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A method for detecting dopamine using carbon dots as fluorescent probes

A fluorescent probe, dopamine technology, applied in the field of biosensing, can solve the problems of low sensitivity, the sensitivity cannot meet the clinical requirements of dopamine, etc., and achieve the effect of good selectivity

Active Publication Date: 2018-01-30
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The sensitivity of the above methods cannot meet the clinical requirements of dopamine detection, so lower sensitivity can be achieved, and the method of rapid detection of dopamine has become a research hotspot

Method used

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  • A method for detecting dopamine using carbon dots as fluorescent probes
  • A method for detecting dopamine using carbon dots as fluorescent probes
  • A method for detecting dopamine using carbon dots as fluorescent probes

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0028] Synthesis of embodiment 1 carbon dot fluorescent probe 1

[0029] Weigh 3-aminophenylboronic acid powder and place it in a small beaker, add 20mL deionized water and stir until the solid is completely dissolved to obtain an aqueous precursor solution; transfer the uniformly dispersed solution to a polytetrafluoroethylene hydrothermal reaction kettle, The reaction was carried out at 150°C for 8 hours to obtain a brownish-yellow solution of carbon dots; the obtained brownish-yellow solution was filtered to remove large particles of impurities; after that, the initial product solution was rotary evaporated to obtain carbon dots with in-situ bifunctionalization of borate and amino groups Solid powder, the obtained carbon dots are fluorescent probes, the molar ratio of carbon: boron: nitrogen in the carbon dots is 10:5:2; the obtained carbon dot powder is characterized by a transmission electron microscope, and its particle size ranges from 2nm to 5nm ; The wavelength of the...

Embodiment 2

[0030] Synthesis of embodiment 2 carbon dot fluorescent probe 2

[0031] Weigh 3-aminophenylboronic acid powder and place it in a small beaker, add 20mL deionized water and stir until the solid is completely dissolved to obtain an aqueous precursor solution; transfer the uniformly dispersed solution to a polytetrafluoroethylene hydrothermal reaction kettle, The reaction was carried out at 150°C for 16 hours to obtain a brownish-yellow solution of carbon dots; the obtained brownish-yellow solution was filtered to remove large particles of impurities; after that, the initial product solution was rotary evaporated to obtain carbon dots with in-situ bifunctionalization of borate and amino groups Solid powder, the obtained carbon dots are fluorescent probes, the molar ratio of carbon: boron: nitrogen in the carbon dots is 30:7:4; the obtained carbon dot powder is characterized by transmission electron microscopy, and its particle size ranges from 1nm to 6nm ; The fluorescence emiss...

Embodiment 3

[0032] Synthesis of embodiment 3 carbon dot fluorescent probe 3

[0033] Weigh 3-aminophenylboronic acid powder and place it in a small beaker, add 20mL deionized water and stir until the solid is completely dissolved to obtain an aqueous precursor solution; transfer the uniformly dispersed solution to a polytetrafluoroethylene hydrothermal reaction kettle, The reaction was carried out at 250°C for 8 hours to obtain a brownish-yellow solution of carbon dots; the obtained brownish-yellow solution was filtered to remove large particles of impurities; after that, the initial product solution was rotary evaporated to obtain carbon dots with in-situ bifunctionalization of borate and amino groups Solid powder, the obtained carbon dots are fluorescent probes, the molar ratio of carbon: boron: nitrogen in the carbon dots is 42:5:4; the obtained carbon dot powders are characterized by transmission electron microscopy, and the particle size range is 3nm-5nm ; The fluorescence emission p...

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Abstract

The invention discloses a method for detecting dopamine by using carbon dots as a fluorescence probe. The method comprises the following steps: preparation of a fluorescence probe, establishment of a standard curve and determination of dopamine concentration. Carbon dots with high luminescent efficiency are used as the fluorescence probe, and trace amount of dopamine is efficiently detected by the fluorescence enhancement of dopamine on the carbon dots. The detection limit reaches 0.1 pM, detection sensitivity is high, and selectivity is good.

Description

technical field [0001] The invention relates to the field of biosensing. More specifically, it relates to a method for detecting dopamine using carbon dots as fluorescent probes. Background technique [0002] Dopamine is a catecholamine that acts as an important neurotransmitter in the human body, and its content in the human body is closely related to human behavior. The concentration of this substance directly affects the learning and memory ability of human beings. High or low levels of dopamine may cause a series of diseases. The content of dopamine in patients with Parkinson's disease is very low, less than 10 -10 mol / L. The determination of dopamine content has great significance in physiological function and clinical aspects. There are many methods for the detection of dopamine reported so far. Among them, the detection cost of high-performance liquid chromatography is high, and the sensitivity is low (J.Chromatogr.2000,179-189), and visible light spectrophotome...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/645
Inventor 周树云刘晓静谢政胡秀杰严峻孙兴明
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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