into bud tubers with a diameter of 0.5 cm and placed them on the adventitious bud induction medium (MS+ 6-BA 2.0mg / L+2,4-D 0.5mg / L+commercially available white sugar 3%+agar powder 5g / L, pH 5.8~6.0), 25~27℃, light 1600Lx, light 12h / d. After 35 days, 90% of the explants differentiated into adventitious buds; the tubers with adventitious buds were cut into 0.5 cm in diameter and placed in adventitious bud growth medium (MS+6-BA1.5mg / L+2,4-D 0.2mg / L + commercially available white sugar 3% + agar powder 5g / L, pH 5....

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PUM

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Abstract

The invention provides an efficient inducing method of in-vitro rhizomes of Polygonatum sibiricum Red. The method mainly comprises steps as follows: (1) rhizomes with buds, tender leaves or petiole of Polygonatum sibiricum Red are selected and serve as explants; (2) induced culture of adventitious buds is performed; (3) proliferation culture of the adventitious buds is performed; (4) induced culture of the in-vitro rhizomes is performed; (5) the in-vitro rhizomes are directly transplanted or stored. The in-vitro rhizomes are directly induced in a bottle, a process is simple, the induction rate is high, the proliferation capability is high, the survival rate of the transplanted in-vitro rhizomes is high, the in-vitro rhizomes can be directly transplanted to a large field or stored without rooting culture or special domestication, and a large quantity of high-quality Polygonatum sibiricum Red seedlings can be produced in short time.

Description

technical field [0001] The invention relates to a method for tissue culture of Chinese medicinal materials, in particular to a method for efficiently inducing isolated rhizomes of Rhizoma Polygonatum. Background technique [0002] Polygonatum sibiricum is the rhizome of Polygonatum sibiricum Red, P. kingianum Coll.eHtemsl. or P. cyrtonema Hua, a perennial herb of the family Liliaceae. The role of essence. Modern studies have shown that Polygonatum contains niacin, aldehydes, liquid temperament, starch, diaminobutyric acid, Polygonatum polysaccharides and oligosaccharides, etc., which have the effects of anesthesia and lowering animal blood pressure, and have a positive effect on hyperglycemia caused by adrenaline. Inhibitory effect, to prevent atherosclerosis and hepatic fat infiltration, also has a good effect on improving kidney function. Polygonatum is also a good antibacterial medicine blindly, and it has a certain inhibitory effect on typhoid bacillus, tubercle bacill...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 刘红美梁大勇方小波石建龙
Owner 遵义市龙驰生物科技有限公司