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A method for rapidly constructing chicken adenomyosis gastritis model

A gastroenteritis model technology, applied in the field of animal husbandry, etc., can solve the problems of unstable model construction, chicken adenomyosis model restricting pathogenesis research, etc., and achieve the effect of simple method, obvious clinical symptoms, and typical autopsy characteristics

Active Publication Date: 2022-04-19
POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of the etiology, there is no good method for model construction. Although there is a method for constructing adenomyosis gastritis with mycotoxins, we found that the model construction is unstable after conducting experiments
How to quickly construct a chicken adenomyosis model has become a bottleneck that seriously restricts its pathogenesis research and drug development

Method used

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  • A method for rapidly constructing chicken adenomyosis gastritis model
  • A method for rapidly constructing chicken adenomyosis gastritis model
  • A method for rapidly constructing chicken adenomyosis gastritis model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the rapid construction of SPF chicken adenomyosis gastritis model (rhubarb-acetic acid)

[0024] 1. Experimental materials and methods

[0025] 1.1 Materials: Rhubarb, purchased from Jinan Jianlian Traditional Chinese Medicine Store, made into decoction with crude drug ratio of 1:1, for use; glacial acetic acid, Tianjin Fuyu Fine Chemical Co., Ltd.; Duck IL-2ELISA KiT, Duck IL-4ELISA KiT , Duck TNF-αELISA KiT, product of Wuhan Genome Biotechnology Co., Ltd., α-amylase, product of Nanjing Jiancheng Bioengineering Research Institute. The compound feed AC820R in the early stage of laying hens was produced by Jinan Anchi Feed Co., Ltd. on July 09, 2020, with crude protein ≥ 15%; 50-day-old SPF roosters were provided by Shandong Haotai Experimental Animal Center; SPF chicken stainless steel isolator: Suzhou Feng Produced by Shi Experimental Animal Equipment Co., Ltd. Glass thermometer, produced by Shenzhen Junshi Medical Instrument Co., Ltd.

[0026] 1.2 Met...

Embodiment 2

[0055] Embodiment 2: Construction of hybrid broiler glandular gizzard model (rhubarb-acetic acid)

[0056] 1. Experimental materials and methods

[0057] 1.1 Materials: rhubarb, purchased from Jinan Jianlian Traditional Chinese Medicine Store, made into a water decoction with a crude drug ratio of 1:1, for use; glacial acetic acid, Tianjin Fuyu Fine Chemical Co., Ltd.; laying hen pre-breeding compound feed AC820R, Jinan Anchi feed Co., Ltd. produced on July 09, 2020, the implementation of the standard GB / T5916-2008, crude protein ≥ 15%; 20-day-old hybrid broiler, Liaocheng Aoxiang Poultry Industry Co., Ltd.; glass thermometer, produced by Shenzhen Junshi Medical Equipment Co., Ltd. .

[0058] 1.2 Method

[0059] 1.2.1 Grouping: The experiment was divided into experimental group 1, experimental group 2 and blank control group, with 15 chickens in each group. Weigh the numbers separately.

[0060] 1.2.2. Medication method: add 2.5g / kg.w rhubarb water decoction and 1.0% aceti...

Embodiment 3

[0074] Embodiment 3: the rapid construction of SPF chicken adenomyosis gastritis model (rhubarb-polyethylene glycol)

[0075] 1. Experimental materials and methods

[0076] 1.1 Materials: Rhubarb, purchased from Jinan Jianlian Traditional Chinese Medicine Store, made into a water decoction with a crude drug ratio of 1:1, for use; polyethylene glycol-400, produced by Bazhou Chemical Branch of Tianjin Quartz Clock Factory, batch number, 20010306; Duck IL -2 ELISA KiT, Duck IL-4ELISA KiT, Duck TNF-αELISA KiT, products of Wuhan Genome Biotechnology Co., Ltd., α-amylase, products of Nanjing Jiancheng Bioengineering Research Institute; compound feed AC820R for laying hens, Jinan Anchi feed Co., Ltd. produced on July 09, 2020, crude protein ≥ 15%; 50-day-old SPF cock, provided by Shandong Haotai Experimental Animal Center; SPF chicken stainless steel isolator: Suzhou Fengshi Experimental Animal Equipment Co., Ltd.; glass thermometer, Produced by Shenzhen Junshi Medical Devices Co., ...

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Abstract

The invention discloses a method for rapidly constructing a chicken adenomyosis gastritis model. The method can directly add rhubarb and acetic acid or polyethylene glycol-400 to chicken drinking water to construct a chicken adenomyosis gastritis model. The model constructed by this method has obvious clinical symptoms and typical autopsy characteristics, and is suitable for the research of drugs for the prevention and treatment of chicken adenomyosis. This method also provides a unique method and idea for the construction of chicken adenomyosis gastritis model.

Description

technical field [0001] The invention relates to a method for rapidly constructing a chicken adenomyosis gastritis model, and belongs to the technical field of animal disease model construction. Background technique [0002] Adenoid gastritis is a disease characterized by enlargement of the glandular stomach and erosion and ulceration of the gizzard. It can occur in different breeds of laying hens, broilers and native chickens, and is more common in young chickens. The causes of the disease include viruses, bacteria, improper feeding and management, moldy feed, refueling stress, and water quality problems. The treatment methods include antiviral, mold control and traditional Chinese medicine treatment. At present, the disease is on the rise in the broiler farming industry, and has become one of the most common diseases that endanger the production of poultry farming, bringing serious economic losses to many farmers. The veterinary syndrome of this disease ranges from spleen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027
CPCA01K67/027A01K2207/25A01K2227/30A01K2267/0337
Inventor 黄中利林树乾赵增成刘月月殷斌杨世发李桂明衣云鹏李颖李真
Owner POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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