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Preparation method of bacterial cellulose thin film with usage of surfactant

A bacterial cellulose and surfactant technology, applied in the field of bacterial cellulose film preparation, can solve the problems of increased time and cost, uneven appearance of the membrane, and thin membranes are too soft and easy to break, so as to reduce time and cost Effect

Active Publication Date: 2016-05-18
FOOD IND RES & DEV INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The bacterial cellulose produced by the fermentation of Gluconacetoacetobacter xylinum can increase the thickness of the membrane by increasing the number of days of culture, but the thin membrane produced by too short culture time is too soft and easy to break, and the appearance of the membrane is prone to unevenness the problem
Therefore, post-processing methods such as extrusion or cutting are often used to obtain thin films, which increases the time and cost required for the process

Method used

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Examples

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Effect test

preparation example Construction

[0022] The preparation method of the present invention is to add a surfactant with low bacteriostasis to the medium to promote the uniform dispersion of the bacteria in the medium, and the film thickness can be recovered after standing for 1.5-3 days. <1mm, non-breakable and transparent bacterial cellulose film. In the method of the present invention, the culture medium added with the surfactant can be placed in a container with a general opening, and the container is covered for static culture. The transparent bacterial cellulose film is formed on the surface of air and liquid culture medium, so the film can be conveniently collected from the container, washed with water or washed with alkaline aqueous solution and then washed with water to make the pH of the film approximately The thickness can be prepared for 7 <1mm transparent bacterial cellulose film product.

[0023] Surfactants suitable for use in the present invention include, but are not limited to, anionic surfactants, ...

Embodiment 1

[0025] The Gluconacetobacterxylinus colony was inoculated into a 250 mL conical flask containing 100mLAC medium (see Table 2 below). After 3 days of cultivation at 30°C and 100 rpm, the bacterial solution was taken out and the amount of inoculation was 10% by volume (v / v) Inoculate 100 mL of liquid media in a nylon box (area 20*20*8.5cm), and then close the lid for box culture. The liquid medium (YE medium) contains the components listed in Table 1 below, and is further added with 1% Tween80 nonionic surfactant, and the% is based on the volume of the liquid medium. The box was placed flat and placed at 30°C for 2 days to obtain a transparent bacterial cellulose film. Open the lid, take out the film from the box, then wash with reverse osmosis (RO) water at 80°C for 10 minutes, and then wash at room temperature until the pH of the film is 7, so that light with a thickness of 0.8mm and a wavelength of 550nm is obtained A transparent bacterial cellulose film with a penetration of...

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Abstract

The invention relates to a preparation method of a bacterial cellulose thin film which is about 90% in transmittance to light being 550 nm in wavelength. The preparation method includes the step of in a container filled with a culture solution on which bacteria has been inoculated, performing static cultivation to the bacteria in the container which is covered by a cover for a certain time until a piece of bacterial cellulose thin film is formed, wherein the culture solution includes 1000-20000 ppm of a surfactant. The thin film can be used in skin dressing, packaging materials and industrial materials and the like.

Description

Technical field [0001] The present invention relates to a method for preparing a bacterial cellulose film, and in particular to a method for preparing a bacterial cellulose film having a penetration of about 90% to light with a wavelength of 550 nm. Background technique [0002] The bacterial cellulose produced by the fermentation of Acetobacter xylonum can increase the thickness of the membrane with the increase of the cultivation days, but the thin membrane produced by the too short cultivation time is too soft and easy to break, and the appearance of the membrane is easy to appear uneven The problem. Therefore, post-processing methods such as extrusion or cutting are often used to obtain thin films, which increases the time and cost required for the manufacturing process. [0003] Japanese Patent Publication No. JP2009296979A discloses that this invention utilizes the addition of a non-ionic surfactant with a concentration range of 0.05-300 ppm to increase the yield (dry film w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02C12R1/38
Inventor 郭秋媚傅威昌赖进此廖启成
Owner FOOD IND RES & DEV INST