Method for preparing immobilized microorganisms using graphene oxide modified calcium alginate
An immobilized microorganism and graphene modification technology, applied in the field of environmental science, can solve the problems of difficult to treat with high-salt wastewater, poor mechanical strength, etc., and achieve the effects of excellent biological adsorption performance, strong adaptability and simple operation.
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Embodiment 1
[0029] Example 1: Preparation of immobilized Saccharomyces cerevisiae using graphene oxide modified calcium alginate
[0030] (1) After mixing 1g of sodium alginate, 0.01g of graphene oxide and 100mL of deionized water, heat it in a water bath until it completely dissolves, then let it stand and cool to 40°C to obtain a sodium alginate-graphene oxide mixed solution;
[0031] (2) Weigh 2g of waste Saccharomyces cerevisiae powder (through a 40-200 mesh sieve) from the brewery and fully mix it with the sodium alginate-graphene oxide mixed solution to obtain a mixed solution containing Saccharomyces cerevisiae;
[0032] (3) Prepare CaCl with deionized water 2 solution to make CaCl 2 The concentration reaches 4%, obtains cross-linking agent solution;
[0033] (4) Drop the mixed solution containing Saccharomyces cerevisiae into the crosslinking agent solution with a syringe or a dropper to form spherical particles, and soak in it for 2-10 hours;
[0034] (5) The formed spherical ...
Embodiment 2
[0036] Example 2: Kinetic determination of uranium adsorption by immobilized Saccharomyces cerevisiae prepared separately from calcium alginate and graphene oxide modified calcium alginate
[0037] In a solution with a uranium concentration of 9.2 mg / L, 1 g / L immobilized Saccharomyces cerevisiae prepared by graphene oxide-modified calcium alginate and 1 g / L calcium alginate prepared immobilized Saccharomyces cerevisiae were respectively added, and then The pH value was 5-6, and the reaction temperature was 0-12h at 25°C; it was found that the immobilized S. Immobilized Saccharomyces cerevisiae, stable adsorption of uranium, no desorption, see the results figure 1 .
[0038] The pseudo-second-order kinetic equation fitting further proved that the initial adsorption rate of the immobilized Saccharomyces cerevisiae prepared by graphene oxide-modified calcium alginate was 9.6×10 -2 mg / (gmin), the latter is 9.4×10 -2 mg / (g min). Apparently, graphene oxide improved the kinetics ...
Embodiment 3
[0039] Example 3: Adsorption determination of uranium by immobilized Saccharomyces cerevisiae prepared separately from calcium alginate and calcium alginate modified by graphene oxide
[0040] In the solution that the uranium concentration is 1.5-36.5mg / L, add the immobilized Saccharomyces cerevisiae prepared by 1g / L calcium alginate modified by graphene oxide and the immobilized Saccharomyces cerevisiae prepared by 1g / L calcium alginate respectively, and then The initial pH value was 5-6 and the temperature was 25°C for 12 hours; it was found that the immobilized S. The adsorption capacity of each is 27.4, 25.5mg / g.
[0041] When the initial pH values of the solution were 3 and 7 respectively, and the initial concentration of uranium was 12mg / L, after 12 hours of reaction, it was found that graphene oxide could enhance the adsorption of uranium by calcium alginate-immobilized Saccharomyces cerevisiae. figure 2 .
[0042] Determination of unimmobilized original Saccharomy...
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