Temperature-sensitive recombinant corynebacterium glutamicum producing glutamic acid and application thereof

A technology of glutamic acid sticks and glutamic acid production, which is applied in the field of genetic engineering and fermentation engineering, can solve the problems of low acid production rate, low conversion rate of sugar and acid, vitality of aging strains, etc., and achieve the release of feedback repression and Inhibition, enhanced cell wall permeability, enhanced translocation effects

Active Publication Date: 2016-05-25
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

The wild strains of glutamic acid-producing bacteria isolated from nature often cannot meet the requirements of fermentation production development in terms of production performance
Glutamic acid production strains have a large natural variation ability, and the negative variation frequency is usually higher than the positive variation frequency, resulting in the

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  • Temperature-sensitive recombinant corynebacterium glutamicum producing glutamic acid and application thereof
  • Temperature-sensitive recombinant corynebacterium glutamicum producing glutamic acid and application thereof
  • Temperature-sensitive recombinant corynebacterium glutamicum producing glutamic acid and application thereof

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Embodiment Construction

[0019] The present invention is further described below in conjunction with specific embodiments, and the advantages and characteristics of the present invention will become clearer along with the description. However, these examples are only exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention. The methods, ingredients and dosages involved in the following examples are conventional methods known to those skilled in the art unless otherwise specified.

[0020] The research basis of the present invention is to carry out whole genome sequencing on the existing glutamic acid temperature-sensitive mutant strain obtained by mutagene...

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Abstract

The invention provides a temperature-sensitive recombinant corynebacterium glutamicum producing glutamic acid. The temperature-sensitive recombinant corynebacterium glutamicum is obtained by transforming wild type corynebacterium glutamicum ( C. glutamicum ATCC 13032) and knocking out two key genes in a genome of the corynebacterium glutamicum in a peptidoglycan synthesis route, namely UDP-N-enol form acetyl glucosamine transferase gene (murA) and UDP-N-acetyl cell wall acidohydrogenase gene (murB), the glutamic acid yield of the recombinant corynebacterium glutamicum is improved obviously, and the glutamic acid yield is 4-7 g/L under the condition that the fermentation temperature rises to 38.5 DEG C.

Description

technical field [0001] The invention belongs to the field of genetic engineering and fermentation engineering, and particularly relates to molecular transformation of Corynebacterium glutamicum ATCC13032 by genetic engineering technology to obtain glutamic acid-producing thermosensitive recombinant bacteria. Background technique [0002] Glutamic acid is one of the basic amino acids for nitrogen metabolism in living organisms, and it is of great significance in metabolism. It is widely used in food, medicine and industry. In order to increase the yield of glutamic acid, the most prominent research work on glutamic acid-producing bacteria is the breeding of high-yielding glutamic acid bacteria. The wild strains of glutamic acid-producing bacteria isolated from nature often cannot meet the requirements of fermentation production development in terms of production performance. Glutamic acid production strains have a large natural variation ability, and the negative variation ...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12P13/14C12R1/15
CPCC12N9/0006C12N9/1096C12P13/14C12Y101/01158
Inventor 谢希贤陈宁石拓马跃超徐庆阳张成林李燕军
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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