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A method for extracting oligopeptide and oil from fly maggots

A fly maggot and oligopeptide technology, which is applied in the fields of fat oil/fat refining, fat oil/fat production, fat production, etc., can solve the problems of high cost, cumbersome steps, protein damage, etc., and achieve the effect of quality improvement and good saponification value

Active Publication Date: 2019-03-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the solvent method to extract oil, although a high oil extraction rate can be obtained, but after the oil is extracted, a series of refining processes such as deacidification and degumming are required, and the steps are cumbersome and troublesome.
Using the solvent method to extract oil, the workshop also needs to be designed in an explosion-proof mode, which is costly and not suitable for small oil factories
Moreover, insect oil is not currently produced on a large scale like soybeans and rapeseed, so it is not suitable for this method
Although oil extraction by pressing is simple and convenient, it needs high-temperature heat treatment before pressing, and the protein is greatly damaged after pressing, so this method is not economical

Method used

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  • A method for extracting oligopeptide and oil from fly maggots
  • A method for extracting oligopeptide and oil from fly maggots
  • A method for extracting oligopeptide and oil from fly maggots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Fresh maggots are taken by weighing 200g, and after blanching, the mass ratio of fresh maggots to water is 1:1 for refining. Add deionized water to make the solid-liquid ratio 1:10, pour the mixed slurry into the enzymolysis tank, raise the temperature to 55°C, adjust the pH value to 9 with 0.5M sodium hydroxide solution, wait until the pH value is stable After transformation, the amount of Alcalase alkaline protease added is 5000U / g protein, and then the pH value is adjusted with 0.5M sodium hydroxide solution to keep the pH value stable during the enzymolysis process, and the volume of sodium hydroxide solution required is recorded. After 3 hours of enzymatic hydrolysis, the pH value was adjusted to 6 with 1M hydrochloric acid solution. Afterwards, centrifugation was carried out at a speed of 4000 rpm for 20 minutes. The emulsified layer, aqueous phase (enzymolysis solution) and residue were obtained.

[0045] Add 2 g of diatomaceous earth into the water phase, stir...

Embodiment 2

[0049]Fresh maggots are taken by weighing 200g, and after blanching, the mass ratio of fresh maggots to water is 1:1 for refining. Add deionized water to make the solid-liquid ratio 1:15, pour the mixed slurry into the enzymolysis tank, raise the temperature to 45°C, adjust the pH value to 9 with 0.5M sodium hydroxide solution, and wait until the pH value is stable. After transformation, the amount of Alcalase alkaline protease added is 5000U / g protein, and then the pH value is adjusted with 0.5M sodium hydroxide solution to keep the pH value stable during the enzymolysis process, and the volume of sodium hydroxide solution required is recorded. After 3 hours of enzymatic hydrolysis, the pH value was adjusted to 6 with 1M hydrochloric acid solution. Afterwards, centrifugation was carried out at a speed of 4000 rpm for 20 minutes. An emulsion layer, an aqueous phase and a residue are obtained.

[0050] Add 2 g of diatomaceous earth into the water phase, stir evenly, and then ...

Embodiment 3

[0054] Fresh maggots are taken by weighing 400g, and after blanching, the mass ratio of fresh maggots to water is 1:1 for defibrination. Add deionized water to make the solid-liquid ratio 1:15, pour the mixed slurry into the enzymolysis tank, raise the temperature to 55°C, adjust the pH value to 8 with 1M sodium hydroxide solution, and wait until the pH value is stable Finally, the amount of Alcalase alkaline protease added is 5000U / g protein, and then the pH value is adjusted with 1M sodium hydroxide solution to keep the pH value stable during the enzymolysis process, and the volume of sodium hydroxide solution required is recorded. After 3 hours of enzymatic hydrolysis, the pH value was adjusted to 6 with 1.5M hydrochloric acid solution. Afterwards, centrifugation was carried out at a speed of 4000 rpm for 20 minutes. An emulsion layer, an aqueous phase and a residue are obtained.

[0055] Add 4 g of diatomaceous earth into the water phase, stir evenly, and then perform su...

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Abstract

The invention discloses a method for extracting oligopeptide and grease from maggots, and belongs to the technical field of enzymolysis of maggot protein in biotechnology. Technical conditions for maggot enzymolysis and a demulsifying method of an emulsified layer generated after the enzymolysis, which are provided by the invention, provide a new way for deep processing and resource utilization of the maggots. A product obtained in the invention contains 80.60% of polypeptide, and has a certain oxidation resistance and ACE inhibitory activity; according to the method provided by the invention, the use of an organic solvent is avoided, and the quality of the grease is improved to a certain extent.

Description

technical field [0001] The invention relates to a method for extracting oligopeptides and oils from fly maggots, belonging to the technical field of enzymolysis of fly maggot proteins in biotechnology. Background technique [0002] Fly maggots grow extremely fast, and the newly hatched larvae are 1 cm long and weigh about 30 mg after 4-5 days. Fly maggot breeding equipment is simple and easy to operate. And the cultivation of fly maggots is not like other aquaculture industries, there is no epidemic disease, and there is no need to spend great efforts on epidemic prevention and disease prevention. Fly maggots are rich in protein, oil and chitin. The protein content in fly maggots accounts for about 60% of the dry weight. Compared with other insects, the amino acid composition in the protein is more reasonable, the content of essential amino acids is high, and the protein fiber contained is less, which is easier to be digested and absorbed by the human body. The content of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C11B1/02C11B3/00
CPCC11B1/02C11B1/025C11B3/00C12P21/06
Inventor 杨严俊苏宇杰董柘
Owner JIANGNAN UNIV
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