Quantitative detection method and device for determining pH value by using photoacoustic fluorescence signal ratio

Abstract

The invention discloses a quantitative detection method and device for measuring a pH value by utilizing an opto-acoustic fluorescence signal specific value. The quantitative detection device comprises an opto-acoustic fluorescence signal excitation source generator, an attenuation slice, an optical fiber coupler, a single mode fiber, a tunable collimator, a microobjective, a hollow cylindrical ultrasonic coupler, a photomultiplier, a filter, an amplifier, a dual channel parallel acquisition card, an ultrasonic coupling cup, a sample stage, a two-dimensional electric platform, an electromagnetic signal shielding metal case, a dichroscope, a reflector, an optical filter, a light resistant cylinder, and a computer with acquisition control software, image rebuilding software and motor control software. According to the quantitative detection method and device for measuring the pH value by utilizing the opto-acoustic fluorescence signal specific value provided by the invention, multiple parameters of a biological tissue can be obtained at the same time, and most importantly, the distribution of pH of the tissue can be quantified through statistical computing the obtained opto-acoustic fluorescence signals. The image resolution ratio can achieve 0.1 to 2 mum. Meanwhile, high fluorescent sensitivity, good photoacoustic resolution and good congruity of photoacoustic images and fluorescence images are integrated.

Description

technical field [0001] The invention belongs to a non-destructive test and measurement technology, in particular to a quantitative detection method and device for measuring pH value by using photoacoustic fluorescence signal ratio. Background technique [0002] The pH value is a special manifestation of vital signs. Since the current technology is still unable to achieve simultaneous photoacoustic fluorescence measurement of pH technology, the photoacoustic signal of deeper tissues can be measured through light excitation. Due to the low sensitivity of the photoacoustic signal, Therefore, we also detected the fluorescent signal emitted by the tissue at the same time, which made up for the shortcoming of the low sensitivity of the photoacoustic signal. Therefore, we invented this device and method for measuring the pH value of biological tissue by photoacoustic fluorescence [0003] Combining these two methods with imaging can obtain many parameters of the tissue, but a singl...

AI Technical Summary

Problems solved by technology

[0002] The pH value is a special manifestation of vital signs. Since the current technology is still unable to achieve simultaneous photoacoustic fluorescence measurement of pH technology, the photoacoustic signal of deeper tissues can be measured through light excitation. Due to the low sensitivity of the photoacoustic signal, Therefore, we also detected the fluorescent signal emitted by the tissue at the same time, which made up for the shortcoming of the low sensitivity of the photoacoustic signal. Therefore, we invented this device and method for measuring the pH value of biological tissue by photoacoustic fluorescence

[0003] Combining these two methods with imaging can obtain many parameters of the tissue, but a single photoacoustic or fluorescence cannot obtain the pH distribution of the tissue, and the ratio of the two has a great correlation with the pH value of the sample, so we use Qualified properties to quantify tissue pH

Images