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A method for extracting cyclic (glycerol-tyrosine) dipeptide from bacillus coagulans

A technology of Bacillus coagulans and seeds, which is applied in the field of preparation of cyclic dipeptides, and achieves the effects of high purity, simple extraction method and low cost

Inactive Publication Date: 2019-01-04
HUANGHE S & T COLLEGE
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  • Application Information

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Problems solved by technology

However, there are no reports of small molecule cyclic dipeptides in the metabolites of Bacillus coagulans. Cyclic dipeptides are diketopiperazines with a stable six-membered ring structure, which exhibit antibacterial, antifungal, anti Viral, anti-tumor, immunosuppressive, neuroprotective, anti-hyperglycemic, anti-malarial and other biological and pharmacological activities

Method used

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  • A method for extracting cyclic (glycerol-tyrosine) dipeptide from bacillus coagulans
  • A method for extracting cyclic (glycerol-tyrosine) dipeptide from bacillus coagulans

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Embodiment 1

[0032] A method for extracting cyclic (glycerol-tyrosine) dipeptide from Bacillus coagulans, including the following steps:

[0033] (1) Strain activation: inoculate Bacillus coagulans on a plate medium and cultivate it at 37°C for 24 hours to obtain activated strains;

[0034] (2) Primary culture: inoculate the activated strain obtained in step (1) into MRS liquid medium, and culture at 150r / min, 37℃ for 12 hours on a shaker to obtain seed liquid;

[0035] (3) Secondary culture: Inoculate the seed liquid obtained in step (2) into MRS liquid medium, the volume of the seed liquid is 4% of the volume of MRS liquid medium, at 150r / min, 37℃, shaker Cultivate for 48 hours to obtain fermentation broth;

[0036] (4) Preparation of crude extract: the fermentation broth prepared in step (3) was concentrated by rotary evaporation to 1 / 4 of the original volume and centrifuged, the supernatant was taken out and precipitated with ethanol, filtered and the filtrate continued to be concentrated to 1...

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Abstract

The invention discloses a method for extracting dipeptide cyclo(Gly-Tyr) from bacillus coagulans. A method for preparing dipeptide cyclo(Gly-Tyr) mainly comprises steps as follows: activating and culturing bacteria, acquiring a crude extract, performing course separation on the crude extract through silica gel column chromatography, fine separation through gel column chromatography and further purification through HPLC, performing TLC tracking and detection and identifying an acquired compound to be dipeptide cyclo(Gly-Tyr) through nuclear magnetic detection and comparison with literature. The raw material bacillus coagulans is a probiotic and is safe and harmless, and an extraction process is simple and low in cost; the prepared compound has high purity.

Description

Technical field [0001] The invention belongs to the technical field of preparation of cyclic (glycol-tyrosine) dipeptide, and specifically relates to a method for extracting cyclic (glycol-tyrosine) dipeptide from Bacillus coagulans. Background technique [0002] Bacillus coagulans (bacillus coagulans LL1103), also known as spore lactic acid bacteria, not only has the properties of lactic acid bacteria to produce lactic acid, but also has the rich enzyme system of bacillus and the characteristics of strong stress resistance, high temperature resistance, bile salt resistance, and easy storage. As a rising star of the probiotic family, making it into microecological preparations has been widely used in aquaculture, animal husbandry, food and medicine industries. In addition to its health care effect in maintaining intestinal microecological balance, Bacillus coagulans has obvious effects on common intestinal diseases such as chronic colitis, diarrhea and constipation. It also has t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/04C07D241/08C12R1/07
CPCC07D241/08C12P21/02
Inventor 陈林郭庆丰马经纬康文艺
Owner HUANGHE S & T COLLEGE
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