Para hydroxybenzene alanine urine detection kit and preparation method thereof

A technology of hydroxyphenylalanine and a detection kit, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problem of insufficient diazotization reaction activity and electron donating Reduced capacity, reduced electron cloud density, etc., to achieve high stability and sensitivity, facilitate mass production, and simple and easy-to-operate preparation methods

Active Publication Date: 2016-05-25
张凤华
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Problems solved by technology

For example, firstly, the monohydric phenol in the urine first reacts with the mercury salt in the reagent A to form a complex, and this complex is due to the hydroxyl group in the monohydric phenol donating its lone pair of electrons to the empty orbital of the metal ion, As a result, the ability of the hydroxyl group to donate electrons to the aromatic ring decreases, and the complexed structure reduces the electron cloud density on the aromatic ring, which eventually leads to insufficient activity in the subsequent diazotization reaction and ultimately leads to inaccurate detection; in addition, reagent B The nitrous acid in the solution is easy to decompose during storage and transportation, which makes it unable to react with reagent C to form diazonium salt, which affects the accuracy of detection

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  • Para hydroxybenzene alanine urine detection kit and preparation method thereof

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preparation example Construction

[0040] The preparation method of p-hydroxyphenylalanine urine detection kit of the present invention comprises the following steps:

[0041] Prepare reagent A: add mineral acid to water to obtain an aqueous solution of mineral acid with a concentration of 0.01-0.5 mol / L;

[0042] Prepare reagent B: add sodium nitrite to water to obtain an aqueous sodium nitrite solution with a concentration of 0.01-0.5 mol / L;

[0043] Preparation of reagent C: adding aniline derivatives and inorganic acids to water to obtain an aqueous inorganic acid solution of aniline derivatives. The benzene ring in the aniline derivatives contains at least nitro, cyano or fluorine groups. The concentration of aniline derivatives in reagent C is 0.005~0.1mol / L, the concentration of inorganic acid is 0.01~0.5mol / L;

[0044] Preparation of reagent D: Add N-1-naphthylethylenediamine dihydrochloride to water to obtain an aqueous solution of N-1-naphthylethylenediamine dihydrochloride, N-1-naphthylethylenediami...

Embodiment 1

[0048] Prepare reagent A: add 0.42ml of concentrated hydrochloric acid to 50ml of water, dilute with water to a total volume of 100ml, and shake fully to obtain a 0.05mol / L hydrochloric acid aqueous solution;

[0049] Prepare reagent B: Add 0.14g of sodium nitrite to 50ml of water, add water to dilute to a total volume of 100ml, shake fully until the sodium nitrite is completely dissolved, and obtain a 0.02mol / L sodium nitrite aqueous solution;

[0050] Preparation of reagent C: Add 0.17g of p-aminobenzenesulfonic acid and 0.17ml of concentrated hydrochloric acid into 50ml of water in sequence, add water to dilute to a total volume of 100ml, and shake fully to obtain an aqueous hydrochloric acid solution of p-aminobenzenesulfonic acid. The concentration is 0.01mol / L, and the concentration of hydrochloric acid is 0.02mol / L;

[0051] Preparation of reagent D: add 0.52g of N-1-naphthylethylenediamine dihydrochloride to 50ml of water, dilute with water to a total volume of 100ml, ...

Embodiment 2

[0053] Prepare reagent A: slowly add 0.22ml of concentrated sulfuric acid into 50ml of water, add water to dilute to a total volume of 100ml, shake fully to obtain 0.04mol / L sulfuric acid aqueous solution;

[0054] Preparation of reagent B: add 0.21g of sodium nitrite to 50ml of water, dilute with water to a total volume of 100ml, shake fully until the sodium nitrite is completely dissolved, and obtain a 0.03mol / L sodium nitrite aqueous solution;

[0055] Prepare reagent C: add 0.27g p-nitroaniline and 0.11ml concentrated sulfuric acid to 50ml water in turn, add water to dilute to a total volume of 100ml, and shake fully to obtain a sulfuric acid aqueous solution of p-nitroaniline, wherein the concentration of p-nitroaniline is 0.02 mol / L, the concentration of sulfuric acid is 0.02mol / L;

[0056] Preparation of reagent D: Add 0.77g of N-1-naphthylethylenediamine dihydrochloride to 50ml of water, dilute with water to a total volume of 100ml, and shake fully to obtain 0.03mol / L ...

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Abstract

The invention provides a para hydroxybenzene alanine urine detection kit. The para hydroxybenzene alanine urine detection kit comprises a reagent A, a reagent B, a reagent C and a reagent D which are stored in four containers respectively. The reagent A is an inorganic acid water solution with the concentration of 0.01-0.5 mol / L. The reagent B is a sodium nitrite water solution with the concentration of 0.01-0.5 mol / L. The reagent C is an inorganic acid water solution of an aniline derivative with the concentration of 0.005-0.1 mol / L, the concentration of inorganic acid ranges from 0.01-0.5 mol / L, and a benzene ring of the aniline derivative at least comprises a sulfo group or a nitro group or a cyano group or a fluorine group. The reagent D is a water solution of N-1-naphthyl ethylenediamine dihydrochloride, and the concentration of N-1-naphthyl ethylenediamine dihydrochloride is 0.01-0.3 mol / L. The concentration of the aniline derivative in the reagent C is not higher than that of inorganic acid in the reagent A and that of sodium nitrite in the reagent B. Inorganic acid is selected from hydrochloric acid or sulfuric acid or tetrafluoroboric acid or hexafluorophosphoric acid. The invention further provides a preparation method of the kit. The detection kit has the good stability, reliability and broad spectrum.

Description

technical field [0001] The invention relates to an in vitro detection kit for clinical diagnosis, in particular to a p-hydroxyphenylalanine urine detection kit and a preparation method. Background technique [0002] Cancer mortality ranks first among all diseases and is the number one killer of human health. It has become a major cause of death that threatens human life and health. According to statistics, the number of cancer patients will double every 25 years. Not only that, the number of deaths from cancer is also rising rapidly around the world, and this number may increase to 13.2 million in 2030. [0003] Early detection is the key to the treatment of tumors. The development of tumors is divided into five stages: benign, tumor cell initiation, early tumor, mid-tumor, and late stage. The frightening thing about cancer is that the first three stages cannot be detected by imaging. Even in the middle stage of the tumor, only very professional doctors can detect it with a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 张凤华
Owner 张凤华
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