A rapid method for constructing a chicken piebald kidney model

A construction method and a variegated kidney technology are applied in the field of rapid construction of a chicken variegated kidney model, which can solve the problems of insufficiently obvious clinical symptoms of the model and complicated steps, and achieve the effects of simple method, obvious clinical symptoms, and typical necropsy characteristics.

Active Publication Date: 2019-03-22
POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

The clinical symptoms of the model constructed by the above method are not obvious enough, and it needs to be investigated through indicators such as chick weight gain rate, feed intake, water intake, mortality, serum urate content, kidney pathological changes, etc. The steps are cumbersome and need to be further improved

Method used

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  • A rapid method for constructing a chicken piebald kidney model
  • A rapid method for constructing a chicken piebald kidney model
  • A rapid method for constructing a chicken piebald kidney model

Examples

Experimental program
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Embodiment 1

[0012] The rapid construction of embodiment 1 chicken piebald kidney model

[0013] 1. Experimental materials and methods

[0014] 1.1 Materials: ammonium chloride, produced by Tianjin Fuchen Chemical Reagent Factory, batch number 20071127, polyethylene glycol-400, produced by Bazhou Chemical Branch Factory of Tianjin Quartz Clock Factory, batch number, 20010306; compound feed AC820R in the early stage of laying hen breeding, Jinan Anchi Produced by Feed Co., Ltd. on January 15, 2015, crude protein ≥ 15%; 100-day-old SPF cock, provided by Shandong Haotai Experimental Animal Center; SPF chicken stainless steel isolator: Suzhou Fengshi Experimental Animal Equipment Co., Ltd.

[0015] 1.2 Method: The experiment was divided into 5 groups, which were experimental groups 1 to 4 and a blank control group, with 10 chickens in each group. See Table 1 for details of drug addition, addition amount, addition route, and addition time.

[0016] Table 1 Experimental design table

[0017] ...

Embodiment 2

[0025] Example 2 Rapid construction of SPF chicken piebald kidney model

[0026] 1. Experimental materials and methods

[0027] 1.1 Materials: ammonium chloride, produced by Tianjin Fuchen Chemical Reagent Factory, batch number 20071127, polyethylene glycol-400, produced by Bazhou Chemical Branch Factory of Tianjin Quartz Clock Factory, batch number, 20010306; compound feed AC820R in the early stage of laying hen breeding, Jinan Anchi Produced by Feed Co., Ltd. on January 15, 2015, crude protein ≥ 15%; 100-day-old SPF chicken, provided by Shandong Haotai Experimental Animal Center; SPF chicken stainless steel isolator: Suzhou Fengshi Experimental Animal Equipment Co., Ltd.

[0028] 1.2 Method: The experiment was divided into drug group and blank control group, with 15 chickens in each group. The drinking water of the test group was supplemented with 1% ammonium chloride and 2% polyethylene glycol-400 for 15 consecutive days. The blank control group had normal food intake and ...

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Abstract

The invention discloses a rapid construction method of a chicken mottled kidney model. According to the method, 1% ammonium chloride and 2% polyethylene glycol-400 are added to drinking water for 50 to 120-day-old SPF (specific pathogen free) chicken and added continuously for 10-15 days, and the chicken freely drink water and forage; the chicken mottled kidney model is determined to be successfully constructed if the model chicken have the symptoms as follows: cockscombs and wattle of the model chicken suffer from cyanosis, and claws of the chicken are dried up and dehydrated; the kidney swells and is mottled on the basis of necropsy, and a large amount of grey white urate deposit in ureters. The model constructed with the method has remarkable clinical symptoms and typical necropsy characteristics and is suitable for research of a medicine for preventing and treating the chicken mottled kidney.

Description

technical field [0001] The invention relates to a method for constructing a chicken disease model, in particular to a method for rapidly constructing a chicken mottled kidney model. Background technique [0002] In recent years, due to the excessive pursuit of growth rate and feed-to-meat ratio in the meat and poultry industry, high-protein and high-energy feeds are generally used, and broiler metabolism produces a large amount of uric acid, which increases the burden on the kidneys. If raw materials such as poor-quality cake, meat and bone meal, and moldy corn are used in the feed, and the stocking density is high (35kg-45kg / m 2 ), poor ventilation, renal-type infectious bronchitis virus infection, long-term heavy use of chemical antibiotics, etc., can easily cause kidney damage, block the excretion of a large amount of uric acid, accumulate in the kidney to form urate, cause kidney swelling and bleeding, mottled , Severe cases can cause the occurrence of gout. According ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
CPCA61K49/0008
Inventor 黄中利林树乾宋敏训冯敏燕傅剑赵增成李桂明
Owner POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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