Temperature-sensitive recombinant corynebacterium glutamicum producing glutamic acid and application thereof

Abstract

The invention provides a temperature-sensitive recombinant corynebacterium glutamicum producing glutamic acid. The temperature-sensitive recombinant corynebacterium glutamicum is obtained by transforming wild type corynebacterium glutamicum ( C. glutamicum ATCC 13032) and knocking out two key genes in a genome of the corynebacterium glutamicum in a peptidoglycan synthesis route, namely UDP-N-enol form acetyl glucosamine transferase gene (murA) and UDP-N-acetyl cell wall acidohydrogenase gene (murB), the glutamic acid yield of the recombinant corynebacterium glutamicum is improved obviously, and the glutamic acid yield is 4-7 g / L under the condition that the fermentation temperature rises to 38.5 DEG C.

Description

technical field [0001] The invention belongs to the field of genetic engineering and fermentation engineering, and particularly relates to molecular transformation of Corynebacterium glutamicum ATCC13032 by genetic engineering technology to obtain glutamic acid-producing thermosensitive recombinant bacteria. Background technique [0002] Glutamic acid is one of the basic amino acids for nitrogen metabolism in living organisms, and it is of great significance in metabolism. It is widely used in food, medicine and industry. In order to increase the yield of glutamic acid, the most prominent research work on glutamic acid-producing bacteria is the breeding of high-yielding glutamic acid bacteria. The wild strains of glutamic acid-producing bacteria isolated from nature often cannot meet the requirements of fermentation production development in terms of production performance. Glutamic acid production strains have a large natural variation ability, and the negative variation ...

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Problems solved by technology

The wild strains of glutamic acid-producing bacteria isolated from nature often cannot meet the requirements of fermentation production development in terms of production performance

Glutamic acid production strains have a large natural variation ability, and the negative variation frequency is usually higher than the positive variation frequency, resulting in the aging of the strain and the decline of the strain vitality, resulting in low acid production rate, low acid production rate, and Low sugar-acid conversion rate, high cost, high energy consumption and other bottleneck problems, so the breeding of excellent production strains has always been one of the main research topics in the glutamic acid fermentation industry

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