Mycoplasma anseris in-vitro liquid culture medium and preparation method thereof

Abstract

The invention provides a mycoplasma anseris in-vitro liquid culture medium. The mycoplasma anseris in-vitro liquid culture medium consists of a culture medium A and a culture medium B, wherein the culture medium A is a mycoplasma culture medium for breakdown of glucose; the culture medium B is a mycoplasma culture medium for breakdown of arginine; the culture medium S comprises the following ingredients: mycoplasma broth base, deionized water, glucose, sodium pyruvate, 1% phenol red, a 10% thallium acetate solution, inactivated horse serum, a yeast extract, penicillin, an L-cysteine hydrochloride aqueous solution and a nicotinamide adenine dinucleotide nad aqueous solution; the culture medium B comprises the following ingredients: mycoplasma broth base, deionized water, sodium pyruvate, 1% phenol red, a 10% thallium acetate solution, inactivated horse serum, a yeast extract, penicillin and an L-arginine monohydrochloride aqueous solution. By adoption of the mycoplasma anseris in-vitro liquid culture medium, growth can be realized only after 24 h, and the tilter after 48-h growth can be 10<3> CCU / mL. The mycoplasma anseris in-vitro liquid culture medium is suitable for isolated culture and diagnosis of mycoplasma anseris.

Description

technical field [0001] The invention belongs to the technical field of culture medium, and in particular relates to an in vitro liquid culture medium for Mycoplasma swanensis. Background technique [0002] Mycoplasma geese ( Mycoplasma anseris ) is a pathogen that causes goose respiratory diseases, inflammation of the penis and cloaca, decreased egg production, infertile eggs and increased dead embryos. It was first detected by Kosovac and Djurisic from a goose suffering from salpingitis, then isolated by Palya from the genitals of a male goose in Hungary in 1971, and later named sp.1220 strain (Stipkovits, 1978, 1984 , 1986, 2012). Epidemiological surveys showed that Germany, Poland, Czechoslovakia, the Soviet Union, the United Kingdom and other countries have successively isolated Mycoplasma geese from inflamed male goose genitals and cloaca. my country is the main goose breeding base in the world, and goose meat production accounts for 93% of the global total. Epidemi...

AI Technical Summary

Problems solved by technology

At present, goose mycoplasma disease, duck plague and goose blight are listed as the three major diseases infected by goose flocks in my country. Compared with mycoplasma gallisepticum ( Mycoplasma gallispeticum ) and Mycoplasma synovium ( Mycoplasmasymoviae ), the research on the isolation, diagnosis and prevention and control measures of Mycoplasma geese in my country is still in its infancy. At present, the artificial culture medium used to isolate Mycoplasma geese is generally improved from Frey or PPLO medium, but there are still viable bacterial titers In addition, there are two kinds of mycoplasma with different biochemical properties (one decomposes glucose and the other decomposes arginine) in geese, and it is easy to produce false negatives. Therefore, there is an urgent need in this field for efficient, stable, And it can achieve the rapid separation and diagnosis of Mycoplasma goosei, and then carry out the research on the treatment and prevention of Mycoplasma goosei infection

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