Detection reagent kit for rapidly detecting poultry disease viruses and detection method of detection reagent kit

A detection kit and detection method technology, applied in the fields of spectroscopy and molecular diagnosis, can solve problems such as high cost, and achieve the effects of short time-consuming, easy operation and good application prospects

Active Publication Date: 2016-05-25
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problem that existing detection technology requires expensive instruments to be equipped with professional technicians, the primary purpose of the present invention is to provide a detection kit for rapid detection of poultry disease virus

Method used

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  • Detection reagent kit for rapidly detecting poultry disease viruses and detection method of detection reagent kit
  • Detection reagent kit for rapidly detecting poultry disease viruses and detection method of detection reagent kit
  • Detection reagent kit for rapidly detecting poultry disease viruses and detection method of detection reagent kit

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Embodiment 1

[0032] The invention provides a detection kit for rapid detection of poultry disease virus, which comprises a metal sol solution, a quartz plate and a Raman spectrometer.

[0033] The metal sol solution is silver sol solution.

[0034] The preparation method of described silver sol is as follows: under normal temperature, 2.5mL10 -2 mol / LAgNO 3 Add to 75mL distilled water, add 5mLof10 -2 mol / L thioglycolic acid. After stirring for 10min, drop in 2.5mL10 -2 mol / LNaI, after stirring for 20min, a light green AgI sol was obtained, adding 20mgNaBH 4 Reduction, continue to stir for about 10 minutes to generate yellow-brown silver sol, and the obtained spherical particles are uniform in shape, with an average particle diameter of about 35nm and a concentration of about 120μg / mL.

[0035] The quartz plate has a wavenumber range of 700-2000cm -1 Substrate quartz plate without obvious Raman peaks.

[0036] Described Raman spectrometer is SmartRamanOcean5pro portable Raman spectro...

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Abstract

The invention discloses a detection reagent kit for rapidly detecting poultry disease viruses and a detection method of the detection reagent kit and belongs to the field of spectroscopy and molecular diagnosis. According to the detection reagent kit and the detection method, silver sol prepared through a hydroxylamine hydrochloride complexation method is adopted as a Raman enhancement reagent; after the silver sol and an object to be detected are mixed evenly at a certain proportion, the mixture is dripped onto a quartz plate; after natural air drying, Raman detection is carried out with a SmartRaman Ocean5pro portable Raman spectrometer; after principal component analysis and linear discriminant analysis are carried out on an obtained Raman spectrum, qualitative detection of the target pathogen is achieved. The detection reagent kit is easy and convenient to operate and high in sensitivity and has broad application prospects.

Description

technical field [0001] The invention belongs to the fields of spectroscopy and molecular diagnosis, and in particular relates to a detection kit for rapidly detecting poultry disease virus and a detection method thereof. Background technique [0002] Newcastle Disease Virus (NDV), commonly known as Asian chicken plague, can cause diarrhea, dyspnea and neurological symptoms in chickens. Avian influenza virus (Avian Influenza Virus, AIV) includes multiple subtypes, among which the H5 subtype avian influenza virus can cause a large number of morbidity and death in poultry flocks, and is listed as a class A infectious disease by the World Organization for Animal Health and is highly valued. Actively monitoring and controlling the spread of Newcastle disease virus and avian influenza virus in poultry flocks can not only reduce the loss of the farming industry, but also greatly reduce the risk of avian influenza virus transmission from poultry flocks to humans, which has important...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70
CPCC12Q1/04
Inventor 任涛冯敏莎谭阳通
Owner SOUTH CHINA AGRI UNIV
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