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A kind of method that improves silver ion to biofilm removal and bactericidal efficiency

A technology of bactericidal efficiency, biofilm, applied in the fields of botanical equipment and methods, chemicals for biological control, chemical instruments and methods, etc.

Inactive Publication Date: 2019-04-23
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no domestic and foreign literature reports on the use of bis(3-aminopropyl)amine coupled with silver to strengthen the removal and killing of biofilm microbial cells

Method used

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  • A kind of method that improves silver ion to biofilm removal and bactericidal efficiency
  • A kind of method that improves silver ion to biofilm removal and bactericidal efficiency

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Weigh silver nitrate, mix silver nitrate aqueous solution, silver ion concentration is 1mg / l.

[0018] (2) Weigh bis(3-aminopropyl)amine to prepare bis(3-aminopropyl)amine aqueous solution, the concentration of bis(3-aminopropyl)amine is 1000 μM.

[0019] (3) Using glass slides as biofilm attachment carriers, add slides to culture dishes containing LB medium, inoculate activated sludge mixed microbial flora, shake culture for 48 hours, and pre-cultivate biofilms.

[0020] (4) After taking out the biofilm sample that has been cultured, put it into a flask, add sterile water, the biofilm is submerged in water, and then add a certain amount of silver ion solution and bis(3-aminopropyl)amine to the flask solution, so that the final concentration of silver ions in the system is 0.01 mg / l, and the bis(3-aminopropyl)amine solution is 500 μM, gently shaken and reacted for 2 hours, and the number of viable microorganisms in the reaction system is measured by sampling, and th...

Embodiment 2

[0022] (1) Weigh silver nitrate, mix silver nitrate aqueous solution, silver ion concentration is 1mg / l.

[0023] (2) Weigh bis(3-aminopropyl)amine to prepare bis(3-aminopropyl)amine aqueous solution, the concentration of bis(3-aminopropyl)amine is 1000 μM.

[0024] (3) Using glass slides as biofilm attachment carriers, add slides to culture dishes containing LB medium, inoculate activated sludge mixed microbial flora, shake culture for 48 hours, and pre-cultivate biofilms.

[0025] (4) Take out the cultured biofilm sample and put it into a flask, add sterile water to submerge the biofilm in water, then add bis(3-aminopropyl)amine solution to the flask to make the concentration 500 μM After shaking for 1 hour, add silver ion solution to the system so that the final concentration is 0.05mg / l, shake lightly for 2 hours, and complete the removal and sterilization of biofilm.

Embodiment 3

[0027] (1) Take silver oxide or silver peroxide by weighing, add in 15% ammonium hydroxide solution, adjust pH to be 8, make the silver concentration in the solution be 1mg / l.

[0028] (2) Weigh bis(3-aminopropyl)amine to prepare bis(3-aminopropyl)amine aqueous solution, the concentration of bis(3-aminopropyl)amine is 1000 μM.

[0029] (3) Using glass slides as biofilm attachment carriers, add slides to culture dishes containing LB medium, inoculate activated sludge mixed microbial flora, shake culture for 48 hours, and pre-cultivate biofilms.

[0030] (4) After taking out the biofilm sample that has been cultured, put it into a flask, add sterile water, the biofilm is submerged in water, and then add a certain amount of silver ion solution and bis(3-aminopropyl)amine to the flask solution, so that the final concentration of silver ions in the system is 0.05 mg / l, and the bis(3-aminopropyl)amine solution is 500 μM, and the reaction is gently shaken for 2 hours to complete the ...

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Abstract

The invention relates to a method for improving the biological membrane removal and sterilization efficiency of silver ions, and belongs to the technical field of biological membrane control and sterilization. According to the method, the biological membrane removal and sterilization efficiency of the silver ions is improved by coupling a small molecular substance, namely, bis(3-aminopropyl)amine, which has a dissociation effect on the biological membrane and the silver ions. The method is simple and feasible, wide in application range, low in biotoxicity, environmentally friendly, lasting in effect, and economic and effective. The problem that the efficiency of removing a biological membrane formed by the aggregation of microorganisms and sterilizing of separate silver ions is low is solved; the sterilization efficiency of silver is improved.

Description

technical field [0001] The invention relates to a method for improving the efficiency of inorganic silver ions to remove and sterilize biofilms, and belongs to the technical field of biofilm control and disinfection. Background technique [0002] Biofilm refers to a structured microbial community formed by planktonic bacteria attached to biotic or abiotic surfaces and bound together by their own secreted extracellular polymeric substances (EPS). Biofilms widely exist in the environment, such as disinfection systems, water pipes, industrial pipelines, ventilation equipment, medical equipment, and human tissues and organs under pathological conditions. In the medical field, some pathogenic bacteria form biofilms inside and outside the human body and on the surface of medical materials, which is one of the main causes of recurrent and difficult-to-control chronic infectious diseases, and 80% of human bacterial infections are related to biofilms. In the field of environment, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N59/16A01N33/04A01P1/00C02F1/50
CPCA01N33/04A01N59/16C02F1/50C02F2303/04A01N2300/00
Inventor 全向春武亚川肖竹天
Owner BEIJING NORMAL UNIVERSITY