Nanobubble-containing composition and use thereof

A composition and micro-bubble technology, which can be applied to gas-containing food ingredients, medical preparations containing active ingredients, and drug combinations, etc., can solve the problems of reduced survival rate, poor flavor, leakage of contents, etc. effect on cell proliferation

Inactive Publication Date: 2016-05-18
AQUA ZEST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, when the cells are frozen, there are problems such as a decrease in the survival rate, damage to the cells, leakage of the contents, deterioration of the taste, and deterioration of the flavor.

Method used

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  • Nanobubble-containing composition and use thereof
  • Nanobubble-containing composition and use thereof
  • Nanobubble-containing composition and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] iPS cell culture medium was prepared in the following manner. 12.5 g of Glasgow's minimal essential medium (Sigma, G6148) and 2.75 g of sodium bicarbonate (Sigma, S5761) were added to 1 L of distilled water (DW) or the composition of the present invention (APAS), and stirred. To this solution, the reagents were added in such a way that the following final concentrations were obtained: 0.1 mM MEM non-essential amino acid solution (Gibco, 11140) with 1 mM sodium pyruvate solution (Sigma, S8636), 1% fetal calf serum ( CCB, 171012), 1% penicillin streptomycin (Gibco, 15140), 5.4% Knockout serum substitute (Gibco, 10828), 0.1mM 2-mercaptoethanol (Wako, 137-06862), 2000U / ml of Leukemia inhibitory factor (Millipore, ESG1107). After stirring until homogeneous and dissolved, filter-sterilize it with a 0.45 μm filter and use it as a cell maintenance medium.

[0079] The result of cultivating figure 1 shown. As a result, in the case of using the composition of the present inve...

Embodiment 2

[0080] (Example 2) Study on Antioxidative Effect Using Mouse Vascular Endothelial Cell Line (MAEC)

[0081]Water containing the composition of the present invention, nano-bubble water (nano-bubble water containing air), hydrogen nano-bubble water (nano-bubble water containing more than 70% of the total of dissolved gas, microbubbles, and nanobubbles) are used. M199 medium (SIGMA) was prepared, and 10% fetal bovine serum, 100 IU / ml penicillin and 100 μl / ml streptomycin were added as a culture medium. After culturing MAEC for 24 hours in a medium using various waters, the culture medium was replaced with a fresh one, and further cultured for 2 hours. Then, 30 μg / mL of antimycin was added to each medium, and cultured for 30 minutes. As a control, ethanol used as a solvent was added in an amount equivalent to that contained in the antimycin solution at 0.06 v / v%. It is known that antimycin has the effect of producing ROS (reactive oxygen species, Reactive Oxygen Species) in cell...

Embodiment 3

[0083] (Example 3) Study of ATP production during oxidative stress load in mouse vascular endothelial cell line (MAEC)

[0084] Next, in order to confirm the effect of ATP-producing activated water on the mitochondrial function that is decreased under oxidative stress load in MAEC, intracellular ATP production was studied. As in the above experiment, MAEC cells were cultured in a culture medium prepared with water containing the composition of the present invention, nanobubble water, and hydrogen nanobubble water. After culturing MAEC for 24 hours in a medium using various waters, the medium was replaced with a fresh medium using various waters, and further cultured for 2 hours. Next, 50 μg / ml antimycin, 0.06 v / v% ethanol, and 500 μmol / L hydrogen peroxide were added to each medium. In addition, as a control for the antioxidation effect, N-acetylcysteine ​​(NAC), an antioxidant that acts on conventional reactive oxygen species, was used. The medium for the control of the anti...

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Abstract

[Problem] The present invention provides a composition containing nanobubbles containing hydrogen, oxygen, and nitrogen, and the use thereof. [Solution] A composition containing, as an active ingredient, nanobubbles having a diameter of 30 Mum or less and containing 0.45-0.55 ppm of hydrogen, 10-12.5 ppm of oxygen, and 7-8 ppm of nitrogen. A mitochondria-activating composition, cell growth promoting agent, cell preservation liquid or cryopreservation liquid containing this composition, and a method for using this composition, agent, or liquid. A food or beverage, or a food or beverage raw material, produced using this composition.

Description

technical field [0001] The invention relates to a composition containing nanobubbles and its application. Specifically, it relates to a composition containing nanobubbles and activating mitochondria and its use. Background technique [0002] As a method for promoting growth of cultured cells by dissolving gas in a liquid using microbubble and nanobubble technology, for example, a method such as Patent Document 1 is disclosed. However, in Patent Document 1, there is no specific disclosure of what size and composition of microbubbles and nanobubbles can be used to promote the proliferation of what kind of cells, to what extent, etc., so as to seek to develop more practical compositions, methods, device. In addition, effective methods for promoting growth have been further sought. [0003] Hydrogen water, hydrogen oxygen water, and the like are known as products in which gas is mixed into drinking water using microbubbles and nanobubbles (for example, Patent Document 2). Ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07C12N5/04A01N1/02A23L33/16A61K9/08A61K33/00A61P3/10A61P9/10A61P43/00
CPCA01N1/0221A23L2/54A23L33/16A23P30/40A23V2002/00A61K9/0019A61K9/0095A61K9/14A61K33/00A61P3/10A61P9/10A61P39/06A61P43/00A61K2300/00A23V2200/25A23V2250/10
Inventor 亀井一郎
Owner AQUA ZEST
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