Mycelium growth activity determining method

Abstract

The invention provides a mycelium growth activity determining method, belongs to the technical field of an activity determining method, and particularly relates to the mycelium growth activity determining method. The invention provides the mycelium growth activity determining method with the advantages that the operation is simple, and the determining is reliable. The mycelium growth activity determining method comprises the following steps of adding a certain quantity of mother liquor into a PDA (Potato Dextrose Agar) culture medium; sufficiently mixing the mother liquor and the PDA culture medium; uniformly pouring the mixed culture medium into a culture dish; performing condensation to obtain a virus-carrying culture medium; after the culture medium is cooled and solidified, transplanting prepared bacterium cake onto the virus-carrying culture medium in a back-side attaching way by an inoculating needle or a pair of disinfected tweezers; and using a negative control, a blank control and a positive control.

Description

technical field [0001] The invention belongs to the technical field of activity determination methods, in particular to a method for determination of mycelia growth activity. Background technique [0002] Effective, rapid and sensitive evaluation of antibacterial activity of candidate compounds is an important link in the screening and development of fungicides. For the screening of antibacterial and antifungal active ingredients, the commonly used antibacterial activity determination methods mainly include: drug diffusion method (such as perforated agent diffusion method, filter paper method, cup and saucer method), growth rate method (such as poisoned plate method, liquid suspension culture method, porous plate turbidimetric method), spore germination method, spectrophotometry by detecting cell enzyme activity Each of these methods has advantages, but also has certain limitations. Contents of the invention [0003] The present invention aims at the above problems and pr...

AI Technical Summary

Problems solved by technology

[0002] Effective, rapid and sensitive evaluation of antibacterial activity of candidate compounds is an important link in the screening and development of fungicides. For the screening of antibacterial and antifungal active ingredients, the commonly used antibacterial activity determination methods mainly include: drug diffusion method (such as perforated reagent diffusion method, filter paper method, cup and saucer method), growth rate method (such as poisoned plate method, liquid suspension culture method, porous plate turbidimetric method), spore germination method, spectrophotometry by detecting cell enzyme activity These methods have their own advantages, but also have certain limitations.