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Method for producing L-2-aminobutyric acid by fermentation process

A technology of aminobutyric acid and fermentation method, applied in the field of microbial fermentation, can solve the problems of loss of competitiveness, high production cost, expensive raw materials, etc., and achieve the effect of improving production capacity and efficiency, high social and economic benefits, and simple extraction process

Inactive Publication Date: 2016-06-15
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional chemical methods, whether organic synthesis or chemical resolution, lose their competitiveness due to high production costs
In the biological method, the efficiency of the enzyme-catalyzed method is relatively high, but there are still problems of cumbersome operation, expensive raw materials, and high production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Put the bacteria into the seed tank and cultivate under the following conditions: culture temperature 36°C, pH 7.0, dissolved oxygen controlled at 25%, transplantation OD 10, and mature bacteria were obtained;

[0020] Seed tank formula glucose 30g / L, yeast powder 5g / L, peptone 6g / L, ammonium sulfate 2.5g / L, potassium dihydrogen phosphate 4g / L, magnesium sulfate 0.5g / L, VB110mg / L, VH20mg / L, sulfuric acid Ferrous iron 75mg / L, manganese sulfate 100mg / L, defoamer 0.25ml / L, and the rest is water.

[0021] (2) Put the mature strains into the fermenter and cultivate them under the following conditions: Fermentation through liquid deep ventilation, in the early stage of fermentation, that is, before culturing for 15 hours, through microporous filtration sterilization technology, pour into the fermentation medium One-time input of IPTG (isopropyl-β-D-thiogalactoside) with a final concentration of 0.1mmol / L, the temperature of the fermentation process is controlled in two st...

Embodiment 2

[0025] (1) Put the bacteria into the seed tank and cultivate under the following conditions: culture temperature 36°C, pH 7.0, dissolved oxygen controlled at 25%, transplantation OD 10, and mature bacteria were obtained;

[0026] Seed tank formula glucose 20g / L, yeast powder 5g / L, peptone 6g / L, ammonium sulfate 2.5g / L, potassium dihydrogen phosphate 4g / L, magnesium sulfate 0.5g / L, VB110mg / L, VH20mg / L, sulfuric acid Ferrous iron 75mg / L, manganese sulfate 100mg / L, defoamer 0.25ml / L, and the rest is water.

[0027] (2) Put the mature strains into the fermenter, and cultivate them under the following conditions: ferment through liquid deep ventilation, and feed-batch technology, and add the required amount of IPTG (final concentration of 0.1 to 0.3 After the addition of mmol / L IPTG (isopropyl-β-D-thiogalactoside) is completed, the temperature of the fermentation process is controlled in two stages, and the temperature is controlled at 35°C in the early stage of fermentation. Cult...

Embodiment 3

[0031] (1) Put the strains into the seed tank and cultivate them under the following conditions: culture temperature 33°C, pH 6.5, dissolved oxygen controlled at 20%, transplantation OD 20, to obtain mature strains;

[0032] Seed tank formula glucose 20g / L, yeast powder 5g / L, peptone 6g / L, ammonium sulfate 2.5g / L, potassium dihydrogen phosphate 4g / L, magnesium sulfate 0.5g / L, VB 1 10mg / L, VH20mg / L, ferrous sulfate 75mg / L, manganese sulfate 100mg / L, defoamer 0.25ml / L, and the rest is water.

[0033] (2) Put the mature strains into the fermenter and cultivate them under the following conditions: Fermentation through liquid deep ventilation, in the early stage of fermentation, that is, before culturing for 15 hours, through microporous filtration sterilization technology, pour into the fermentation medium One-time input of IPTG (isopropyl-β-D-thiogalactoside) with a final concentration of 0.3mmol / L, the temperature of the fermentation process is controlled in two stages, the temp...

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Abstract

The invention relates to a method for producing L-2-aminobutyric acid by fermentation, comprising the following steps: (1), inserting the strain into a seed tank to obtain a mature strain; (2), adding the mature strain Connect it into a fermenter for fermentation and cultivation; (3) After the fermentation is over, the fermentation liquid is first sterilized by a ceramic membrane, then passed through an ultrafiltration membrane with a molecular weight of 2-4Kd, and decolorized by activated carbon, and the 732 ion exchange resin is used Adsorbed and eluted with ammonia water, evaporated and concentrated to obtain the crude product of L-2-aminobutyric acid. The method for producing L-2-aminobutyric acid of the present invention, through the improvement of fermentation formula and process control, microbial enzyme conversion scheme and control process, extraction scheme and control process, makes the extraction process simple and the product purity can reach more than 95%, The production capacity and efficiency of L-2-aminobutyric acid are greatly improved, which can meet the large-scale industrial production of L-2-aminobutyric acid, and have higher social and economic benefits.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for producing L-2-aminobutyric acid by fermentation. Background technique [0002] 2-Aminobutyric acid is a natural amino acid that inhibits the transmission of human nerve information. It is also an important chemical raw material and pharmaceutical intermediate. It has been widely used in drug synthesis, such as the synthesis of anti-tuberculosis drug ethambutol hydrochloride, L-2- GABA solution is the main raw material for the synthesis of the new antiepileptic drug levetiracetam. [0003] Limited by technology and cost, the output of L-2-aminobutyric acid in my country cannot meet the demand of domestic and foreign trade export. There are chemical and biological methods for the preparation of L-2-aminobutyric acid. JefferyEA et al. use mercury electrodes to electrocatalytically reduce their corresponding keto amino acids (or sodium salts of amino acid...

Claims

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Application Information

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IPC IPC(8): C12P13/04
CPCC12P13/04
Inventor 曹华杰刘帅李静岳贵龙张孟涛
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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