Culture method for thesium chinense tissue culture vessel seedlings

A technology of thymegrass and tissue culture bottle, which is applied in the biological field, can solve the problems of low transplanting survival rate and low yield of thymegrass, solve the shortage of thymegrass resources, avoid rooting culture, and transplant and production cycle short effect

Inactive Publication Date: 2016-05-18
GUIZHOU EDUCATION UNIV
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AI Technical Summary

Problems solved by technology

[0004] But there is still the problem of low survival rate of transplanting, and both artificially cultivated tissue cultured seedlings and seedlings need host plant p

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0021] A kind of seedling raising method of thyme tissue culture bottle seedling, comprises the following steps:

[0022] (1) Obtaining explants

[0023] Rinse the young thyme twigs under tap water, then sterilize the surface with 75% alcohol for 30-40s, then sterilize in 0.1% mercuric solution for 6-7min, rinse with sterile water for 3-5 times, and finally in Under sterile conditions, cut the leaves into small sections with wounds around 1 cm long;

[0024] (2) Callus induction and subculture

[0025] The cut leaves were inoculated on the callus induction medium, and cultured and induced at a temperature of 24±1°C and a light culture condition of 1500-2000Lx, the periphery of the leaves swelled rapidly, and the callus was induced, and the induction rate was It can reach 100%, and most of the induced callus is light yellow or light green callus with loose texture. After 3 weeks, the callus with stable morphological characteristics and growth rate will be induced to be subcu...

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Abstract

The invention discloses a culture method for thesium chinense tissue culture vessel seedlings. The culture method includes the steps that tender and strong branch blades of wild thesium chinense plants in spring are selected as explants, a callus tissue induction medium is inoculated with the explants to generate callus tissue through culture and induction, and after 3 weeks, the callus tissue generated through induction is transferred to a fresh culture medium to be subcultured; the callus tissue obtained through subculturing is proliferated in a light culture differentiation mode to the 30th day, and an MS solid culture medium is used for induction culture for 15-20 days to obtain the thesium chinense root-free seedlings; the induced root-free seedlings are cut into single seedlings, a proliferation culture medium is inoculated with the single seedlings, and the proliferation coefficient is 8.5 by means of 26-day culture. The thesium chinense root-free seedlings obtained through rapid proliferation are thin and need strong seedling culture, after strong seedling culture is conducted for 15 days, blades are thickened and widened, stems are thickened, the seedlings are strong and normal in color, the culture medium attached to the stem base is washed away, and the seedlings are spread and cooled for 3 days to serve as kaempferol extract raw materials. The culture method has the advantages of being high in proliferation speed, short in production cycle and capable of realizing large-scale production, being not limited by seasons, achieving continuous production and not occupying cultivated land.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a seedling raising method of Thyme chinensis tissue culture bottle seedlings. Background technique [0002] thyme ( Thesium Chinense Turcz ) is a plant of Sandalaceae, the whole herb is used as medicine, it has the functions of clearing away heat and detoxifying, tonifying kidney and astringent essence, and can treat acute mastitis, pneumonia, pneumonic intestine, tonsillitis, upper respiratory tract infection, kidney deficiency and low back pain, dizziness, nocturnal emission, and slippery essence. Spectrum antibacterial herbal medicine. The content of thymosin in thyme grass is relatively high, and it is easy to separate and extract, so it is a better raw material for the production of thymelin. Thyme grass is a semi-parasitic plant, which cannot survive independently without the host plant. In recent years, with the gradual decrease of the host plant, the yield of ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄燕芬何军王自布刘向阳曹永高
Owner GUIZHOU EDUCATION UNIV
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