Method for extracting ergosterol from Armillaria chrysanthemum and its application

A technology of Armillaria chrysanthemum and Armillaria chrysogenum, which is applied in the field of preparation of anti-liver cancer drugs, can solve the problems of ergosterol purity requirements gap, cumbersome steps, time-consuming, etc., and achieve large amount of preparation and simple process , The effect of small consumption of reagents

Active Publication Date: 2017-08-01
TIANJIN YAOYU BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The Chinese invention patent with the authorized publication number CN102659890B discloses a method for extracting ergosterol from Daben. It takes a series of steps such as extraction, concentration, cracking, lipophilic extraction, saponification, catalytic extraction and purification to successfully extract ergosterol. , but this method is not only cumbersome and time-consuming, but also the purity of ergosterol can only reach 80%, which still has a gap in the purity requirements of ergosterol as a pharmaceutical component

Method used

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  • Method for extracting ergosterol from Armillaria chrysanthemum and its application
  • Method for extracting ergosterol from Armillaria chrysanthemum and its application
  • Method for extracting ergosterol from Armillaria chrysanthemum and its application

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Embodiment 1

[0040] The present embodiment extracts the method for ergosterol from Armillaria chrysanthemum, comprises the following steps:

[0041] (1) Armillaria chrysanthemum sporocarp was used as raw material, dried in the shade at room temperature, dried and dehydrated at 60°C, and then ultrafinely pulverized at -20°C for 5 minutes to obtain superfine powder of Armillaria chrysanthemum sporocarp.

[0042] (2) Take 1Kg of the above-mentioned Armillaria chrysanthemum fruiting body superfine powder, add 6.0L analytical pure water to extract 3 times at 90°C, each time for 1h, and centrifuge at 8000rpm to collect the supernatant and precipitate respectively, repeat 3 times, the obtained The precipitate was dried in an oven at 60°C.

[0043] (3) Add 2 times the volume of analytical grade acetone to the above dry precipitate and extract by cold soaking for 5 hours, centrifuge at 4000rpm to collect the supernatant, concentrate under reduced pressure to a paste, and obtain the small molecule ext...

Embodiment 2

[0051] The present embodiment extracts the method for ergosterol from Armillaria chrysanthemum, comprises the following steps:

[0052] (1) Armillaria chrysanthemum fruiting bodies were dried in the shade at room temperature, dried and dehydrated at 40°C, and then ultrafinely pulverized at -10°C for 5 minutes to obtain superfine powder of Armillaria chrysanthemum fruiting bodies.

[0053] (2) Take 1Kg of the above-mentioned Armillaria chrysanthemum fruiting body superfine powder, add 10.0L analytical pure water to extract 3 times at 80°C, each time for 1h, and centrifuge at 5000rpm to collect the supernatant and precipitate respectively, repeat 3 times. The resulting supernatant was concentrated under reduced pressure to a paste for storage, and the resulting precipitates were combined and put into an oven for drying at 70°C.

[0054] (3) Add 3 times the volume of analytical grade acetone to the above dry precipitate and extract by cold soaking for 5 hours, and centrifuge at 5...

Embodiment 3

[0060] The present embodiment extracts the method for ergosterol from Armillaria chrysanthemum, comprises the following steps:

[0061] (1) Armillaria chrysanthemum fruiting bodies were dried in the shade at room temperature, dried and dehydrated at 50°C, and then ultrafinely pulverized at -15°C for 10 minutes to obtain Armillaria chrysanthemum fruiting body superfine powder.

[0062] (2) Take 1Kg of the above-mentioned Armillaria chrysanthemum fruiting body superfine powder, add 15L analytical pure water to extract 3 times at 75°C for 2 hours each time, and centrifuge at 6000rpm to collect the supernatant and precipitate respectively, repeat 3 times. The precipitates were combined and placed in an oven for drying at 80°C.

[0063] (3) Add 5 times the volume of analytical grade acetone to the above dry precipitate and extract by cold soaking for 8 hours, and centrifuge at 6000rpm to collect the supernatant, concentrate under reduced pressure to a paste, and obtain the small mo...

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Abstract

A method of extracting ergosterol from armillaria luteo-virens is provided. The method includes subjecting a fruiting body of the armillaria luteo-virens to superfine grinding, adding acetone, performing cold leaching, extracting, filtering to obtain a supernatant liquid, subjecting the supernatant liquid to vacuum concentration, dissolving, and separating through a Silica normal-phase silica gel chromatographic column, wherein a binary mobile phase adopting n-hexane as an A phase and ethyl acetate as a B phase is utilized, elution is performed according to a manner that the mobile phase in which the concentration of the A phase is 100% is adopted from 0 to 15 min, the mobile phase in which the concentration of the A phase is 60% is adopted from the 15 min to 35 min and the mobile phase in which the concentration of the B phase is 100% is adopted from the 35 min to 60 min, eluate from the 25 min to the 29 min is collected, subjected to vacuum concentration, dissolved and separated through the Silica normal-phase silica gel chromatographic column adopting the ternary mobile phase comprising the n-hexane as the A phase and the ethyl acetate as the B phase, elution is performed for 0-45 min with the concentration of the A phase being 88%, and eluate from the 27.5 min to the 36 min is collected and subjected to rotary evaporation to obtain the ergosterol the purity of which is 95% or above. The method is stable in separation, high in repeatability and high in preparation amount. The prepared ergosterol can be used for preparing anti-liver-cancer medicines.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a method for extracting ergosterol from Armillaria chrysanthemum and the application of the obtained ergosterol in the preparation of anti-liver cancer drugs. Background technique [0002] Ergosterol, also known as ergosterol, is an important phytosterol mainly present in yeast and ergot. Ergosterol is the precursor for the production of vitamin D2, has the function of vitamin D2, and is also an important part of microbial cell membranes. It plays an important role in ensuring the integrity of cell membranes, the activity of membrane-bound enzymes, the fluidity of membranes, cell viability, and the transportation of cell materials. plays an important role. [0003] Studies have shown that ergosterol can not only be used in the production of flavonoids and other drugs, but also that ergosterol is a safe natural drug with physiological activity and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00A61K31/575A61P35/00
Inventor 张耀洲马琳唐楚沉顾朋嫒李聪聪
Owner TIANJIN YAOYU BIOLOGICAL TECH
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